YAB1_ARATH
ID YAB1_ARATH Reviewed; 229 AA.
AC O22152;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Axial regulator YABBY 1;
DE AltName: Full=Fl-54;
DE AltName: Full=Protein ABNORMAL FLORAL ORGANS;
DE AltName: Full=Protein FILAMENTOUS FLOWER;
DE AltName: Full=Protein antherless;
GN Name=YAB1; Synonyms=AFO, AT, FIL; OrderedLocusNames=At2g45190;
GN ORFNames=F4L23.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF CYS-30; CYS-33; ASN-178 AND
RP 179-TRP--LYS-229.
RX PubMed=10457020; DOI=10.1242/dev.126.18.4117;
RA Siegfried K.R., Eshed Y., Baum S.F., Otsuga D., Drews G.N., Bowman J.L.;
RT "Members of the YABBY gene family specify abaxial cell fate in
RT Arabidopsis.";
RL Development 126:4117-4128(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, COFACTOR, AND MUTAGENESIS OF CYS-30.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10323860; DOI=10.1101/gad.13.9.1079;
RA Sawa S., Watanabe K., Goto K., Kanaya E., Morita E.H., Okada K.;
RT "FILAMENTOUS FLOWER, a meristem and organ identity gene of Arabidopsis,
RT encodes a protein with a zinc finger and HMG-related domains.";
RL Genes Dev. 13:1079-1088(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX AGRICOLA=IND22028778; DOI=10.1007/s004970050180;
RA Kumaran M.K., Ye D., Yang W.-C., Griffith M.E., Chaudhury A.M.,
RA Sundaresan V.;
RT "Molecular cloning of ABNORMAL FLORAL ORGANS: a gene required for flower
RT development in Arabidopsis.";
RL Sex. Plant Reprod. 12:118-122(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP FUNCTION.
RA Komaki M.K., Okada K., Nishino E., Shimura Y.;
RT "Isolation and characterization of novel mutants of Arabidopsis thaliana
RT defective in flower development.";
RL Development 104:195-203(1988).
RN [7]
RP FUNCTION.
RX PubMed=10331982; DOI=10.1242/dev.126.12.2715;
RA Chen Q., Atkinson A., Otsuga D., Christensen T., Reynolds L., Drews G.N.;
RT "The Arabidopsis FILAMENTOUS FLOWER gene is required for flower
RT formation.";
RL Development 126:2715-2726(1999).
RN [8]
RP FUNCTION.
RX PubMed=9878633; DOI=10.2307/3870839;
RA Sawa S., Ito T., Shimura Y., Okada K.;
RT "FILAMENTOUS FLOWER controls the formation and development of Arabidopsis
RT inflorescences and floral meristems.";
RL Plant Cell 11:69-86(1999).
RN [9]
RP SUBUNIT, COFACTOR, DOMAIN, AND MUTAGENESIS OF CYS-56.
RX PubMed=11092895; DOI=10.1074/jbc.m009491200;
RA Kanaya E., Watanabe K., Nakajima N., Okada K., Shimura Y.;
RT "Zinc release from the CH2C6 zinc finger domain of FILAMENTOUS FLOWER
RT protein from Arabidopsis thaliana induces self-assembly.";
RL J. Biol. Chem. 276:7383-7390(2001).
RN [10]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10679447; DOI=10.1016/s1369-5266(99)00035-7;
RA Bowman J.L.;
RT "The YABBY gene family and abaxial cell fate.";
RL Curr. Opin. Plant Biol. 3:17-22(2000).
RN [11]
RP FUNCTION, SUBUNIT, AND DOMAIN.
RX PubMed=11812777; DOI=10.1074/jbc.m108889200;
RA Kanaya E., Nakajima N., Okada K.;
RT "Non-sequence-specific DNA binding by the FILAMENTOUS FLOWER protein from
RT Arabidopsis thaliana is reduced by EDTA.";
RL J. Biol. Chem. 277:11957-11964(2002).
RN [12]
RP FUNCTION.
RX PubMed=12417699; DOI=10.1105/tpc.004911;
RA Kumaran M.K., Bowman J.L., Sundaresan V.;
RT "YABBY polarity genes mediate the repression of KNOX homeobox genes in
RT Arabidopsis.";
RL Plant Cell 14:2761-2770(2002).
RN [13]
RP TISSUE SPECIFICITY.
RX PubMed=14555697; DOI=10.1105/tpc.015214;
RA Watanabe K., Okada K.;
RT "Two discrete cis elements control the abaxial side-specific expression of
RT the FILAMENTOUS FLOWER gene in Arabidopsis.";
RL Plant Cell 15:2592-2602(2003).
RN [14]
RP INTERACTION WITH SPL/NZZ.
RX PubMed=15299139; DOI=10.1104/pp.104.040154;
RA Sieber P., Petrascheck M., Barberis A., Schneitz K.;
RT "Organ polarity in Arabidopsis. NOZZLE physically interacts with members of
RT the YABBY family.";
RL Plant Physiol. 135:2172-2185(2004).
