ID   YAFK_ECO57              Reviewed;         246 AA.
AC   P0AAA0; Q47148;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Putative L,D-transpeptidase YafK;
DE            EC=2.-.-.-;
DE   Flags: Precursor;
GN   Name=yafK; OrderedLocusNames=Z0282, ECs0251;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SIMILARITY: Belongs to the YkuD family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG54549.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB33674.1; -; Genomic_DNA.
DR   PIR; A85511; A85511.
DR   PIR; C90660; C90660.
DR   RefSeq; NP_308278.1; NC_002695.1.
DR   RefSeq; WP_001225679.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AAA0; -.
DR   SMR; P0AAA0; -.
DR   STRING; 155864.EDL933_0254; -.
DR   MEROPS; C82.A01; -.
DR   EnsemblBacteria; AAG54549; AAG54549; Z0282.
DR   EnsemblBacteria; BAB33674; BAB33674; ECs_0251.
DR   GeneID; 66671457; -.
DR   GeneID; 914338; -.
DR   KEGG; ece:Z0282; -.
DR   KEGG; ecs:ECs_0251; -.
DR   PATRIC; fig|386585.9.peg.351; -.
DR   eggNOG; COG3034; Bacteria.
DR   HOGENOM; CLU_032558_2_0_6; -.
DR   OMA; MAQIYAF; -.
DR   UniPathway; UPA00219; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071972; F:peptidoglycan L,D-transpeptidase activity; IEA:UniProt.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   CDD; cd16913; YkuD_like; 1.
DR   Gene3D; 2.40.440.10; -; 1.
DR   InterPro; IPR005490; LD_TPept_cat_dom.
DR   InterPro; IPR038063; Transpep_catalytic_dom.
DR   Pfam; PF03734; YkuD; 1.
DR   SUPFAM; SSF141523; SSF141523; 1.
PE   3: Inferred from homology;
KW   Cell shape; Cell wall biogenesis/degradation; Glycosyltransferase;
KW   Hydrolase; Peptidoglycan synthesis; Reference proteome; Signal;
KW   Transferase.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..246
FT                   /note="Putative L,D-transpeptidase YafK"
FT                   /id="PRO_0000041955"
SQ   SEQUENCE   246 AA;  28021 MW;  D2B6686353D9735A CRC64;
     MRKIALILAM LLIPCVSFAG LLGSSSSTTP VSKEYKQQLM GSPVYIQIFK EERTLDLYVK
     MGEQYQLLDS YKICKYSGGL GPKQRQGDFK SPEGFYSVQR NQLKPDSRYY KAINIGFPNA
     YDRAHGYEGK YLMIHGDCVS IGCYAMTNQG IDEIFQFVTG ALVFGQPSVQ VSIYPFRMTD
     ANMKRHKYSN FKDFWEQLKP GYDYFEQTRK PPTVSVVNGR YVVSKPLSHE VVQPQLASNY
     TLPEAK