ID   Y932_STRR6              Reviewed;         543 AA.
AC   Q8DPY7;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Uncharacterized RNA methyltransferase spr0932;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=spr0932;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AE007317; AAK99736.1; -; Genomic_DNA.
DR   PIR; D97988; D97988.
DR   RefSeq; NP_358526.1; NC_003098.1.
DR   RefSeq; WP_000914581.1; NC_003098.1.
DR   AlphaFoldDB; Q8DPY7; -.
DR   SMR; Q8DPY7; -.
DR   STRING; 171101.spr0932; -.
DR   DNASU; 933763; -.
DR   EnsemblBacteria; AAK99736; AAK99736; spr0932.
DR   GeneID; 60233396; -.
DR   KEGG; spr:spr0932; -.
DR   PATRIC; fig|171101.6.peg.1019; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; FYAGDMK; -.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0070041; F:rRNA (uridine-C5-)-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..543
FT                   /note="Uncharacterized RNA methyltransferase spr0932"
FT                   /id="PRO_0000162033"
FT   DOMAIN          1..59
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        408
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         283
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         312
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         333
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         381
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   543 AA;  61805 MW;  F164A3764B052BFF CRC64;
     MLKKNDIVEV EIVDLTHEGA GVAKVDGLVF FVENALPSEK ILMRVLKVNK KIGFGKVEKY
     LVQSPHRNQD LDLAYLRSGI ADLGHLSYPE QLKFKTKQVK DSLYKIAGIA DVEVAETLGM
     EHPVKYRNKA QVPVRRVNGV LETGFFRKNS HNLMPLEDFF IQDPVIDQVV VALRDLLRRF
     DLKPYDEKEQ SGLIRNLVVR RGHYSGQIMV VLVTTRPKVF RVDQLIEQVI KQFPEIVSVM
     QNINDQNTNA IFGKEWRTLY GQDYITDQML GNDFQIAGLA FYQVNTEMAE KLYQTAIDFA
     ELKKDDVVID AYSGIGTIGL SVAKHVKEVY GVELIPEAVE NSKKNAQLNN ISNAHYVCDT
     AENAMKNWLK DGIQPTVILV DPPRKGLTES FIKASAQTGA DRIAYISCNV ATMARDIKLY
     QELGYELKKV QPVDLFPQTH HVETVALLSK LDVDKHISVE IELDEMDLTS AESKATYAQI
     KEYVWNKFEL KVSTLYIAQI KKKCGIELRE HYNKSKKDKQ IIPQCTPEKE EAIMDALRHF
     KMI