ID   YADI_ECOLI              Reviewed;         146 AA.
AC   P36881; P75658;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 145.
DE   RecName: Full=Putative phosphotransferase enzyme IIA component YadI;
DE   AltName: Full=Putative PTS system EIIA component;
GN   Name=yadI; OrderedLocusNames=b0129, JW0125;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA   Fujita N., Mori H., Yura T., Ishihama A.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT   4.1 min (110,917-193,643 bp) region.";
RL   Nucleic Acids Res. 22:1637-1639(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC       system (sugar PTS), a major carbohydrate active -transport system,
CC       catalyzes the phosphorylation of incoming sugar substrates
CC       concomitantly with their translocation across the cell membrane.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The EIIA domain is phosphorylated by phospho-HPr on a histidyl
CC       residue. Then, it transfers the phosphoryl group to the EIIB domain.
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DR   EMBL; U00096; AAC73240.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAB96706.2; -; Genomic_DNA.
DR   PIR; A64736; A64736.
DR   RefSeq; NP_414671.1; NC_000913.3.
DR   RefSeq; WP_000901987.1; NZ_STEB01000010.1.
DR   AlphaFoldDB; P36881; -.
DR   SMR; P36881; -.
DR   STRING; 511145.b0129; -.
DR   PaxDb; P36881; -.
DR   PRIDE; P36881; -.
DR   DNASU; 947397; -.
DR   EnsemblBacteria; AAC73240; AAC73240; b0129.
DR   EnsemblBacteria; BAB96706; BAB96706; BAB96706.
DR   GeneID; 947397; -.
DR   KEGG; ecj:JW0125; -.
DR   KEGG; eco:b0129; -.
DR   PATRIC; fig|1411691.4.peg.2153; -.
DR   EchoBASE; EB2227; -.
DR   eggNOG; COG2893; Bacteria.
DR   HOGENOM; CLU_123235_1_0_6; -.
DR   InParanoid; P36881; -.
DR   OMA; GWVIACH; -.
DR   PhylomeDB; P36881; -.
DR   BioCyc; EcoCyc:AGAX-MON; -.
DR   BioCyc; MetaCyc:AGAX-MON; -.
DR   PRO; PR:P36881; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:InterPro.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd00006; PTS_IIA_man; 1.
DR   Gene3D; 3.40.50.510; -; 1.
DR   InterPro; IPR004701; PTS_EIIA_man-typ.
DR   InterPro; IPR036662; PTS_EIIA_man-typ_sf.
DR   InterPro; IPR033887; PTS_IIA_man.
DR   Pfam; PF03610; EIIA-man; 1.
DR   SUPFAM; SSF53062; SSF53062; 1.
DR   PROSITE; PS51096; PTS_EIIA_TYPE_4; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Phosphotransferase system; Reference proteome;
KW   Sugar transport; Transferase; Transport.
FT   CHAIN           1..146
FT                   /note="Putative phosphotransferase enzyme IIA component
FT                   YadI"
FT                   /id="PRO_0000186708"
FT   DOMAIN          1..124
FT                   /note="PTS EIIA type-4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00419"
FT   ACT_SITE        9
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00419"
SQ   SEQUENCE   146 AA;  16540 MW;  9534A95A41130CE5 CRC64;
     MLGWVITCHD DRAQEILDAL EKKHGALLQC RAVNFWRGLS SNMLSRMMCD ALHEADSGEG
     VIFLTDIAGA PPYRVASLLS HKHSRCEVIS GVTLPLIEQM MACRETMTSS EFRERIVELG
     APEVSSLWHQ QQKNPPFVLK HNLYEY