ID   Y895_MYCTO              Reviewed;         505 AA.
AC   P9WKA2; L0T7U3; P67204; Q10554;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase MT0919;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase MT0919;
GN   OrderedLocusNames=MT0919;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK45165.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK45165.1; ALT_INIT; Genomic_DNA.
DR   PIR; D70782; D70782.
DR   RefSeq; WP_003404663.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKA2; -.
DR   SMR; P9WKA2; -.
DR   EnsemblBacteria; AAK45165; AAK45165; MT0919.
DR   KEGG; mtc:MT0919; -.
DR   PATRIC; fig|83331.31.peg.987; -.
DR   HOGENOM; CLU_024186_4_1_11; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..505
FT                   /note="Putative diacyglycerol O-acyltransferase MT0919"
FT                   /id="PRO_0000427689"
FT   ACT_SITE        167
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   505 AA;  53873 MW;  626018C99918256C CRC64;
     MRQQQEADVV ALGRKPGLLC VPERFRAMDL PMAAADALFL WAETPTRPLH VGALAVLSQP
     DNGTGRYLRK VFSAAVARQQ VAPWWRRRPH RSLTSLGQWS WRTETEVDLD YHVRLSALPP
     RAGTAELWAL VSELHAGMLD RSRPLWQVDL IEGLPGGRCA VYVKVHHALA DGVSVMRLLQ
     RIVTADPHQR QMPTLWEVPA QASVAKHTAP RGSSRPLTLA KGVLGQARGV PGMVRVVADT
     TWRAAQCRSG PLTLAAPHTP LNEPIAGARS VAGCSFPIER LRQVAEHADA TINDVVLAMC
     GGALRAYLIS RGALPGAPLI AMVPVSLRDT AVIDVFGQGP GNKIGTLMCS LATHLASPVE
     RLSAIRASMR DGKAAIAGRS RNQALAMSAL GAAPLALAMA LGRVPAPLRP PNVTISNVPG
     PQGALYWNGA RLDALYLLSA PVDGAALNIT CSGTNEQITF GLTGCRRAVP ALSILTDQLA
     HELELLVGVS EAGPGTRLRR IAGRR