ID   YACG_ACTP7              Reviewed;          62 AA.
AC   B3H1L4;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=DNA gyrase inhibitor YacG {ECO:0000255|HAMAP-Rule:MF_00649};
GN   Name=yacG {ECO:0000255|HAMAP-Rule:MF_00649}; OrderedLocusNames=APP7_0934;
OS   Actinobacillus pleuropneumoniae serotype 7 (strain AP76).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Actinobacillus.
OX   NCBI_TaxID=537457;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AP76;
RA   Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N.,
RA   Tegetmeyer H., Singh M., Gerlach G.F.;
RT   "Genome and proteome analysis of A. pleuropneumoniae serotype 7.";
RL   Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Inhibits all the catalytic activities of DNA gyrase by
CC       preventing its interaction with DNA. Acts by binding directly to the C-
CC       terminal domain of GyrB, which probably disrupts DNA binding by the
CC       gyrase. {ECO:0000255|HAMAP-Rule:MF_00649}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00649};
CC       Note=Binds 1 zinc ion. {ECO:0000255|HAMAP-Rule:MF_00649};
CC   -!- SUBUNIT: Interacts with GyrB. {ECO:0000255|HAMAP-Rule:MF_00649}.
CC   -!- SIMILARITY: Belongs to the DNA gyrase inhibitor YacG family.
CC       {ECO:0000255|HAMAP-Rule:MF_00649}.
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DR   EMBL; CP001091; ACE61586.1; -; Genomic_DNA.
DR   RefSeq; WP_005597388.1; NC_010939.1.
DR   AlphaFoldDB; B3H1L4; -.
DR   SMR; B3H1L4; -.
DR   EnsemblBacteria; ACE61586; ACE61586; APP7_0934.
DR   GeneID; 66259755; -.
DR   KEGG; apa:APP7_0934; -.
DR   HOGENOM; CLU_178280_3_2_6; -.
DR   OMA; WAAEEHK; -.
DR   BioCyc; APLE537457:APP7_RS04750-MON; -.
DR   Proteomes; UP000001226; Chromosome.
DR   GO; GO:0008657; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) inhibitor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.50.10; -; 1.
DR   HAMAP; MF_00649; DNA_gyrase_inhibitor_YacG; 1.
DR   InterPro; IPR005584; DNA_gyrase_inhibitor_YacG.
DR   InterPro; IPR013088; Znf_NHR/GATA.
DR   PANTHER; PTHR36150; PTHR36150; 1.
DR   Pfam; PF03884; YacG; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Zinc.
FT   CHAIN           1..62
FT                   /note="DNA gyrase inhibitor YacG"
FT                   /id="PRO_1000130955"
FT   BINDING         8
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00649"
FT   BINDING         11
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00649"
FT   BINDING         27
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00649"
FT   BINDING         31
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00649"
SQ   SEQUENCE   62 AA;  7194 MW;  72F6863B261CFCCA CRC64;
     MSETIVNCPT CNQDVIWKPE SKYRPFCSER CQLIDLGEWA NEEKRIAAVE NDVMTSDLEG
     HY