CAS_DROME
ID CAS_DROME Reviewed; 793 AA.
AC Q7M3M8; Q9VNH0;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Transcription factor castor;
DE AltName: Full=Protein ming;
GN Name=cas; Synonyms=ming; ORFNames=CG2102;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=1418995; DOI=10.1016/0896-6273(92)90234-5;
RA Mellerick D.M., Kassis J.A., Zhang S.-D., Odenwald W.F.;
RT "Castor encodes a novel zinc finger protein required for the development of
RT a subset of CNS neurons in Drosophila.";
RL Neuron 9:789-803(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 281-660, FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=1339340; DOI=10.1242/dev.116.4.943;
RA Cui X., Doe C.Q.;
RT "ming is expressed in neuroblast sublineages and regulates gene expression
RT in the Drosophila central nervous system.";
RL Development 116:943-952(1992).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=7588058; DOI=10.1242/dev.121.10.3233;
RA Cui X., Doe C.Q.;
RT "The role of the cell cycle and cytokinesis in regulating neuroblast
RT sublineage gene expression in the Drosophila CNS.";
RL Development 121:3233-3243(1995).
RN [7]
RP FUNCTION AS A REPRESSOR, DNA-BINDING, AND SUBCELLULAR LOCATION.
RX PubMed=9436984; DOI=10.1101/gad.12.2.246;
RA Kambadur R., Koizumi K., Stivers C., Nagle J., Poole S.J., Odenwald W.F.;
RT "Regulation of POU genes by castor and hunchback establishes layered
RT compartments in the Drosophila CNS.";
RL Genes Dev. 12:246-260(1998).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=11335107; DOI=10.1016/s0925-4773(01)00312-4;
RA Hitier R., Chaminade M., Preat T.;
RT "The Drosophila castor gene is involved in postembryonic brain
RT development.";
RL Mech. Dev. 103:3-11(2001).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-211 AND SER-215, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
CC -!- FUNCTION: Transcription factor that specifies expression of key genes
CC in developing central nervous system (CNS). Essential for many, if not
CC all, late developing neuroblastoma (NB) sublineages. Binds to the 5'-
CC [CG]C[CT][CT]AAAAA[AT]-3' DNA sequence, like hb, suggesting that cas
CC and hb act as a late regulators in early and late CNS NB sublineage,
CC respectively. Acts by repressing expression of nub/pdm-1 and pdm2/pdm-2
CC POU genes, and restrict their pattern of expression in appropriate
CC cells. Required for a full expression of vvl/drifter and acj6/I-POU; it
CC is however unknown whether it directly activates these genes. Controls
CC engrailed (en) expression in the ventral nerve chord.
CC {ECO:0000269|PubMed:1339340, ECO:0000269|PubMed:1418995,
CC ECO:0000269|PubMed:9436984}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9436984}.
CC -!- TISSUE SPECIFICITY: Expressed in a specific subset of neuroblasts in
CC the ventral nerve cord and the procephalic region in the embryo.
CC Expressed in many, if not all, late delaminating NBs, and in early NBs,
CC but only after they have undergone several rounds of ganglion mother
CC cell-producing divisions. {ECO:0000269|PubMed:11335107,
CC ECO:0000269|PubMed:1339340, ECO:0000269|PubMed:1418995,
CC ECO:0000269|PubMed:7588058}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos. Expressed from blastoderm
CC embryos. Not expressed in first and second instar larvae. Weakly
CC expressed in third instar larvae. May be weakly expressed in adults.
CC {ECO:0000269|PubMed:1418995}.
CC -!- SEQUENCE CAUTION:
CC Sequence=L04487; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L04487; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AE014297; AAF51966.1; -; Genomic_DNA.
DR EMBL; AY051865; AAK93289.1; -; mRNA.
DR EMBL; Z15091; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A49129; A49129.
DR PIR; JH0797; JH0797.
DR RefSeq; NP_524677.1; NM_079938.4.
DR AlphaFoldDB; Q7M3M8; -.
DR BioGRID; 68781; 11.
DR IntAct; Q7M3M8; 5.
DR STRING; 7227.FBpp0078374; -.
DR iPTMnet; Q7M3M8; -.
DR PaxDb; Q7M3M8; -.
DR EnsemblMetazoa; FBtr0078725; FBpp0078374; FBgn0004878.
DR GeneID; 44018; -.
DR KEGG; dme:Dmel_CG2102; -.
DR UCSC; CG2102-RA; d. melanogaster.
