CAST2_MOUSE
ID CAST2_MOUSE Reviewed; 329 AA.
AC Q8CAB8; Q8C7A9; Q9ER44;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Cytosolic arginine sensor for mTORC1 subunit 2 {ECO:0000250|UniProtKB:A6NHX0};
DE AltName: Full=GATS-like protein 2 {ECO:0000305};
GN Name=Castor2 {ECO:0000250|UniProtKB:A6NHX0};
GN Synonyms=Gats, Gatsl2 {ECO:0000312|MGI:MGI:1933384};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Perez-Jurado L., Pezzi N., Buesa J., Barbero J.;
RT "Cloning of cDNA encoding murine GATS protein.";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart, and Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Functions as a negative regulator of the TORC1 signaling
CC pathway through the GATOR complex. As part of homodimers or
CC heterodimers with CASTOR1, directly binds and inhibits the GATOR
CC subcomplex GATOR2 and thereby mTORC1. Does not directly bind arginine,
CC but binding of arginine to CASTOR1 disrupts the interaction of CASTOR2-
CC containing heterodimers with GATOR2 which can in turn activate mTORC1
CC and the TORC1 signaling pathway. {ECO:0000250|UniProtKB:A6NHX0}.
CC -!- SUBUNIT: Forms homodimers and heterodimers with CASTOR1. Interacts with
CC the GATOR2 complex which is composed of MIOS, SEC13, SEH1L, WDR24 and
CC WDR59; the interaction is not regulated by arginine.
CC {ECO:0000250|UniProtKB:A6NHX0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:A6NHX0}.
CC -!- SIMILARITY: Belongs to the GATS family. {ECO:0000305}.
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DR EMBL; AJ296173; CAC14178.1; -; mRNA.
DR EMBL; AK039122; BAC30244.1; -; mRNA.
DR EMBL; AK052217; BAC34889.1; -; mRNA.
DR EMBL; BC094613; AAH94613.1; -; mRNA.
DR CCDS; CCDS19717.1; -.
DR RefSeq; NP_109644.2; NM_030719.3.
DR AlphaFoldDB; Q8CAB8; -.
DR SMR; Q8CAB8; -.
DR STRING; 10090.ENSMUSP00000016088; -.
DR PhosphoSitePlus; Q8CAB8; -.
DR SwissPalm; Q8CAB8; -.
DR EPD; Q8CAB8; -.
DR MaxQB; Q8CAB8; -.
DR PaxDb; Q8CAB8; -.
DR PeptideAtlas; Q8CAB8; -.
DR PRIDE; Q8CAB8; -.
DR ProteomicsDB; 283679; -.
DR Antibodypedia; 74738; 32 antibodies from 7 providers.
DR Ensembl; ENSMUST00000016088; ENSMUSP00000016088; ENSMUSG00000015944.
DR GeneID; 80909; -.
DR KEGG; mmu:80909; -.
DR UCSC; uc008zva.1; mouse.
DR CTD; 729438; -.
DR MGI; MGI:1933384; Castor2.
DR VEuPathDB; HostDB:ENSMUSG00000015944; -.
DR eggNOG; ENOG502QV83; Eukaryota.
DR GeneTree; ENSGT00390000006208; -.
DR HOGENOM; CLU_057799_0_0_1; -.
DR InParanoid; Q8CAB8; -.
DR OMA; STHIRFF; -.
DR OrthoDB; 1166424at2759; -.
DR PhylomeDB; Q8CAB8; -.
DR TreeFam; TF331648; -.
DR Reactome; R-MMU-9639288; Amino acids regulate mTORC1.
DR BioGRID-ORCS; 80909; 1 hit in 75 CRISPR screens.
DR ChiTaRS; Gatsl2; mouse.
DR PRO; PR:Q8CAB8; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8CAB8; protein.
DR Bgee; ENSMUSG00000015944; Expressed in superior colliculus and 235 other tissues.
DR Genevisible; Q8CAB8; MM.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0061700; C:GATOR2 complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:1903577; P:cellular response to L-arginine; IBA:GO_Central.
DR GO; GO:1904262; P:negative regulation of TORC1 signaling; ISS:UniProtKB.
DR GO; GO:1902531; P:regulation of intracellular signal transduction; IBA:GO_Central.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR040778; CASTOR1_N.
DR InterPro; IPR027795; CASTOR_ACT_dom.
DR InterPro; IPR026249; CASTOR_fam.
DR Pfam; PF13840; ACT_7; 2.
DR Pfam; PF18700; Castor1_N; 1.
DR PRINTS; PR02078; GATSLIKEFMLY.
DR SUPFAM; SSF55021; SSF55021; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..329
FT /note="Cytosolic arginine sensor for mTORC1 subunit 2"
FT /id="PRO_0000325893"
FT DOMAIN 72..140
FT /note="ACT 1"
FT DOMAIN 262..322
FT /note="ACT 2"
FT CONFLICT 15
FT /note="V -> VV (in Ref. 1; CAC14178)"
FT /evidence="ECO:0000305"
FT CONFLICT 56
FT /note="D -> H (in Ref. 2; BAC34889)"
FT /evidence="ECO:0000305"
FT CONFLICT 70
FT /note="S -> T (in Ref. 2; BAC34889)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="W -> C (in Ref. 2; BAC34889)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="F -> L (in Ref. 2; BAC34889)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 329 AA; 36112 MW; E014364B1418E6AF CRC64;
MELHILEHRL QVASVAKESI PLFTYGLIKL AFLSSKTRCK FFSLTETPED YTIIVDEEGF
LELPSSEHLS VADATWLALN VVSGGGSFSS SQPIGVTKIA KSVIAPLADQ NISVFMLSTY
QTDFILVRER DLPFVTHTLS SEFTILRVVN GETVAAENLS FTNGFVKPKM VQRPVIHPLS
SPSNRFCVTS LDPDTLPAVA TLLMDVMFYS NGVKDPMAAS DDCGHIRFFS FSLIEGYISL
VMDVQTQQRF PSHLLFTSAS GELWKMVRIG GQPLGFDECG IVAQISEPLA AADIPAYYIS
TFKFDHALVP EENISGVIHA LKVSQAGKH
