ID   YAFK_ECOLI              Reviewed;         246 AA.
AC   P0AA99; Q47148;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Putative L,D-transpeptidase YafK;
DE            EC=2.-.-.-;
DE   Flags: Precursor;
GN   Name=yafK; OrderedLocusNames=b0224, JW0214;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=7596361; DOI=10.1016/0165-7992(95)90024-1;
RA   Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R.;
RT   "dinP, a new gene in Escherichia coli, whose product shows similarities to
RT   UmuC and its homologues.";
RL   Mutat. Res. 347:1-7(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SIMILARITY: Belongs to the YkuD family. {ECO:0000305}.
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DR   EMBL; D38582; BAA07586.1; -; Genomic_DNA.
DR   EMBL; U70214; AAB08646.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73328.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77894.1; -; Genomic_DNA.
DR   PIR; A64747; A64747.
DR   RefSeq; NP_414759.1; NC_000913.3.
DR   RefSeq; WP_001225679.1; NZ_STEB01000020.1.
DR   AlphaFoldDB; P0AA99; -.
DR   SMR; P0AA99; -.
DR   BioGRID; 4262122; 20.
DR   STRING; 511145.b0224; -.
DR   MEROPS; C82.A01; -.
DR   PaxDb; P0AA99; -.
DR   PRIDE; P0AA99; -.
DR   EnsemblBacteria; AAC73328; AAC73328; b0224.
DR   EnsemblBacteria; BAA77894; BAA77894; BAA77894.
DR   GeneID; 66671457; -.
DR   GeneID; 944910; -.
DR   KEGG; ecj:JW0214; -.
DR   KEGG; eco:b0224; -.
DR   PATRIC; fig|511145.12.peg.226; -.
DR   EchoBASE; EB2942; -.
DR   eggNOG; COG3034; Bacteria.
DR   HOGENOM; CLU_032558_2_0_6; -.
DR   InParanoid; P0AA99; -.
DR   OMA; MAQIYAF; -.
DR   PhylomeDB; P0AA99; -.
DR   BioCyc; EcoCyc:G6108-MON; -.
DR   BioCyc; MetaCyc:G6108-MON; -.
DR   UniPathway; UPA00219; -.
DR   PRO; PR:P0AA99; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071972; F:peptidoglycan L,D-transpeptidase activity; IEA:UniProt.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   CDD; cd16913; YkuD_like; 1.
DR   Gene3D; 2.40.440.10; -; 1.
DR   InterPro; IPR005490; LD_TPept_cat_dom.
DR   InterPro; IPR038063; Transpep_catalytic_dom.
DR   Pfam; PF03734; YkuD; 1.
DR   SUPFAM; SSF141523; SSF141523; 1.
PE   3: Inferred from homology;
KW   Cell shape; Cell wall biogenesis/degradation; Glycosyltransferase;
KW   Hydrolase; Peptidoglycan synthesis; Reference proteome; Signal;
KW   Transferase.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..246
FT                   /note="Putative L,D-transpeptidase YafK"
FT                   /id="PRO_0000013784"
SQ   SEQUENCE   246 AA;  28021 MW;  D2B6686353D9735A CRC64;
     MRKIALILAM LLIPCVSFAG LLGSSSSTTP VSKEYKQQLM GSPVYIQIFK EERTLDLYVK
     MGEQYQLLDS YKICKYSGGL GPKQRQGDFK SPEGFYSVQR NQLKPDSRYY KAINIGFPNA
     YDRAHGYEGK YLMIHGDCVS IGCYAMTNQG IDEIFQFVTG ALVFGQPSVQ VSIYPFRMTD
     ANMKRHKYSN FKDFWEQLKP GYDYFEQTRK PPTVSVVNGR YVVSKPLSHE VVQPQLASNY
     TLPEAK