DR CTD; 44018; -.
DR FlyBase; FBgn0004878; cas.
DR VEuPathDB; VectorBase:FBgn0004878; -.
DR eggNOG; KOG4377; Eukaryota.
DR GeneTree; ENSGT00390000008187; -.
DR InParanoid; Q7M3M8; -.
DR OMA; MTQNPSM; -.
DR OrthoDB; 448560at2759; -.
DR PhylomeDB; Q7M3M8; -.
DR BioGRID-ORCS; 44018; 0 hits in 1 CRISPR screen.
DR ChiTaRS; caz; fly.
DR GenomeRNAi; 44018; -.
DR PRO; PR:Q7M3M8; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0004878; Expressed in embryonic central brain (Drosophila) and 44 other tissues.
DR ExpressionAtlas; Q7M3M8; baseline and differential.
DR Genevisible; Q7M3M8; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007417; P:central nervous system development; IMP:UniProtKB.
DR GO; GO:0007402; P:ganglion mother cell fate determination; TAS:FlyBase.
DR GO; GO:0048699; P:generation of neurons; IMP:FlyBase.
DR GO; GO:0021782; P:glial cell development; IMP:FlyBase.
DR GO; GO:0016319; P:mushroom body development; IMP:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0014019; P:neuroblast development; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009791; P:post-embryonic development; IMP:FlyBase.
DR GO; GO:0040034; P:regulation of development, heterochronic; NAS:FlyBase.
DR GO; GO:2000177; P:regulation of neural precursor cell proliferation; IMP:FlyBase.
DR GO; GO:0045664; P:regulation of neuron differentiation; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase.
DR InterPro; IPR040373; CASZ1.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR12451; PTHR12451; 2.
DR SMART; SM00355; ZnF_C2H2; 4.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..793
FT /note="Transcription factor castor"
FT /id="PRO_0000046911"
FT ZN_FING 377..402
FT /note="C2H2-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 439..463
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 498..522
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 556..580
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT DNA_BIND 650..662
FT /note="A.T hook"
FT REGION 44..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 272..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 599..682
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..70
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..290
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 602..644
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 211
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 215
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT CONFLICT 54
FT /note="Q -> E (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="R -> A (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="A -> G (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="G -> R (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
FT CONFLICT 634
FT /note="S -> T (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
FT CONFLICT 710
FT /note="A -> R (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
FT CONFLICT 724
FT /note="Q -> R (in Ref. 1; L04487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 793 AA; 87925 MW; E35DE18DD3C37671 CRC64;
MSNQMEFIMQ LYMMNLMKQQ QQMQLQLPQQ QQQQQLAGYT YTQNEDISSS TSVQQQQQQQ
QEQLQQPQPD LRKTRTHKRI SSQNTNCSSS RSCSPNSNLI AFQPQQYPGA TAATPSTPNS
HPSNLVNQML LQSLPPLTQL MLQQQQQQHL LTTSNLLLTP THTPSSLGKQ DPLQHPLLLG
QFAGSEQMAT NNFLQSSTVT STPIEREKAA TPAPSAGATA GNLSAAQVKF EQESADDDED
DDKPLSSLTS CSSSGHTNAS SEKLLLSGVH PLESTTDSLD SPSMYTPVKQ PADSSYGLIT
PVDSDLTPNT PLQPTQTISL LTPPSSEQSK SLVSLSAASG LDALLQNEEV LKNLRKVSSY
LECENSLCRQ ENLREHFHCH EEPCQGKILS KKDDIIRHLK WHKKRKESLK LGFARFSSSD
DCAPAYGEGC AYNWKQTHYH CVYEHCPKVY VSTSDVQMHA NFHRKDSEIV NEGFRRFRAH
ETCRIEDCPF FGKKISHYHC CREGCTHTFK NKADMDKHKT YHLKDHQLKM DGFKKILKTE
VCPFDACKFS TVCNHIHCVR EGCDYILHSS SQMISHKRKH DRQDGEQAYQ QFKIKQDVEE
SSLDAMPQQQ QQQQQQQPTS LSQSQSSSSV CGGSNTSTPL SSLSAEHFLA RKRGRPPKKI
QLPADAQQSE AKRLKVEDES SNPAMLLPQS QPAAAVHPLT SGLFPGLLPA AAAPGVDPTA
PNFQLTHLMA LFQLQNPLFY QNLYPGMTQN SSMLGNLAAL SAASAAAAAA AAANGAGVQQ
PKAEFSFKPE FKE
