ID   Y869_BACLD              Reviewed;         176 AA.
AC   Q65MB1; Q62XQ4; Q65MB0;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Putative metal-dependent hydrolase BLi00869/BLi00870/BL03027 {ECO:0000255|HAMAP-Rule:MF_01256};
DE            EC=3.-.-.- {ECO:0000255|HAMAP-Rule:MF_01256};
GN   OrderedLocusNames=BLi00869/BLi00870, BL03027;
OS   Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 /
OS   NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=279010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC   / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX   PubMed=15383718; DOI=10.1159/000079829;
RA   Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P.,
RA   Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.;
RT   "The complete genome sequence of Bacillus licheniformis DSM13, an organism
RT   with great industrial potential.";
RL   J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC   / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX   PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA   Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA   Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B.,
RA   Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A.,
RA   Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M.;
RT   "Complete genome sequence of the industrial bacterium Bacillus
RT   licheniformis and comparisons with closely related Bacillus species.";
RL   Genome Biol. 5:R77.1-R77.12(2004).
CC   -!- FUNCTION: Possible metal-dependent hydrolase. {ECO:0000255|HAMAP-
CC       Rule:MF_01256}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01256};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01256};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- SIMILARITY: Belongs to the metal hydrolase YfiT family.
CC       {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- CAUTION: Could be the product of a pseudogene. Resulting from
CC       truncation by frameshift mutation. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AE017333; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
CC       Sequence=CP000002; Type=Frameshift; Note=Produces two separate ORFs.; Evidence={ECO:0000305};
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DR   EMBL; AE017333; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP000002; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   Proteomes; UP000000606; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.450; -; 1.
DR   HAMAP; MF_01256; YfiT_hydrol; 1.
DR   InterPro; IPR024775; DinB-like.
DR   InterPro; IPR034660; DinB/YfiT-like.
DR   InterPro; IPR023774; Put_metal_dep_hydrolase_YfiT.
DR   Pfam; PF12867; DinB_2; 1.
DR   SUPFAM; SSF109854; SSF109854; 1.
PE   5: Uncertain;
KW   Cytoplasm; Hydrolase; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..176
FT                   /note="Putative metal-dependent hydrolase
FT                   BLi00869/BLi00870/BL03027"
FT                   /id="PRO_0000162372"
FT   BINDING         65
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
FT   BINDING         158
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
FT   BINDING         162
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
SQ   SEQUENCE   176 AA;  20281 MW;  DED2AC27ABC912DB CRC64;
     MDQLKYPIGT FSKPLEADER ELEKWIGDLK QAPHLLRSAA AELNEEQLDT PYRTGGWAVR
     QVVHHLADSH MNGYIRSKLA LTEETPLIRT FDEQNWAGLT DARTLDPDLS LALLDTLHLR
     WAALXESLTE ADFEKAYIYP GTGEEMKLYQ ALALYVWHSQ HHIAHIKAHR KRMGWN