ID   CAT2_CLOK5              Reviewed;         429 AA.
AC   P38942; A5N1N0;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=4-hydroxybutyrate coenzyme A transferase;
DE            EC=2.8.3.-;
GN   Name=cat2; OrderedLocusNames=CKL_3018;
OS   Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=431943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Soehling B., Gottschalk G.;
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680;
RX   PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA   Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA   Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA   Hagemeier C., Thauer R.K., Gottschalk G.;
RT   "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT   metabolic features.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 149-429.
RX   PubMed=8550525; DOI=10.1128/jb.178.3.871-880.1996;
RA   Soehling B., Gottschalk G.;
RT   "Molecular analysis of the anaerobic succinate degradation pathway in
RT   Clostridium kluyveri.";
RL   J. Bacteriol. 178:871-880(1996).
CC   -!- SIMILARITY: Belongs to the acetyl-CoA hydrolase/transferase family.
CC       {ECO:0000305}.
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DR   EMBL; L21902; AAA92344.1; -; Genomic_DNA.
DR   EMBL; CP000673; EDK35026.1; -; Genomic_DNA.
DR   RefSeq; WP_012103361.1; NC_009706.1.
DR   AlphaFoldDB; P38942; -.
DR   SMR; P38942; -.
DR   STRING; 431943.CKL_3018; -.
DR   PRIDE; P38942; -.
DR   EnsemblBacteria; EDK35026; EDK35026; CKL_3018.
DR   KEGG; ckl:CKL_3018; -.
DR   eggNOG; COG0427; Bacteria.
DR   HOGENOM; CLU_030703_1_0_9; -.
DR   OMA; FFMGANV; -.
DR   OrthoDB; 319106at2; -.
DR   BioCyc; MetaCyc:MON-13467; -.
DR   Proteomes; UP000002411; Chromosome.
DR   GO; GO:0008775; F:acetate CoA-transferase activity; IEA:InterPro.
DR   GO; GO:0003986; F:acetyl-CoA hydrolase activity; IEA:InterPro.
DR   GO; GO:0006083; P:acetate metabolic process; IEA:InterPro.
DR   GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR   Gene3D; 3.40.1080.20; -; 1.
DR   InterPro; IPR026888; AcetylCoA_hyd_C.
DR   InterPro; IPR038460; AcetylCoA_hyd_C_sf.
DR   InterPro; IPR003702; ActCoA_hydro.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   Pfam; PF13336; AcetylCoA_hyd_C; 1.
DR   Pfam; PF02550; AcetylCoA_hydro; 1.
DR   SUPFAM; SSF100950; SSF100950; 2.
PE   3: Inferred from homology;
KW   Reference proteome; Transferase.
FT   CHAIN           1..429
FT                   /note="4-hydroxybutyrate coenzyme A transferase"
FT                   /id="PRO_0000215526"
FT   ACT_SITE        238
FT                   /note="5-glutamyl coenzyme A thioester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:B3EY95"
FT   BINDING         215..219
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250|UniProtKB:B3EY95"
FT   BINDING         336
FT                   /ligand="CoA"
FT                   /ligand_id="ChEBI:CHEBI:57287"
FT                   /evidence="ECO:0000250|UniProtKB:B3EY95"
FT   CONFLICT        65
FT                   /note="E -> V (in Ref. 1; AAA92344)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        85
FT                   /note="C -> S (in Ref. 1; AAA92344)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        96
FT                   /note="D -> V (in Ref. 1; AAA92344)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   429 AA;  47133 MW;  BA0E581AC42372D2 CRC64;
     MEWEEIYKEK LVTAEKAVSK IENHSRVVFA HAVGEPVDLV NALVKNKDNY IGLEIVHMVA
     MGKGEYTKEG MQRHFRHNAL FVGGCTRDAV NSGRADYTPC FFYEVPSLFK EKRLPVDVAL
     IQVSEPDKYG YCSFGVSNDY TKPAAESAKL VIAEVNKNMP RTLGDSFIHV SDIDYIVEAS
     HPLLELQPPK LGDVEKAIGE NCASLIEDGA TLQLGIGAIP DAVLLFLKNK KNLGIHSEMI
     SDGVMELVKA GVINNKKKTL HPGKIVVTFL MGTKKLYDFV NNNPMVETYS VDYVNNPLVI
     MKNDNMVSIN SCVQVDLMGQ VCSESIGLKQ ISGVGGQVDF IRGANLSKGG KAIIAIPSTA
     GKGKVSRITP LLDTGAAVTT SRNEVDYVVT EYGVAHLKGK TLRNRARALI NIAHPKFRES
     LMNEFKKRF