YAEC_SCHPO
ID YAEC_SCHPO Reviewed; 559 AA.
AC Q09852; Q9USC7;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Putative inorganic phosphate transporter C23D3.12;
GN ORFNames=SPAC23D3.12;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 217-411, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297 AND SER-299, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10759889}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:10759889}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; CU329670; CAA91247.1; -; Genomic_DNA.
DR EMBL; AB027861; BAA87165.1; -; Genomic_DNA.
DR PIR; T38287; S62503.
DR RefSeq; NP_594549.1; NM_001019978.2.
DR AlphaFoldDB; Q09852; -.
DR SMR; Q09852; -.
DR BioGRID; 277986; 6.
DR STRING; 4896.SPAC23D3.12.1; -.
DR TCDB; 2.A.1.9.8; the major facilitator superfamily (mfs).
DR iPTMnet; Q09852; -.
DR MaxQB; Q09852; -.
DR PaxDb; Q09852; -.
DR PRIDE; Q09852; -.
DR EnsemblFungi; SPAC23D3.12.1; SPAC23D3.12.1:pep; SPAC23D3.12.
DR GeneID; 2541484; -.
DR KEGG; spo:SPAC23D3.12; -.
DR PomBase; SPAC23D3.12; -.
DR VEuPathDB; FungiDB:SPAC23D3.12; -.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q09852; -.
DR OMA; HWTAKGN; -.
DR PhylomeDB; Q09852; -.
DR PRO; PR:Q09852; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0071944; C:cell periphery; IDA:PomBase.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:PomBase.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; ISO:PomBase.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; ISO:PomBase.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphate transport; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..559
FT /note="Putative inorganic phosphate transporter C23D3.12"
FT /id="PRO_0000050478"
FT TOPO_DOM 1..45
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..94
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..118
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..144
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..224
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..335
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 357..381
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 403..409
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 431..438
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..474
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 496..497
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 498..518
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 519..559
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 297
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 559 AA; 62316 MW; 2E8958F86C2092E2 CRC64;
MNRLNPFSKS HSKNSNENNE VSLADVAESD TRRHWLGLTK REFKLMGFAG AGFFLDSYDL
FIINLVSPIY EYLYWGGLEG KKPHYPSGIH GLVNAAANIG NVFGQILFGF MGDFFGRKFV
YGKEMIVVII ATVLVIALPK SIPTPLGKMM WIFAWRWLLG LGIGGDYPMS ATITSERSLL
SRRGTLLSIV FSFQGFGTLA GAIVTIILLA CFEKPLNQRG EYTKLEGVWR LQMGLALVPA
LLVLIPRLTM KESKSYEQSK ALNKYTDNDT YIADDDEPKK DNQNVVEEKQ INLTTSSDSH
PTSTEDFGDK RASTVPTSEN TSGFIEYFSQ WHHFKHLLAT AVSWFLLDIA FYGVNLNQSV
ILKAIGFSSG KNEYHTLMRG AIGNLIIAIA GYVPGYWFTV FLVEKLGRKW IQLQGLFITG
LMFAILAGSW DTISTGGRFA CFVIAQFFSN FGPNATTFLY PAEVFPARVR GTAHGLSAAL
GKCGAILASL LFNFLTSVIG YGNVMWIFCG CMWGAIFFTL LLPETKMRDA DEIDREEVLR
GGNGKTHQGR WSWYFNGIF
