ID   Y865_METJA              Reviewed;         449 AA.
AC   Q58275;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Putative methylthiotransferase MJ0865;
DE            EC=2.-.-.-;
GN   OrderedLocusNames=MJ0865;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the methylthiotransferase family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98870.1; -; Genomic_DNA.
DR   PIR; A64408; A64408.
DR   AlphaFoldDB; Q58275; -.
DR   SMR; Q58275; -.
DR   STRING; 243232.MJ_0865; -.
DR   EnsemblBacteria; AAB98870; AAB98870; MJ_0865.
DR   KEGG; mja:MJ_0865; -.
DR   eggNOG; arCOG01356; Archaea.
DR   HOGENOM; CLU_600805_0_0_2; -.
DR   InParanoid; Q58275; -.
DR   OMA; THRGCLG; -.
DR   PhylomeDB; Q58275; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.80.30.20; -; 1.
DR   InterPro; IPR023979; CHP04014_B12-bd/rSAM.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01217; B12-binding_methylthiotransfer; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR04014; B12_SAM_MJ_0865; 1.
DR   PROSITE; PS01278; MTTASE_RADICAL; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..449
FT                   /note="Putative methylthiotransferase MJ0865"
FT                   /id="PRO_0000141759"
FT   DOMAIN          163..390
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         177
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00780"
FT   BINDING         181
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00780"
FT   BINDING         184
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00780"
SQ   SEQUENCE   449 AA;  50957 MW;  6A97907DAF81DBB6 CRC64;
     MNKIKEGANV RITIYSPEVY TYGAMLIGGI LKHKGYNVHL VRKIDKTLFL KSDVIIFSLY
     STLHILDKNI REAIDFIKKV RKNKTKVYVA GCVSTYPEII LNELNVDGVI VGEGEITTPK
     IIEGDKEGLA YKEGDEIVIN YPKEKPDLNH PLPLIPKDIE QQSIRGANVY IETHRGCLGN
     CTFCQVPKFF GKTIRSRDVE DVVEEVKAFK RAGAKRIAIS GGTGSLYAFK KSINRDKFFE
     LLEKVSEVIG KNNLSVPDMR VDYVDEEILE AIKNYTIGWV FYGIESGSDK ILKDMKKGTN
     REKNLDAIKL AKDCGVKVAG SFIVAYPTET EMDYLLTKDF IVDAELDDVF VSIAEPIPTT
     ELCDLVLSMP KEENLLYKMH EGEYRKLGLS EAEARCFDLL IHAEMWKSMP KPLTPQLYNL
     YLNEARIQGK DIRAITDLLF KYRDLLLKK