ID   Y855_MYCLE              Reviewed;         445 AA.
AC   O32965; Q9CCF7;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Uncharacterized zinc protease ML0855;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=ML0855; ORFNames=MLCB22.26c;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC31236.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z98741; CAB11391.1; -; Genomic_DNA.
DR   EMBL; AL583920; CAC31236.1; ALT_INIT; Genomic_DNA.
DR   PIR; A87016; A87016.
DR   PIR; T44899; T44899.
DR   RefSeq; WP_010907968.1; NC_002677.1.
DR   AlphaFoldDB; O32965; -.
DR   SMR; O32965; -.
DR   STRING; 272631.ML0855; -.
DR   EnsemblBacteria; CAC31236; CAC31236; CAC31236.
DR   KEGG; mle:ML0855; -.
DR   Leproma; ML0855; -.
DR   eggNOG; COG0612; Bacteria.
DR   HOGENOM; CLU_009902_3_3_11; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   SUPFAM; SSF63411; SSF63411; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Zinc.
FT   CHAIN           1..445
FT                   /note="Uncharacterized zinc protease ML0855"
FT                   /id="PRO_0000074421"
FT   REGION          232..251
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        69
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         66
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         70
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT   BINDING         146
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
SQ   SEQUENCE   445 AA;  48156 MW;  9ADA04E5F31D33D8 CRC64;
     MRRSKQGAEG KAEKKAARSA GVCRTTLPGG LRVVTEHLPA VRSASVGVWV GVGSRDEGAT
     VAGAAHFLEH LLFKSTSTRT AMDIAQAIDA VGGELNAFTA KEHTCYYAHV LDSDLELAVD
     LVADVVLNGR CAVDDVELER DVVLEEIAMR DDDPEDALGD MFLAALFGDH PVGRPVIGTM
     ESVSAMTRTQ LHSFHVRRYT PERMVVAVAG NVDHDEMVAL VREHFGSRLI RGRQSAPPRK
     STGRINGGPA LTLGKRDAEQ THVLLGVRTP GRSWEHRWAL SVLHTALGGG LSSRLFQEIR
     ETRGLAYSVY SALDIFADSG ALSVYAACLP GRFADVMQVI SEVLASVAGD GITEAECRIA
     KGSLRGGIIL GLEDSNSWMS RLGRSELNYG KYRGIEHTLQ QIDEVTVEQV NALAHQLLNK
     RYGAAVLGPY ASKKTLPRQL RIMVN