ID   CAT4_ECOLX              Reviewed;         210 AA.
AC   P26838;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Chloramphenicol acetyltransferase;
DE            EC=2.3.1.28;
GN   Name=catB2; Synonyms=cat;
OS   Escherichia coli.
OG   Plasmid IncFII NR79.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=Tn2424;
RX   PubMed=1314803; DOI=10.1128/jb.174.9.2891-2897.1992;
RA   Parent R., Roy P.H.;
RT   "The chloramphenicol acetyltransferase gene of Tn2424: a new breed of
RT   cat.";
RL   J. Bacteriol. 174:2891-2897(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=Tn2424;
RA   Gravel A., Parent R., Roy P.H.;
RL   Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   SEQUENCE REVISION TO 70.
RA   Gravel A., Parent R., Roy P.H.;
RL   Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This enzyme is an effector of chloramphenicol resistance in
CC       bacteria.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate +
CC         CoA; Xref=Rhea:RHEA:18421, ChEBI:CHEBI:16730, ChEBI:CHEBI:17698,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.28;
CC   -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC       {ECO:0000305}.
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DR   EMBL; AF047479; AAC14737.2; -; Genomic_DNA.
DR   PIR; A41857; A41857.
DR   RefSeq; WP_012477387.1; NZ_UGCJ01000005.1.
DR   AlphaFoldDB; P26838; -.
DR   SMR; P26838; -.
DR   KEGG; ag:AAC14737; -.
DR   GO; GO:0008811; F:chloramphenicol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR001451; Hexapep.
DR   InterPro; IPR018357; Hexapep_transf_CS.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   Pfam; PF00132; Hexapep; 1.
DR   SUPFAM; SSF51161; SSF51161; 1.
DR   PROSITE; PS00101; HEXAPEP_TRANSFERASES; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Plasmid; Repeat; Transferase;
KW   Transposable element.
FT   CHAIN           1..210
FT                   /note="Chloramphenicol acetyltransferase"
FT                   /id="PRO_0000068658"
FT   ACT_SITE        79
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   210 AA;  23531 MW;  4711B3E33450DABE CRC64;
     MTNYFESPFK GKLLTEQVKN PNIKVGRYSY YSGYYHGHSF DDCARYLLPD RDDVDQLIIG
     SFCSIGSGAA FIMAGNQGHR YDWVSSFPFF YMNEEPAFAK SVDAFQRAGD TVIGSDVWIG
     SEAMIMPGIK IGHGAVIGSR ALVAKDVEPY TIVGGNPAKS IRKRFSEEEI SMLLDMAWWD
     WPLEQIKEAM PFLCSSGIAS LYRRWQGTSA