ID   YAFJ_ECOLI              Reviewed;         255 AA.
AC   Q47147;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Putative glutamine amidotransferase YafJ;
DE            EC=2.4.2.-;
GN   Name=yafJ; OrderedLocusNames=b0223, JW0213;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=7596361; DOI=10.1016/0165-7992(95)90024-1;
RA   Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R.;
RT   "dinP, a new gene in Escherichia coli, whose product shows similarities to
RT   UmuC and its homologues.";
RL   Mutat. Res. 347:1-7(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- INTERACTION:
CC       Q47147; P12996: bioB; NbExp=4; IntAct=EBI-1114805, EBI-557917;
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DR   EMBL; D38582; BAA07585.1; -; Genomic_DNA.
DR   EMBL; U70214; AAB08645.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73327.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77893.1; -; Genomic_DNA.
DR   PIR; H64746; H64746.
DR   RefSeq; NP_414758.1; NC_000913.3.
DR   RefSeq; WP_000333380.1; NZ_STEB01000020.1.
DR   AlphaFoldDB; Q47147; -.
DR   SMR; Q47147; -.
DR   BioGRID; 4260847; 12.
DR   BioGRID; 849305; 10.
DR   IntAct; Q47147; 14.
DR   STRING; 511145.b0223; -.
DR   jPOST; Q47147; -.
DR   PaxDb; Q47147; -.
DR   PRIDE; Q47147; -.
DR   EnsemblBacteria; AAC73327; AAC73327; b0223.
DR   EnsemblBacteria; BAA77893; BAA77893; BAA77893.
DR   GeneID; 66671458; -.
DR   GeneID; 944906; -.
DR   KEGG; ecj:JW0213; -.
DR   KEGG; eco:b0223; -.
DR   PATRIC; fig|1411691.4.peg.2060; -.
DR   EchoBASE; EB2941; -.
DR   eggNOG; COG0121; Bacteria.
DR   HOGENOM; CLU_059273_0_0_6; -.
DR   InParanoid; Q47147; -.
DR   OMA; YWVFAHN; -.
DR   PhylomeDB; Q47147; -.
DR   BioCyc; EcoCyc:G6107-MON; -.
DR   PRO; PR:Q47147; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR026869; EgtC-like.
DR   InterPro; IPR017932; GATase_2_dom.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   Pfam; PF13230; GATase_4; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
DR   PROSITE; PS51278; GATASE_TYPE_2; 1.
PE   1: Evidence at protein level;
KW   Glutamine amidotransferase; Reference proteome; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..255
FT                   /note="Putative glutamine amidotransferase YafJ"
FT                   /id="PRO_0000168534"
FT   DOMAIN          2..251
FT                   /note="Glutamine amidotransferase type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT   ACT_SITE        2
FT                   /note="For GATase activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   255 AA;  28636 MW;  EEC155D4B833A0A1 CRC64;
     MCELLGMSAN VPTDICFSFT GLVQRGGGTG PHKDGWGITF YEGKGCRTFK DPQPSFNSPI
     AKLVQDYPIK SCSVVAHIRQ ANRGEVALEN THPFTRELWG RNWTYAHNGQ LTGYKSLETG
     NFRPVGETDS EKAFCWLLHK LTQRYPRTPG NMAAVFKYIA SLADELRQKG VFNMLLSDGR
     YVMAYCSTNL HWITRRAPFG VATLLDQDVE IDFSSQTTPN DVVTVIATQP LTGNETWQKI
     MPGEWRLFCL GERVV