RN [15]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH LUG AND LUH.
RX PubMed=19837869; DOI=10.1105/tpc.109.070458;
RA Stahle M.I., Kuehlich J., Staron L., von Arnim A.G., Golz J.F.;
RT "YABBYs and the transcriptional corepressors LEUNIG and LEUNIG_HOMOLOG
RT maintain leaf polarity and meristem activity in Arabidopsis.";
RL Plant Cell 21:3105-3118(2009).
RN [16]
RP INTERACTION WITH SPL/NZZ AND SPEAR2.
RX PubMed=25527103; DOI=10.1016/j.jgg.2014.08.009;
RA Chen G.H., Sun J.Y., Liu M., Liu J., Yang W.C.;
RT "SPOROCYTELESS is a novel embryophyte-specific transcription repressor that
RT interacts with TPL and TCP proteins in Arabidopsis.";
RL J. Genet. Genomics 41:617-625(2014).
CC -!- FUNCTION: Involved in the abaxial cell fate determination during
CC embryogenesis and organogenesis. Regulates the initiation of embryonic
CC shoot apical meristem (SAM) development (PubMed:10323860,
CC PubMed:10331982, PubMed:10457020, PubMed:11812777, PubMed:12417699,
CC PubMed:9878633, Ref.3, Ref.6, PubMed:19837869). Required during flower
CC formation and development, particularly for the patterning of floral
CC organs. Positive regulator of class B (AP3 and PI) activity in whorls 2
CC and 3. Negative regulator of class B activity in whorl 1 and of SUP
CC activity in whorl 3. Interacts with class A proteins (AP1, AP2 and LUG)
CC to repress class C (AG) activity in whorls 1 and 2. Contributes to the
CC repression of KNOX genes (STM, KNAT1/BP and KNAT2) to avoid ectopic
CC meristems. Binds DNA without sequence specificity. In vitro, can
CC compete and displace the AP1 protein binding to DNA containing CArG box
CC (PubMed:10323860, PubMed:10331982, PubMed:10457020, PubMed:11812777,
CC PubMed:12417699, PubMed:9878633, Ref.3, Ref.6).
CC {ECO:0000269|PubMed:10323860, ECO:0000269|PubMed:10331982,
CC ECO:0000269|PubMed:10457020, ECO:0000269|PubMed:11812777,
CC ECO:0000269|PubMed:12417699, ECO:0000269|PubMed:19837869,
CC ECO:0000269|PubMed:9878633, ECO:0000269|Ref.3, ECO:0000269|Ref.6}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:10323860, ECO:0000269|PubMed:11092895};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000269|PubMed:10323860,
CC ECO:0000269|PubMed:11092895};
CC -!- SUBUNIT: Monomer and homomultimers (more than 20 subunits in vitro).
CC Interacts with DNA as monomer. The release of 1 zinc ion from a monomer
CC disrupts the binding to DNA and leads to multimerization. Interacts
CC with SPL/NZZ (PubMed:15299139, PubMed:25527103). Interacts with SPEAR2
CC (PubMed:25527103). Binds to LUG and LUH; these complexes promote
CC adaxial cell identity in leaves as well as embryonic shoot apical
CC meristem (SAM) initiation and postembryonic SAM maintenance
CC (PubMed:19837869). {ECO:0000269|PubMed:11092895,
CC ECO:0000269|PubMed:11812777, ECO:0000269|PubMed:15299139,
CC ECO:0000269|PubMed:19837869, ECO:0000269|PubMed:25527103}.
CC -!- INTERACTION:
CC O22152; Q9MAI5: ERF8; NbExp=3; IntAct=EBI-1113627, EBI-2000137;
CC O22152; Q9C9X7: IDD14; NbExp=4; IntAct=EBI-1113627, EBI-15191579;
CC O22152; O81836: SPL; NbExp=8; IntAct=EBI-1113627, EBI-1113588;
CC O22152; O82277: TCP10; NbExp=3; IntAct=EBI-1113627, EBI-3133327;
CC O22152; Q9LVI4: TIFY6B; NbExp=3; IntAct=EBI-1113627, EBI-1792431;
CC O22152; Q39262: ZFP3; NbExp=3; IntAct=EBI-1113627, EBI-15200178;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10323860}.
CC -!- TISSUE SPECIFICITY: Expressed in abaxial regions of young lateral
CC aerial organs, and in primordia leading to cotyledons, leaves, flower
CC meristems, sepals, petals, stamen and carpels, but not in roots.
CC {ECO:0000269|PubMed:10323860, ECO:0000269|PubMed:10457020,
CC ECO:0000269|PubMed:14555697, ECO:0000269|Ref.3}.
CC -!- DEVELOPMENTAL STAGE: Expressed in subepidermal cells of anlagen
CC regions, then in abaxial part of primordia and finally in
CC differentiating organs. Levels decrease in differentiated organs. In
CC embryo the expression starts during the transition between globular and
CC heart stages in the cotyledon anlagen. From the heart stage, expression
CC expands to abaxial domain of the cotyledon primordia and decrease as
CC the embryo matures. In stamen, expression restricted to the abaxial
CC region differentiating into the connective. In gynoecium, expressed in
CC the abaxial cell layers differentiating into the valves.
