CAS_ARATH
ID CAS_ARATH Reviewed; 387 AA.
AC Q9FN48;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Calcium sensing receptor, chloroplastic;
DE AltName: Full=Sulfurtransferase 3;
DE Short=AtStr3;
DE Flags: Precursor;
GN Name=CAS; Synonyms=STR3; OrderedLocusNames=At5g23060; ORFNames=MYJ24.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12968184; DOI=10.1038/nature01932;
RA Han S., Tang R., Anderson L.K., Woerner T.E., Pei Z.M.;
RT "A cell surface receptor mediates extracellular Ca(2+) sensing in guard
RT cells.";
RL Nature 425:196-200(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [6]
RP SUBCELLULAR LOCATION, PHOSPHORYLATION AT THR-380, AND DISRUPTION PHENOTYPE.
RX PubMed=18331354; DOI=10.1111/j.1742-4658.2008.06335.x;
RA Vainonen J.P., Sakuragi Y., Stael S., Tikkanen M., Allahverdiyeva Y.,
RA Paakkarinen V., Aro E., Suorsa M., Scheller H.V., Vener A.V., Aro E.M.;
RT "Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane
RT of Arabidopsis thaliana.";
RL FEBS J. 275:1767-1777(2008).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18507772; DOI=10.1111/j.1469-8137.2008.02492.x;
RA Weinl S., Held K., Schluecking K., Steinhorst L., Kuhlgert S., Hippler M.,
RA Kudla J.;
RT "A plastid protein crucial for Ca2+-regulated stomatal responses.";
RL New Phytol. 179:675-686(2008).
RN [8]
RP GENE FAMILY.
RX PubMed=17408957; DOI=10.1016/j.plaphy.2007.02.005;
RA Bartels A., Mock H.P., Papenbrock J.;
RT "Differential expression of Arabidopsis sulfurtransferases under various
RT growth conditions.";
RL Plant Physiol. Biochem. 45:178-187(2007).
RN [9]
RP FUNCTION.
RX PubMed=17347443; DOI=10.1126/science.1134457;
RA Tang R.H., Han S., Zheng H., Cook C.W., Choi C.S., Woerner T.E.,
RA Jackson R.B., Pei Z.M.;
RT "Coupling diurnal cytosolic Ca2+ oscillations to the CAS-IP3 pathway in
RT Arabidopsis.";
RL Science 315:1423-1426(2007).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [12]
RP FUNCTION.
RX PubMed=21940718; DOI=10.1093/jxb/err259;
RA Wang W.H., Yi X.Q., Han A.D., Liu T.W., Chen J., Wu F.H., Dong X.J.,
RA He J.X., Pei Z.M., Zheng H.L.;
RT "Calcium-sensing receptor regulates stomatal closure through hydrogen
RT peroxide and nitric oxide in response to extracellular calcium in
RT Arabidopsis.";
RL J. Exp. Bot. 63:177-190(2012).
RN [13]
RP FUNCTION, AND INDUCTION BY CALCIUM.
RX PubMed=21919914; DOI=10.1111/j.1399-3054.2011.01523.x;
RA Huang S.S., Chen J., Dong X.J., Patton J., Pei Z.M., Zheng H.L.;
RT "Calcium and calcium receptor CAS promote Arabidopsis thaliana de-
RT etiolation.";
RL Physiol. Plantarum 144:73-82(2012).
CC -!- FUNCTION: Modulates cytoplasmic Ca(2+) concentration and is crucial for
CC proper stomatal regulation in response to elevated levels of external
CC Ca(2+). May function by regulating concentrations of inositol 1,4,5-
CC trisphosphate (IP3), which in turn triggers release of Ca(2+) from
CC internal stores. May play a role in de-etiolation.
CC {ECO:0000269|PubMed:12968184, ECO:0000269|PubMed:17347443,
CC ECO:0000269|PubMed:18507772, ECO:0000269|PubMed:21919914,
CC ECO:0000269|PubMed:21940718}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:18331354, ECO:0000269|PubMed:18507772}; Single-pass
CC membrane protein {ECO:0000269|PubMed:18331354,
CC ECO:0000269|PubMed:18507772}; Stromal side
CC {ECO:0000269|PubMed:18331354, ECO:0000269|PubMed:18507772}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the shoot, including
CC guard cells. {ECO:0000269|PubMed:12968184}.
