ID   CAT4_KLEAE              Reviewed;         210 AA.
AC   P50868;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Chloramphenicol acetyltransferase;
DE            EC=2.3.1.28;
GN   Name=catB4;
OS   Klebsiella aerogenes (Enterobacter aerogenes).
OG   Plasmid pBWH301.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7793874; DOI=10.1128/aac.39.3.686;
RA   Bunny K.L., Hall R.M., Stokes H.W.;
RT   "New mobile gene cassettes containing an aminoglycoside resistance gene,
RT   aacA7, and a chloramphenicol resistance gene, catB3, in an integron in
RT   pBWH301.";
RL   Antimicrob. Agents Chemother. 39:686-693(1995).
CC   -!- FUNCTION: This enzyme is an effector of chloramphenicol resistance in
CC       bacteria.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + chloramphenicol = chloramphenicol 3-acetate +
CC         CoA; Xref=Rhea:RHEA:18421, ChEBI:CHEBI:16730, ChEBI:CHEBI:17698,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.28;
CC   -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC       {ECO:0000305}.
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DR   EMBL; U13880; AAA90938.1; -; Genomic_DNA.
DR   RefSeq; WP_000186237.1; NZ_MG288682.1.
DR   AlphaFoldDB; P50868; -.
DR   SMR; P50868; -.
DR   GeneID; 29368452; -.
DR   GO; GO:0008811; F:chloramphenicol O-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR001451; Hexapep.
DR   InterPro; IPR018357; Hexapep_transf_CS.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   Pfam; PF00132; Hexapep; 1.
DR   SUPFAM; SSF51161; SSF51161; 1.
DR   PROSITE; PS00101; HEXAPEP_TRANSFERASES; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Plasmid; Repeat; Transferase.
FT   CHAIN           1..210
FT                   /note="Chloramphenicol acetyltransferase"
FT                   /id="PRO_0000068659"
FT   ACT_SITE        79
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   210 AA;  23617 MW;  25422E5C0A15BF64 CRC64;
     MTNYFDSPFK GKLLSEQVKN PNIKVGRYSY YSGYYHGHSF DDCARYLFPD RDDVDKLIIG
     SFCSIGSGAS FIMAGNQGHR YDWASSFPFF YMQEEPAFSS ALDAFQKAGN TVIGNDVWIG
     SEAMVMPGIK IGHGAVIGSR SLVTKDVEPY AIVGGNPAKK IKKRFTDEEI SLLLEMEWWN
     WSLEKIKAAM PMLCSSNIVG LHKYWLEFAV