CAT4_AGRFC
ID CAT4_AGRFC Reviewed; 209 AA.
AC P23364;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Chloramphenicol acetyltransferase;
DE EC=2.3.1.28;
GN Name=cat; OrderedLocusNames=Atu4738; ORFNames=AGR_L_286;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2013403; DOI=10.1016/0378-1119(91)90112-o;
RA Tennigkeit J., Matzura H.;
RT "Nucleotide sequence analysis of a chloramphenicol-resistance determinant
RT from Agrobacterium tumefaciens and identification of its gene product.";
RL Gene 98:113-116(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: This enzyme is an effector of chloramphenicol resistance in
CC bacteria.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate +
CC CoA; Xref=Rhea:RHEA:18421, ChEBI:CHEBI:16730, ChEBI:CHEBI:17698,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.28;
CC -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC {ECO:0000305}.
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DR EMBL; M58472; AAA22081.1; -; Genomic_DNA.
DR EMBL; AE007870; AAK88712.1; -; Genomic_DNA.
DR PIR; AF3139; AF3139.
DR PIR; F98148; F98148.
DR PIR; JN0132; JN0132.
DR RefSeq; NP_355927.1; NC_003063.2.
DR RefSeq; WP_010974114.1; NG_047600.1.
DR AlphaFoldDB; P23364; -.
DR SMR; P23364; -.
DR EnsemblBacteria; AAK88712; AAK88712; Atu4738.
DR KEGG; ag:AAA22081; -.
DR KEGG; atu:Atu4738; -.
DR PATRIC; fig|176299.10.peg.4545; -.
DR eggNOG; COG0110; Bacteria.
DR HOGENOM; CLU_051638_5_3_5; -.
DR OMA; VGRYSYY; -.
DR PhylomeDB; P23364; -.
DR BioCyc; AGRO:ATU4738-MON; -.
DR Proteomes; UP000000813; Chromosome linear.
DR GO; GO:0008811; F:chloramphenicol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR001451; Hexapep.
DR InterPro; IPR018357; Hexapep_transf_CS.
DR InterPro; IPR011004; Trimer_LpxA-like_sf.
DR Pfam; PF00132; Hexapep; 1.
DR SUPFAM; SSF51161; SSF51161; 1.
DR PROSITE; PS00101; HEXAPEP_TRANSFERASES; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Antibiotic resistance; Reference proteome; Repeat;
KW Transferase.
FT CHAIN 1..209
FT /note="Chloramphenicol acetyltransferase"
FT /id="PRO_0000068657"
FT ACT_SITE 78
FT /evidence="ECO:0000250"
SQ SEQUENCE 209 AA; 23126 MW; 21BE2660CBE80DA2 CRC64;
MENYFESPFR GITLDKQVKS PNLVVGKYSY YSGYYHGHSF EDCARYLLPD EGADRLVIGS
FCSIGSGAAF IMAGNQGHRN EWISTFPFFF MPEVPEFENA ANGYLPAGDT VIGNDVWIGS
EAIIMPGITV GDGAVIGTRA LVTKDVEPYA IVGGNPAKTI RKRFDDDSIA LLLEMKWWGW
PAERLKAAMP LMTSGNVAAL YRFWRSDSL
