CAT8_YEAST
ID CAT8_YEAST Reviewed; 1433 AA.
AC P39113; D6W0A7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Regulatory protein CAT8;
GN Name=CAT8; Synonyms=MSP8; OrderedLocusNames=YMR280C; ORFNames=YM8021.06C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7891685; DOI=10.1128/mcb.15.4.1915;
RA Hedges D., Proft M., Entian K.-D.;
RT "CAT8, a new zinc cluster-encoding gene necessary for derepression of
RT gluconeogenic enzymes in the yeast Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 15:1915-1922(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ENY.WA-1A;
RA Boles E., Hettmann C., Zimmermann F.K.;
RL Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Activator of the gluconeogenic enzymes FBP1 and PCK1 genes.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- PTM: Could be the target of the SNF1/CAT1 - SNF4/CAT3 kinase complex.
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DR EMBL; X78344; CAA55139.1; -; Genomic_DNA.
DR EMBL; Z49704; CAA89778.1; -; Genomic_DNA.
DR EMBL; X94215; CAA63906.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10181.1; -; Genomic_DNA.
DR PIR; S54587; S54587.
DR RefSeq; NP_014007.1; NM_001182787.1.
DR AlphaFoldDB; P39113; -.
DR SMR; P39113; -.
DR BioGRID; 35459; 106.
DR DIP; DIP-4397N; -.
DR MINT; P39113; -.
DR STRING; 4932.YMR280C; -.
DR iPTMnet; P39113; -.
DR MaxQB; P39113; -.
DR PaxDb; P39113; -.
DR PRIDE; P39113; -.
DR EnsemblFungi; YMR280C_mRNA; YMR280C; YMR280C.
DR GeneID; 855323; -.
DR KEGG; sce:YMR280C; -.
DR SGD; S000004893; CAT8.
DR VEuPathDB; FungiDB:YMR280C; -.
DR eggNOG; ENOG502QTKQ; Eukaryota.
DR HOGENOM; CLU_004300_1_0_1; -.
DR InParanoid; P39113; -.
DR OMA; QCSQCAA; -.
DR BioCyc; YEAST:G3O-32951-MON; -.
DR PRO; PR:P39113; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P39113; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0061414; P:positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source; IMP:SGD.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Activator; Carbohydrate metabolism; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..1433
FT /note="Regulatory protein CAT8"
FT /id="PRO_0000114940"
FT DNA_BIND 70..97
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 20..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 936..1024
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1137..1162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1200..1236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1324..1433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 936..950
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 951..965
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 966..991
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 992..1009
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1324..1394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1407..1433
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 303
FT /note="S -> A (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 747
FT /note="K -> L (in Ref. 1; CAA55139)"
FT /evidence="ECO:0000305"
FT CONFLICT 768
FT /note="T -> A (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 999..1002
FT /note="Missing (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1008
FT /note="G -> S (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1016
FT /note="H -> Q (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1019
FT /note="Q -> P (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1061
FT /note="V -> M (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1072..1074
FT /note="TDA -> ADS (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1092
FT /note="N -> S (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1100
FT /note="I -> V (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1120
FT /note="M -> L (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
FT CONFLICT 1162
FT /note="S -> A (in Ref. 4; CAA63906)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1433 AA; 160485 MW; 5ED790BEFB47B632 CRC64;
MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM IKTASPTPLS
TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS DKLLRKAYPK GYTESLEERV
RELEAENKRL LALCDIKEQQ ISLVSQSRPQ TSTDNTINGN FKHDLKDAPL NLSSTNIYLL
NQTVNKQLQN GKMDGDNSGS AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT
AISFEQDEAP GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG
FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV KRFQSTNLSE
FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK ELLHLFFKFW SNQVPILNND
HFLIYFNNFV EVVKHLSTEN LETNNTTKST VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK
YTVPKNPKAK YARLMAYYHQ LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA
IYGVRGRIVS MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG
VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL QIIRFAKILG
NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY FQITVNGTVN LDEIRATNQR
NTETKFDKKD IILFEKKILL LFYFLAKSMI HLPVIATKPL PKNVDNATKK KQSMFNNDSK
GATNQDHMIL DVDMTSPAIR TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF
SLLCARGSLE YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL
KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR QRTPNVKREN
PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA KRNAKDGGIS KGENAHNFQN
DTKKNMSTSN LFPFSFSNTD LTALFTHPEG PNCTNTNNGN VDVCNRASTD ATDANIENLS
FLNMAPFLQT GNSNIGQNTI ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE
NSKNNQFHQK GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS
ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG NFNNFMTNVN
YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK NSIILDTTFN DDLDRSRMNA
REVLNPTDSI LSQGMVSSVS TRNTSNQRSL SSGNDSKGDS SSQENSKSAT GNQLDTPSTL
FQMRRTSSGP SASHRGPRRP QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK
