ID   YACL_BACSU              Reviewed;         366 AA.
AC   Q06754;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Uncharacterized PIN and TRAM-domain containing protein YacL;
DE   AltName: Full=Putative RNase YacL;
DE            EC=3.1.-.-;
GN   Name=yacL; OrderedLocusNames=BSU00890;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT   chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 223-366.
RX   PubMed=7510287; DOI=10.1016/s0021-9258(17)37310-6;
RA   Gagnon Y., Breton R., Putzer H., Pelchat M., Grunberg-Manago M.,
RA   Lapointe J.;
RT   "Clustering and co-transcription of the Bacillus subtilis genes encoding
RT   the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and
RT   the first enzyme for cysteine biosynthesis.";
RL   J. Biol. Chem. 269:7473-7482(1994).
RN   [4]
RP   INDUCTION.
RC   STRAIN=168 / 1604;
RX   PubMed=17434969; DOI=10.1128/jb.00130-07;
RA   Jervis A.J., Thackray P.D., Houston C.W., Horsburgh M.J., Moir A.;
RT   "SigM-responsive genes of Bacillus subtilis and their promoters.";
RL   J. Bacteriol. 189:4534-4538(2007).
CC   -!- FUNCTION: An RNase. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- INDUCTION: Transcribed under partial control of SigM ECF sigma factor
CC       (PubMed:17434969). {ECO:0000269|PubMed:17434969}.
CC   -!- SIMILARITY: Belongs to the ycf81 family. {ECO:0000305}.
CC   -!- SIMILARITY: In the central section; belongs to the PINc/VapC protein
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D26185; BAA05323.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11865.1; -; Genomic_DNA.
DR   EMBL; L14580; AAA21793.1; -; Genomic_DNA.
DR   PIR; S66118; S66118.
DR   RefSeq; NP_387970.1; NC_000964.3.
DR   RefSeq; WP_003235014.1; NZ_JNCM01000029.1.
DR   AlphaFoldDB; Q06754; -.
DR   SMR; Q06754; -.
DR   IntAct; Q06754; 1.
DR   STRING; 224308.BSU00890; -.
DR   PaxDb; Q06754; -.
DR   PRIDE; Q06754; -.
DR   EnsemblBacteria; CAB11865; CAB11865; BSU_00890.
DR   GeneID; 936863; -.
DR   KEGG; bsu:BSU00890; -.
DR   PATRIC; fig|224308.179.peg.90; -.
DR   eggNOG; COG4956; Bacteria.
DR   InParanoid; Q06754; -.
DR   OMA; TMPTDLL; -.
DR   PhylomeDB; Q06754; -.
DR   BioCyc; BSUB:BSU00890-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR002792; TRAM_dom.
DR   SMART; SM00670; PINc; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   PROSITE; PS50926; TRAM; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..366
FT                   /note="Uncharacterized PIN and TRAM-domain containing
FT                   protein YacL"
FT                   /id="PRO_0000217410"
FT   DOMAIN          169..280
FT                   /note="PINc"
FT   DOMAIN          295..356
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   BINDING         250
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   366 AA;  40858 MW;  19D8483FF0F74D5F CRC64;
     MLKRIVQAFF IIFGGVVGIF LIPELFVLLN IQDIPLITNA YTSAAIGAII FFLISIWGTE
     YVVNWVKWIE DSLLKAPVPD LLFGSLGLVF GLIIAYLIVN VIPLDNIPYR IFSTIIPVFL
     AFFLGYLGFQ VGFKKKDELI SLFSISARMQ KKKGTADEEH EVQDKKLKIL DTSVIIDGRI
     ADICQTGFLE GVIVIPQFVL EELQHIADSS DVLKRNRGRR GLDILNRIQK ELDIEVEIYE
     GDFEDIQEVD SKLVKLAKLT SGVVVTNDFN LNKVCELQKV AVLNINDLAN AVKPVVLPGE
     EMNVQVIKDG KEHNQGVAYL DDGTMIVVEE GRNYIGKHID VLVTSVLQTA AGRMIFAKPK
     LLEKAL