ID   YAEF_ECOLI              Reviewed;         274 AA.
AC   P37056;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 3.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=Probable lipoprotein peptidase YaeF;
DE            EC=3.4.-.-;
DE   AltName: Full=Uncharacterized lipoprotein YaeF;
DE   Flags: Precursor;
GN   Name=yaeF; Synonyms=yaeK; OrderedLocusNames=b0193, JW5016;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=7635807; DOI=10.1128/jb.177.15.4207-4215.1995;
RA   Gupta S.D., Lee B.T.O., Camakaris J., Wu H.C.;
RT   "Identification of cutC and cutF (nlpE) genes involved in copper tolerance
RT   in Escherichia coli.";
RL   J. Bacteriol. 177:4207-4215(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Yamamoto Y.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-144.
RX   PubMed=2203971; DOI=10.1038/347203a0;
RA   Eriani G., Delarue M., Poch O., Gangloff J., Moras D.;
RT   "Partition of tRNA synthetases into two classes based on mutually exclusive
RT   sets of sequence motifs.";
RL   Nature 347:203-206(1990).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-126.
RX   PubMed=1688424; DOI=10.1128/jb.172.1.281-286.1990;
RA   Zhou Z., Syvanen M.;
RT   "Identification and sequence of the drpA gene from Escherichia coli.";
RL   J. Bacteriol. 172:281-286(1990).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-274.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=7635808; DOI=10.1128/jb.177.15.4216-4223.1995;
RA   Snyder W.B., Davis L.J., Danese P.N., Cosma C.L., Silhavy T.J.;
RT   "Overproduction of NlpE, a new outer membrane lipoprotein, suppresses the
RT   toxicity of periplasmic LacZ by activation of the Cpx signal transduction
RT   pathway.";
RL   J. Bacteriol. 177:4216-4223(1995).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA82973.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAB08621.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAA08434.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; L38619; AAA82973.1; ALT_INIT; Genomic_DNA.
DR   EMBL; D49445; BAA08434.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U70214; AAB08621.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC73304.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77869.2; -; Genomic_DNA.
DR   EMBL; M97858; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; M32357; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U18345; AAA86094.1; -; Genomic_DNA.
DR   PIR; A64744; A64744.
DR   RefSeq; NP_414735.4; NC_000913.3.
DR   RefSeq; WP_001336393.1; NZ_SSZK01000004.1.
DR   AlphaFoldDB; P37056; -.
DR   SMR; P37056; -.
DR   BioGRID; 4261951; 286.
DR   STRING; 511145.b0193; -.
DR   PaxDb; P37056; -.
DR   PRIDE; P37056; -.
DR   EnsemblBacteria; AAC73304; AAC73304; b0193.
DR   EnsemblBacteria; BAA77869; BAA77869; BAA77869.
DR   GeneID; 944892; -.
DR   KEGG; ecj:JW5016; -.
DR   KEGG; eco:b0193; -.
DR   PATRIC; fig|1411691.4.peg.2085; -.
DR   EchoBASE; EB2059; -.
DR   eggNOG; COG0791; Bacteria.
DR   HOGENOM; CLU_093835_0_0_6; -.
DR   InParanoid; P37056; -.
DR   OMA; AWFCSEF; -.
DR   BioCyc; EcoCyc:EG12138-MON; -.
DR   PRO; PR:P37056; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR024453; Peptidase_C92.
DR   Pfam; PF05708; Peptidase_C92; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Lipoprotein; Membrane;
KW   Palmitate; Protease; Reference proteome; Signal; Thiol protease.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..274
FT                   /note="Probable lipoprotein peptidase YaeF"
FT                   /id="PRO_0000019766"
FT   ACT_SITE        207
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        257
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   274 AA;  29912 MW;  2E9496BB02148943 CRC64;
     MDKPKAYCRL FLPSFLLLSA CTVDISQPDP SATAVDAEAK TWAVKFQHQS SFTEQSIKEI
     TAPDLKPGDL LFSSSLGVTS FGIRVFSTSS VSHVAIFLGD NNVAEATGAG VQIVSLKKAM
     KHSDKLFVLR VPDLTPQQAT DITAFANKIK DSGYNYRGIV EFIPFMVTRQ MCSLNPFSED
     FRQQCVSGLA KAQLSSVGEG DKKSWFCSEF VTDAFAKAGH PLTLAQSGWI SPADLMHMRI
     GDVSAFKPET QLQYVGHLKP GIYIKAGRFV GLTR