ID   Y895_MYCTU              Reviewed;         505 AA.
AC   P9WKA3; L0T7U3; P67204; Q10554;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase Rv0895;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase Rv0895;
GN   OrderedLocusNames=Rv0895; ORFNames=MTCY31.23;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   EXPRESSION IN E.COLI, AND INDUCTION BY NITRIC OXIDE (NO).
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15262939; DOI=10.1128/jb.186.15.5017-5030.2004;
RA   Daniel J., Deb C., Dubey V.S., Sirakova T.D., Abomoelak B., Morbidoni H.R.,
RA   Kolattukudy P.E.;
RT   "Induction of a novel class of diacylglycerol acyltransferases and
RT   triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into
RT   a dormancy-like state in culture.";
RL   J. Bacteriol. 186:5017-5030(2004).
CC   -!- FUNCTION: Upon expression in E.coli functions very weakly as a
CC       triacylglycerol synthase, making triacylglycerol (TG) from diolein and
CC       long-chain fatty acyl-CoA. Has no wax synthase activity.
CC       {ECO:0000269|PubMed:15262939}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- INDUCTION: Induced in response to low levels of nitric oxide (NO).
CC       {ECO:0000269|PubMed:15262939}.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP43643.1; -; Genomic_DNA.
DR   PIR; D70782; D70782.
DR   RefSeq; NP_215410.1; NC_000962.3.
DR   RefSeq; WP_003404663.1; NC_000962.3.
DR   AlphaFoldDB; P9WKA3; -.
DR   SMR; P9WKA3; -.
DR   STRING; 83332.Rv0895; -.
DR   PaxDb; P9WKA3; -.
DR   DNASU; 885481; -.
DR   GeneID; 885481; -.
DR   KEGG; mtu:Rv0895; -.
DR   TubercuList; Rv0895; -.
DR   eggNOG; COG1020; Bacteria.
DR   OMA; MSEDPHR; -.
DR   PhylomeDB; P9WKA3; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0001666; P:response to hypoxia; IBA:GO_Central.
DR   GO; GO:0071731; P:response to nitric oxide; IBA:GO_Central.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Reference proteome; Transferase.
FT   CHAIN           1..505
FT                   /note="Putative diacyglycerol O-acyltransferase Rv0895"
FT                   /id="PRO_0000222906"
FT   ACT_SITE        167
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   505 AA;  53873 MW;  626018C99918256C CRC64;
     MRQQQEADVV ALGRKPGLLC VPERFRAMDL PMAAADALFL WAETPTRPLH VGALAVLSQP
     DNGTGRYLRK VFSAAVARQQ VAPWWRRRPH RSLTSLGQWS WRTETEVDLD YHVRLSALPP
     RAGTAELWAL VSELHAGMLD RSRPLWQVDL IEGLPGGRCA VYVKVHHALA DGVSVMRLLQ
     RIVTADPHQR QMPTLWEVPA QASVAKHTAP RGSSRPLTLA KGVLGQARGV PGMVRVVADT
     TWRAAQCRSG PLTLAAPHTP LNEPIAGARS VAGCSFPIER LRQVAEHADA TINDVVLAMC
     GGALRAYLIS RGALPGAPLI AMVPVSLRDT AVIDVFGQGP GNKIGTLMCS LATHLASPVE
     RLSAIRASMR DGKAAIAGRS RNQALAMSAL GAAPLALAMA LGRVPAPLRP PNVTISNVPG
     PQGALYWNGA RLDALYLLSA PVDGAALNIT CSGTNEQITF GLTGCRRAVP ALSILTDQLA
     HELELLVGVS EAGPGTRLRR IAGRR