ID   Y919_MYCBO              Reviewed;         505 AA.
AC   P67205; A0A1R3XWR7; Q10554; X2BGC4;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Putative diacyglycerol O-acyltransferase Mb0919;
DE            EC=2.3.1.20 {ECO:0000250|UniProtKB:P9WKC9};
DE   AltName: Full=Putative triacylglycerol synthase Mb0919;
GN   OrderedLocusNames=BQ2027_MB0919;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC         + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC         Evidence={ECO:0000250|UniProtKB:P9WKC9};
CC   -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC   -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIT99517.1; -; Genomic_DNA.
DR   RefSeq; NP_854576.1; NC_002945.3.
DR   RefSeq; WP_003404663.1; NC_002945.4.
DR   AlphaFoldDB; P67205; -.
DR   SMR; P67205; -.
DR   PRIDE; P67205; -.
DR   EnsemblBacteria; SIT99517; SIT99517; BQ2027_MB0919.
DR   PATRIC; fig|233413.5.peg.1000; -.
DR   OMA; MSEDPHR; -.
DR   UniPathway; UPA00282; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR   InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR   InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR   PANTHER; PTHR31650; PTHR31650; 1.
DR   Pfam; PF03007; WES_acyltransf; 1.
DR   Pfam; PF06974; WS_DGAT_C; 1.
DR   TIGRFAMs; TIGR02946; acyl_WS_DGAT; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Glycerol metabolism; Lipid biosynthesis; Lipid metabolism;
KW   Transferase.
FT   CHAIN           1..505
FT                   /note="Putative diacyglycerol O-acyltransferase Mb0919"
FT                   /id="PRO_0000222907"
FT   ACT_SITE        167
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   505 AA;  53873 MW;  626018C99918256C CRC64;
     MRQQQEADVV ALGRKPGLLC VPERFRAMDL PMAAADALFL WAETPTRPLH VGALAVLSQP
     DNGTGRYLRK VFSAAVARQQ VAPWWRRRPH RSLTSLGQWS WRTETEVDLD YHVRLSALPP
     RAGTAELWAL VSELHAGMLD RSRPLWQVDL IEGLPGGRCA VYVKVHHALA DGVSVMRLLQ
     RIVTADPHQR QMPTLWEVPA QASVAKHTAP RGSSRPLTLA KGVLGQARGV PGMVRVVADT
     TWRAAQCRSG PLTLAAPHTP LNEPIAGARS VAGCSFPIER LRQVAEHADA TINDVVLAMC
     GGALRAYLIS RGALPGAPLI AMVPVSLRDT AVIDVFGQGP GNKIGTLMCS LATHLASPVE
     RLSAIRASMR DGKAAIAGRS RNQALAMSAL GAAPLALAMA LGRVPAPLRP PNVTISNVPG
     PQGALYWNGA RLDALYLLSA PVDGAALNIT CSGTNEQITF GLTGCRRAVP ALSILTDQLA
     HELELLVGVS EAGPGTRLRR IAGRR