YAE1_YEAST
ID YAE1_YEAST Reviewed; 141 AA.
AC P47118; D6VWN8;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Protein YAE1;
GN Name=YAE1; OrderedLocusNames=YJR067C; ORFNames=J1805;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8840504;
RX DOI=10.1002/(sici)1097-0061(199607)12:9<869::aid-yea964>3.0.co;2-1;
RA Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
RT "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading
RT frames and a gene cluster with a counterpart on chromosome XI.";
RL Yeast 12:869-875(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP INTERACTION WITH LTO1 AND RLI1.
RX PubMed=23318452; DOI=10.1038/onc.2012.604;
RA Zhai C., Li Y., Mascarenhas C., Lin Q., Li K., Vyrides I., Grant C.M.,
RA Panaretou B.;
RT "The function of ORAOV1/LTO1, a gene that is overexpressed frequently in
RT cancer: essential roles in the function and biogenesis of the ribosome.";
RL Oncogene 33:484-494(2014).
RN [9]
RP FUNCTION, AND INTERACTION WITH LTO1 AND RLI1.
RX PubMed=26182403; DOI=10.7554/elife.08231;
RA Paul V.D., Muehlenhoff U., Stuempfig M., Seebacher J., Kugler K.G.,
RA Renicke C., Taxis C., Gavin A.C., Pierik A.J., Lill R.;
RT "The deca-GX3 proteins Yae1-Lto1 function as adaptors recruiting the ABC
RT protein Rli1 for iron-sulfur cluster insertion.";
RL Elife 4:E08231-E08231(2015).
CC -!- FUNCTION: The complex LTO1:YAE1 functions as a target specific adapter
CC that recruits apo-RLI1 to the cytosolic iron-sulfur protein assembly
CC (CIA) complex machinery. {ECO:0000269|PubMed:26182403}.
CC -!- SUBUNIT: Forms a complex with LTO1; the complex bridges the interaction
CC between the CIA complex and RLI1 (PubMed:23318452, PubMed:26182403).
CC Interacts with RLI1 (PubMed:23318452, PubMed:26182403).
CC {ECO:0000269|PubMed:23318452, ECO:0000269|PubMed:26182403}.
CC -!- INTERACTION:
CC P47118; P53846: LTO1; NbExp=2; IntAct=EBI-25522, EBI-28226;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1500 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the YAE1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z49567; CAA89595.1; -; Genomic_DNA.
DR EMBL; L47993; AAB39293.1; -; Genomic_DNA.
DR EMBL; AY557892; AAS56218.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08854.1; -; Genomic_DNA.
DR PIR; S57086; S57086.
DR RefSeq; NP_012601.1; NM_001181725.1.
DR AlphaFoldDB; P47118; -.
DR SMR; P47118; -.
DR BioGRID; 33824; 54.
DR DIP; DIP-5706N; -.
DR IntAct; P47118; 1.
DR MINT; P47118; -.
DR STRING; 4932.YJR067C; -.
DR iPTMnet; P47118; -.
DR MaxQB; P47118; -.
DR PaxDb; P47118; -.
DR PRIDE; P47118; -.
DR EnsemblFungi; YJR067C_mRNA; YJR067C; YJR067C.
DR GeneID; 853530; -.
DR KEGG; sce:YJR067C; -.
DR SGD; S000003828; YAE1.
DR VEuPathDB; FungiDB:YJR067C; -.
DR eggNOG; KOG4774; Eukaryota.
DR HOGENOM; CLU_066684_2_0_1; -.
DR InParanoid; P47118; -.
DR OMA; DESYHEL; -.
DR BioCyc; YEAST:G3O-31700-MON; -.
DR PRO; PR:P47118; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47118; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0062092; C:Yae1-Lto1 complex; IDA:SGD.
DR GO; GO:0106035; P:protein maturation by [4Fe-4S] cluster transfer; IDA:UniProtKB.
DR InterPro; IPR019191; Essential_protein_Yae1_N.
DR InterPro; IPR038881; Yae1-like.
DR PANTHER; PTHR18829; PTHR18829; 1.
DR Pfam; PF09811; Yae1_N; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..141
FT /note="Protein YAE1"
FT /id="PRO_0000203099"
FT REGION 34..74
FT /note="deca-GX3 motif; required for interaction with LTO1"
FT /evidence="ECO:0000269|PubMed:26182403"
SQ SEQUENCE 141 AA; 16076 MW; AD0D90D51C03C996 CRC64;
MSNTWDDVWA SDSDVETERS PDLVKLRENH SKRGYLDGIV SSKEEKLQEG FNDGFPTGAK
LGKQVGIIMG ILLGLRTRFG DEDEDLSKAY IDAQKELRIN KVLSKSIFDP NFDLQEKHPL
ITKWTDIANT YCEKYHVPSI Q