CC {ECO:0000269|PubMed:10457020}.
CC -!- DOMAIN: The non-canonical Cys-56 facilitates the zinc ion release from
CC the zinc finger domain. {ECO:0000269|PubMed:11092895,
CC ECO:0000269|PubMed:11812777}.
CC -!- DISRUPTION PHENOTYPE: Leaves polarity and growth defects.
CC {ECO:0000269|PubMed:19837869}.
CC -!- SIMILARITY: Belongs to the YABBY family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF136538; AAD33715.1; -; mRNA.
DR EMBL; AF074948; AAC69834.1; -; mRNA.
DR EMBL; AF087015; AAD16053.1; -; mRNA.
DR EMBL; AC002387; AAB82644.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10523.1; -; Genomic_DNA.
DR PIR; T51587; T51587.
DR RefSeq; NP_566037.1; NM_130082.4.
DR AlphaFoldDB; O22152; -.
DR SMR; O22152; -.
DR BioGRID; 4463; 26.
DR IntAct; O22152; 25.
DR STRING; 3702.AT2G45190.1; -.
DR PaxDb; O22152; -.
DR PRIDE; O22152; -.
DR EnsemblPlants; AT2G45190.1; AT2G45190.1; AT2G45190.
DR GeneID; 819127; -.
DR Gramene; AT2G45190.1; AT2G45190.1; AT2G45190.
DR KEGG; ath:AT2G45190; -.
DR Araport; AT2G45190; -.
DR TAIR; locus:2005492; AT2G45190.
DR eggNOG; ENOG502QQ88; Eukaryota.
DR HOGENOM; CLU_071156_0_1_1; -.
DR InParanoid; O22152; -.
DR OMA; VPCSSFF; -.
DR OrthoDB; 1256251at2759; -.
DR PhylomeDB; O22152; -.
DR PRO; PR:O22152; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22152; baseline and differential.
DR Genevisible; O22152; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010158; P:abaxial cell fate specification; IGI:TAIR.
DR GO; GO:0045165; P:cell fate commitment; IMP:TAIR.
DR GO; GO:0010154; P:fruit development; IMP:TAIR.
DR GO; GO:0010450; P:inflorescence meristem growth; IMP:TAIR.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; IMP:UniProtKB.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:2000024; P:regulation of leaf development; IMP:UniProtKB.
DR GO; GO:1902183; P:regulation of shoot apical meristem development; IMP:UniProtKB.
DR GO; GO:0010093; P:specification of floral organ identity; IMP:TAIR.
DR GO; GO:0090706; P:specification of plant organ position; IMP:TAIR.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR006780; YABBY.
DR PANTHER; PTHR31675; PTHR31675; 1.
DR Pfam; PF04690; YABBY; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..229
FT /note="Axial regulator YABBY 1"
FT /id="PRO_0000133717"
FT ZN_FING 30..57
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 45..107
FT /note="Involved in self-interaction"
FT REGION 120..180
FT /note="Participates in DNA-binding"
FT MUTAGEN 30
FT /note="C->Y: In fil-2; abnormal abaxial determination
FT during embryogenesis and organogenesis, filamentous
FT flowers."
FT /evidence="ECO:0000269|PubMed:10323860,
FT ECO:0000269|PubMed:10457020"
FT MUTAGEN 33
FT /note="C->Y: In fil-3; abnormal abaxial determination
FT during embryogenesis and organogenesis, filamentous
FT flowers."
FT /evidence="ECO:0000269|PubMed:10457020"
FT MUTAGEN 56
FT /note="C->A: Loss of zinc release and reduction of
FT multimerization."
FT /evidence="ECO:0000269|PubMed:11092895"
FT MUTAGEN 178
FT /note="N->K: In fil-4; abnormal abaxial determination
FT during embryogenesis and organogenesis, filamentous
FT flowers."
FT /evidence="ECO:0000269|PubMed:10457020"
FT MUTAGEN 179..229
FT /note="Missing: In fil-7; abnormal abaxial determination
FT during embryogenesis and organogenesis, filamentous
FT flowers."
FT /evidence="ECO:0000269|PubMed:10457020"
SQ SEQUENCE 229 AA; 25779 MW; 534FA1BC4734C5CF CRC64;
MSMSSMSSPS SAVCSPDHFS PSDHLCYVQC NFCQTILAVN VPYTSLFKTV TVRCGCCTNL
LSVNMRSYVL PASNQLQLQL GPHSYFNPQD ILEELRDAPS NMNMMMMNQH PTMNDIPSFM
DLHQQHEIPK APPVNRPPEK RQRVPSAYNR FIKEEIQRIK AGNPDISHRE AFSAAAKNWA
HFPHIHFGLV PDNQPVKKTN MPQQEGEDNM VMKEGFYAPA AANVGVTPY
