ID   Y916_MYCBO              Reviewed;         495 AA.
AC   P64746; A0A1R3XWS7; Q10532; X2BGG8;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Uncharacterized monooxygenase Mb0916;
DE            EC=1.14.13.-;
GN   OrderedLocusNames=BQ2027_MB0916;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the FAD-binding monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIT99514.1; -; Genomic_DNA.
DR   RefSeq; NP_854573.1; NC_002945.3.
DR   RefSeq; WP_003404651.1; NC_002945.4.
DR   AlphaFoldDB; P64746; -.
DR   SMR; P64746; -.
DR   EnsemblBacteria; SIT99514; SIT99514; BQ2027_MB0916.
DR   GeneID; 45424855; -.
DR   PATRIC; fig|233413.5.peg.997; -.
DR   OMA; CGCDVAS; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0004499; F:N,N-dimethylaniline monooxygenase activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR020946; Flavin_mOase-like.
DR   Pfam; PF00743; FMO-like; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN           1..495
FT                   /note="Uncharacterized monooxygenase Mb0916"
FT                   /id="PRO_0000186459"
FT   BINDING         16
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         36
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         45
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         56
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   BINDING         105
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   SITE            286
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   495 AA;  55039 MW;  08CB55F48674DB44 CRC64;
     MTGRCPTVAV VGAGMSGMCV AITLLSAGIT DVCIYEKADD VGGTWRDNTY PGLTCDVPSR
     LYQYSFAKNP NWTQMFSRGG EIQDYLRGIA ERYGLRHRIR FGATVVSARF DDGRWVLRTD
     SGTESTVDFL ISATGVLHHP RIPPIAGLDD FRGTVFHSAR WDHTVPLLGR RIAVIGTGST
     GVQLVCGLAG VAGKVTMFQR TAQWVLPWPN PRYSKLARVF HRAFPCLGSL AYKAYSLSFE
     TFAVALSNPG LHRKLVGAVC RASLRRVRDP RLRRALTPDY EPMCKRLVMS GGFYRAIQRD
     DVELVTAGID HVEHRGIVTD DGVLHEVDVI VLATGFDSHA FFRPMQLTGR DGIRIDDVWQ
     DGPHAHQTVA IPGFPNFFMM LGPHSPVGNF PLTAVAESQA EHIVQWIKRW RHGEFDTMEP
     KSAATEAYNT VLRAAMPNTV WTTGCDSWYL NKDGIPEVWP FAPAKHRAML ANLHPEEYDL
     RRYAAVRATS RPQSA