CC -!- INDUCTION: By Ca(2+) (at protein level). {ECO:0000269|PubMed:21919914}.
CC -!- PTM: Phosphorylation seems to be light-dependent.
CC {ECO:0000269|PubMed:18331354}.
CC -!- DISRUPTION PHENOTYPE: Reduced growth. {ECO:0000269|PubMed:18331354}.
CC -!- MISCELLANEOUS: Plants silencing CAS show reduced chlorophyll content
CC and early bolting. {ECO:0000305|PubMed:21919914}.
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DR EMBL; AY341888; AAQ73179.1; -; mRNA.
DR EMBL; AB006708; BAB09823.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93114.1; -; Genomic_DNA.
DR EMBL; AY045798; AAK76472.1; -; mRNA.
DR EMBL; AY079331; AAL85062.1; -; mRNA.
DR EMBL; BT000666; AAN31813.1; -; mRNA.
DR RefSeq; NP_197697.1; NM_122212.5.
DR AlphaFoldDB; Q9FN48; -.
DR SMR; Q9FN48; -.
DR BioGRID; 17645; 8.
DR IntAct; Q9FN48; 7.
DR MINT; Q9FN48; -.
DR STRING; 3702.AT5G23060.1; -.
DR iPTMnet; Q9FN48; -.
DR PaxDb; Q9FN48; -.
DR PRIDE; Q9FN48; -.
DR ProteomicsDB; 240284; -.
DR EnsemblPlants; AT5G23060.1; AT5G23060.1; AT5G23060.
DR GeneID; 832370; -.
DR Gramene; AT5G23060.1; AT5G23060.1; AT5G23060.
DR KEGG; ath:AT5G23060; -.
DR Araport; AT5G23060; -.
DR TAIR; locus:2178287; AT5G23060.
DR eggNOG; ENOG502QSV6; Eukaryota.
DR HOGENOM; CLU_040238_1_1_1; -.
DR OMA; PIWSAIS; -.
DR OrthoDB; 1224370at2759; -.
DR PhylomeDB; Q9FN48; -.
DR PRO; PR:Q9FN48; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FN48; baseline and differential.
DR Genevisible; Q9FN48; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; IDA:TAIR.
DR GO; GO:0071277; P:cellular response to calcium ion; IMP:UniProtKB.
DR GO; GO:0009704; P:de-etiolation; IMP:UniProtKB.
DR GO; GO:0090333; P:regulation of stomatal closure; IMP:TAIR.
DR Gene3D; 3.40.250.10; -; 1.
DR InterPro; IPR044690; CAS_plant.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR036873; Rhodanese-like_dom_sf.
DR PANTHER; PTHR34209; PTHR34209; 1.
DR SUPFAM; SSF52821; SSF52821; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Phosphoprotein; Plastid; Receptor;
KW Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..33
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 34..387
FT /note="Calcium sensing receptor, chloroplastic"
FT /id="PRO_0000401137"
FT TOPO_DOM 34..186
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..387
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT DOMAIN 231..352
FT /note="Rhodanese"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT MOD_RES 380
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18331354"
SQ SEQUENCE 387 AA; 41285 MW; 81A99499D582A107 CRC64;
MAMAEMATKS SLSAKLTLPS SSTKKTLSLR QVSVSLPTST SISLLSLFAS PPHEAKAAVS
IPKDQIVSSL TEVEKTINQV QETGSSVFDA TQRVFQVVGD ALKPALDTAL PIAKQAGEEA
MKLASPAFSE ASKKAQEAMQ SSGFDSEPVF NAAKTVTDVA QQTSKAIEDA KPIASSTMDT
ISSADPSVIV VAAGAAFLAY LLLPPVFSAI SFNFRGYKGD LTPAQTLDLL CTKNYLMVDI
RSEKDKEKAG IPRLPSNAKN RVISIPLEEL PNKVKGIVRN SKRVEAEIAA LKISYLKKIN
KGSNIIILDS YTDSAKIVAK TLKVLGYKNC YIVTDGFSGG RGWLQSRLGT DSYNFSFAQV
LSPSRIIPAA SRSFGTRSGT KFLPSSD
