YAFL_ECOLI
ID YAFL_ECOLI Reviewed; 249 AA.
AC Q47151; Q9R2E0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Probable endopeptidase YafL;
DE EC=3.4.-.-;
DE AltName: Full=Uncharacterized lipoprotein YafL;
DE Flags: Precursor;
GN Name=yafL; OrderedLocusNames=b0227, JW0217;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7596361; DOI=10.1016/0165-7992(95)90024-1;
RA Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R.;
RT "dinP, a new gene in Escherichia coli, whose product shows similarities to
RT UmuC and its homologues.";
RL Mutat. Res. 347:1-7(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT - 6.0 min (189,987 - 281,416bp) region.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase C40 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01284, ECO:0000305}.
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DR EMBL; D38582; BAA07589.1; -; Genomic_DNA.
DR EMBL; U70214; AAB08647.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73331.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA77897.2; -; Genomic_DNA.
DR PIR; D64747; D64747.
DR RefSeq; NP_414762.1; NC_000913.3.
DR RefSeq; WP_000056849.1; NZ_SSZK01000029.1.
DR AlphaFoldDB; Q47151; -.
DR SMR; Q47151; -.
DR BioGRID; 4259761; 264.
DR IntAct; Q47151; 6.
DR STRING; 511145.b0227; -.
DR PaxDb; Q47151; -.
DR PRIDE; Q47151; -.
DR EnsemblBacteria; AAC73331; AAC73331; b0227.
DR EnsemblBacteria; BAA77897; BAA77897; BAA77897.
DR GeneID; 944899; -.
DR KEGG; ecj:JW0217; -.
DR KEGG; eco:b0227; -.
DR PATRIC; fig|511145.12.peg.229; -.
DR EchoBASE; EB2943; -.
DR eggNOG; COG0791; Bacteria.
DR HOGENOM; CLU_016043_2_1_6; -.
DR InParanoid; Q47151; -.
DR OMA; RYGPGYT; -.
DR PhylomeDB; Q47151; -.
DR BioCyc; EcoCyc:G6111-MON; -.
DR PRO; PR:Q47151; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR000064; NLP_P60_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR Pfam; PF00877; NLPC_P60; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS51935; NLPC_P60; 1.
PE 3: Inferred from homology;
KW Cell membrane; Hydrolase; Lipoprotein; Membrane; Palmitate; Protease;
KW Reference proteome; Signal; Thiol protease.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..249
FT /note="Probable endopeptidase YafL"
FT /id="PRO_0000019767"
FT DOMAIN 116..243
FT /note="NlpC/P60"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 147
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 202
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 214
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT LIPID 18
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 18
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 249 AA; 28785 MW; 3C4F4618212C3466 CRC64;
MSLPSIPSFV LSGLLLICLP FSSFASATTS HISFSYAARQ RMQNRARLLK QYQTHLKKQA
SYIVEGNAES KRALRQHNRE QIKQHPEWFP APLKASDRRW QALAENNHFL SSDHLHNITE
VAIHRLEQQL GKPYVWGGTR PDKGFDCSGL VFYAYNKILE AKLPRTANEM YHYRRATIVA
NNDLRRGDLL FFHIHSREIA DHMGVYLGDG QFIESPRTGE TIRISRLAEP FWQDHFLGAR
RILTEETIL
