YACG_ECOLI
ID YACG_ECOLI Reviewed; 65 AA.
AC P0A8H8; P36681; P75644; Q8KJQ7;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=DNA gyrase inhibitor YacG {ECO:0000255|HAMAP-Rule:MF_00649};
GN Name=yacG {ECO:0000255|HAMAP-Rule:MF_00649};
GN OrderedLocusNames=b0101, JW5008;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA Fujita N., Mori H., Yura T., Ishihama A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT 4.1 min (110,917-193,643 bp) region.";
RL Nucleic Acids Res. 22:1637-1639(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-65.
RC STRAIN=K12;
RX PubMed=3033442; DOI=10.1007/bf00326530;
RA Akiyama M., Horiuchi T., Sekiguchi M.;
RT "Molecular cloning and nucleotide sequence of the mutT mutator of
RT Escherichia coli that causes A:T to C:G transversion.";
RL Mol. Gen. Genet. 206:9-16(1987).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 34-65.
RC STRAIN=K12;
RX PubMed=1630901; DOI=10.1093/nar/20.13.3305;
RA Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K.,
RA Mizobuchi K., Nakata A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 0-
RT 2.4 min region.";
RL Nucleic Acids Res. 20:3305-3308(1992).
RN [6]
RP FUNCTION, AND INTERACTION WITH GYRB.
RX PubMed=18586829; DOI=10.1093/nar/gkn355;
RA Sengupta S., Nagaraja V.;
RT "YacG from Escherichia coli is a specific endogenous inhibitor of DNA
RT gyrase.";
RL Nucleic Acids Res. 36:4310-4316(2008).
RN [7]
RP STRUCTURE BY NMR.
RC STRAIN=K12;
RX PubMed=12211008; DOI=10.1002/prot.10214;
RA Ramelot T.A., Cort J.R., Yee A.A., Semesi A., Edwards A.M.,
RA Arrowsmith C.H., Kennedy M.A.;
RT "NMR structure of the Escherichia coli protein YacG: a novel sequence motif
RT in the zinc-finger family of proteins.";
RL Proteins 49:289-293(2002).
CC -!- FUNCTION: Inhibits all the catalytic activities of DNA gyrase by
CC preventing its interaction with DNA. Acts by binding directly to the C-
CC terminal domain of GyrB, which probably disrupts DNA binding by the
CC gyrase. {ECO:0000255|HAMAP-Rule:MF_00649, ECO:0000269|PubMed:18586829}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Note=Binds 1 zinc ion.;
CC -!- SUBUNIT: Interacts with GyrB. {ECO:0000255|HAMAP-Rule:MF_00649,
CC ECO:0000269|PubMed:18586829}.
CC -!- SIMILARITY: Belongs to the DNA gyrase inhibitor YacG family.
CC {ECO:0000255|HAMAP-Rule:MF_00649}.
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DR EMBL; U00096; AAC73212.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96668.2; -; Genomic_DNA.
DR EMBL; X04831; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; E64732; E64732.
DR RefSeq; NP_414643.1; NC_000913.3.
DR RefSeq; WP_000005042.1; NZ_STEB01000010.1.
DR PDB; 1LV3; NMR; -; A=1-65.
DR PDB; 4TMA; X-ray; 3.30 A; I/J/K/L=1-65.
DR PDBsum; 1LV3; -.
DR PDBsum; 4TMA; -.
DR AlphaFoldDB; P0A8H8; -.
DR SMR; P0A8H8; -.
DR BioGRID; 4261884; 1.
DR DIP; DIP-48126N; -.
DR IntAct; P0A8H8; 3.
DR STRING; 511145.b0101; -.
DR jPOST; P0A8H8; -.
DR PaxDb; P0A8H8; -.
DR PRIDE; P0A8H8; -.
DR DNASU; 945630; -.
DR EnsemblBacteria; AAC73212; AAC73212; b0101.
DR EnsemblBacteria; BAB96668; BAB96668; BAB96668.
DR GeneID; 66671610; -.
DR GeneID; 945630; -.
DR KEGG; ecj:JW5008; -.
DR KEGG; eco:b0101; -.
DR PATRIC; fig|1411691.4.peg.2180; -.
DR EchoBASE; EB2220; -.
DR eggNOG; COG3024; Bacteria.
DR HOGENOM; CLU_178280_3_1_6; -.
DR InParanoid; P0A8H8; -.
DR OMA; WAAEEHK; -.
DR PhylomeDB; P0A8H8; -.
DR BioCyc; EcoCyc:EG12314-MON; -.
DR EvolutionaryTrace; P0A8H8; -.
DR PRO; PR:P0A8H8; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0008657; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) inhibitor activity; IDA:EcoCyc.
DR GO; GO:0008270; F:zinc ion binding; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.30.50.10; -; 1.
DR HAMAP; MF_00649; DNA_gyrase_inhibitor_YacG; 1.
DR InterPro; IPR005584; DNA_gyrase_inhibitor_YacG.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR36150; PTHR36150; 1.
DR Pfam; PF03884; YacG; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Reference proteome; Zinc.
FT CHAIN 1..65
FT /note="DNA gyrase inhibitor YacG"
FT /id="PRO_0000211695"
FT REGION 45..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 9
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 12
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 28
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 32
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT STRAND 6..8
FT /evidence="ECO:0007829|PDB:4TMA"
FT TURN 10..12
FT /evidence="ECO:0007829|PDB:4TMA"
FT STRAND 15..19
FT /evidence="ECO:0007829|PDB:4TMA"
FT TURN 23..26
FT /evidence="ECO:0007829|PDB:4TMA"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:4TMA"
FT HELIX 30..41
FT /evidence="ECO:0007829|PDB:4TMA"
FT STRAND 42..46
FT /evidence="ECO:0007829|PDB:1LV3"
FT TURN 52..54
FT /evidence="ECO:0007829|PDB:1LV3"
FT TURN 62..64
FT /evidence="ECO:0007829|PDB:1LV3"
SQ SEQUENCE 65 AA; 7306 MW; F2747B62AFE6479D CRC64;
MSETITVNCP TCGKTVVWGE ISPFRPFCSK RCQLIDLGEW AAEEKRIPSS GDLSESDDWS
EEPKQ
