Y947_ARCFU
ID Y947_ARCFU Reviewed; 132 AA.
AC O29315;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Putative RNase AF_0947;
DE EC=3.1.-.- {ECO:0000250|UniProtKB:Q8ECH6};
DE AltName: Full=Putative toxin AF_0947;
GN OrderedLocusNames=AF_0947;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
CC -!- FUNCTION: Probable toxic component of a putative type VII toxin-
CC antitoxin (TA) system, probably an RNase. Probably neutralized by
CC cognate antitoxin AF_0948. Neutralization may be due to AMPylation by
CC AF_0948. {ECO:0000250|UniProtKB:Q8ECH6}.
CC -!- SUBUNIT: Homodimer, probably forms a complex with cognate antitoxin
CC AF_0948. {ECO:0000250|UniProtKB:Q8ECH6}.
CC -!- PTM: Modified by cognate antitoxin AF_0948; probably at least 2
CC successive AMPylation events occur on Tyr-98.
CC {ECO:0000250|UniProtKB:A0A0B0QJR1}.
CC -!- SIMILARITY: Belongs to the HepT RNase toxin family. {ECO:0000305}.
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DR EMBL; AE000782; AAB90296.1; -; Genomic_DNA.
DR PIR; C69368; C69368.
DR RefSeq; WP_010878447.1; NC_000917.1.
DR AlphaFoldDB; O29315; -.
DR SMR; O29315; -.
DR STRING; 224325.AF_0947; -.
DR EnsemblBacteria; AAB90296; AAB90296; AF_0947.
DR GeneID; 1484170; -.
DR KEGG; afu:AF_0947; -.
DR eggNOG; arCOG02109; Archaea.
DR HOGENOM; CLU_152343_0_0_2; -.
DR OMA; MDIVAML; -.
DR OrthoDB; 113565at2157; -.
DR PhylomeDB; O29315; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR Gene3D; 1.20.120.580; -; 1.
DR InterPro; IPR008201; HepT-like.
DR InterPro; IPR037038; HepT-like_sf.
DR Pfam; PF01934; DUF86; 1.
PE 3: Inferred from homology;
KW Hydrolase; Nuclease; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..132
FT /note="Putative RNase AF_0947"
FT /id="PRO_0000158266"
FT MOTIF 91..98
FT /note="RX(4)HXY motif"
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT ACT_SITE 91
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT ACT_SITE 96
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
FT MOD_RES 98
FT /note="O-di-AMP-tyrosine"
FT /evidence="ECO:0000250|UniProtKB:A0A0B0QJR1"
SQ SEQUENCE 132 AA; 15408 MW; E599B94F19D7E1F7 CRC64;
MRRRRYLEKL EWVEREINFA TEHAMSDEVR KRAVLYSIMT AVEVVMDIVA MLVKDLGKQV
EDDYTNISKL LEECVIEESE AELLRRYNGL RNAIAHHYNH LDLSKVERAL TQLDELYEVA
VKLVKTADKL AG
