YAFN_ECOLI
ID YAFN_ECOLI Reviewed; 97 AA.
AC Q47156; Q2MCF9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Antitoxin YafN;
GN Name=yafN; OrderedLocusNames=b0232, JW0222;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7596361; DOI=10.1016/0165-7992(95)90024-1;
RA Ohmori H., Hatada E., Qiao Y., Tsuji M., Fukuda R.;
RT "dinP, a new gene in Escherichia coli, whose product shows similarities to
RT UmuC and its homologues.";
RL Mutat. Res. 347:1-7(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUGGESTION OF ANTITOXIN FUNCTION, INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12813093; DOI=10.1128/jb.185.13.3972-3977.2003;
RA McKenzie G.J., Magner D.B., Lee P.L., Rosenberg S.M.;
RT "The dinB operon and spontaneous mutation in Escherichia coli.";
RL J. Bacteriol. 185:3972-3977(2003).
RN [6]
RP FUNCTION AS AN MRNA INTERFERASE ANTITOXIN.
RC STRAIN=K12 / BW25113;
RX PubMed=19617347; DOI=10.1074/jbc.m109.036624;
RA Zhang Y., Yamaguchi Y., Inouye M.;
RT "Characterization of YafO, an Escherichia coli toxin.";
RL J. Biol. Chem. 284:25522-25531(2009).
RN [7]
RP FUNCTION AS AN MRNA INTERFERASE ANTITOXIN, INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=19943910; DOI=10.1111/j.1365-2958.2009.06969.x;
RA Christensen-Dalsgaard M., Jorgensen M.G., Gerdes K.;
RT "Three new RelE-homologous mRNA interferases of Escherichia coli
RT differentially induced by environmental stresses.";
RL Mol. Microbiol. 75:333-348(2010).
CC -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC Functions as an mRNA interferase antitoxin; overexpression prevents
CC YafO-mediated cessation of cell growth and inhibition of cell
CC proliferation. {ECO:0000269|PubMed:19617347,
CC ECO:0000269|PubMed:19943910}.
CC -!- SUBUNIT: Probably forms a complex with the mRNA interferase YafO which
CC inhibits the mRNA interferase activity. {ECO:0000305}.
CC -!- INTERACTION:
CC Q47156; Q47157: yafO; NbExp=2; IntAct=EBI-1114856, EBI-1119355;
CC -!- INDUCTION: Induced by amino acid starvation, glucose starvation, the
CC DNA cross-linker mitomycin C (SOS response) and when translation is
CC blocked. Induction is decreased in the absence of the Lon protease
CC suggesting, by homology to other toxin-antitoxin systems, that Lon may
CC degrade the YafN antitoxin. Transcription is negatively regulated by
CC the cognate locus, probably by this protein. A member of the dinB-
CC yafNOP operon; it has 2 promoters, 1 upstream of dinB and 1 specific
CC for yafN-yfaO. {ECO:0000269|PubMed:12813093,
CC ECO:0000269|PubMed:19943910}.
CC -!- SIMILARITY: Belongs to the phD/YefM antitoxin family. {ECO:0000305}.
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DR EMBL; D38582; BAA07594.1; -; Genomic_DNA.
DR EMBL; U70214; AAB08652.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73336.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76047.1; -; Genomic_DNA.
DR PIR; A64748; A64748.
DR RefSeq; NP_414767.1; NC_000913.3.
DR RefSeq; WP_000554758.1; NZ_SSZK01000050.1.
DR AlphaFoldDB; Q47156; -.
DR BioGRID; 4259762; 73.
DR ComplexPortal; CPX-6026; YafNO toxin-antitoxin complex.
DR IntAct; Q47156; 3.
DR STRING; 511145.b0232; -.
DR PaxDb; Q47156; -.
DR PRIDE; Q47156; -.
DR EnsemblBacteria; AAC73336; AAC73336; b0232.
DR EnsemblBacteria; BAE76047; BAE76047; BAE76047.
DR GeneID; 67414504; -.
DR GeneID; 944920; -.
DR KEGG; ecj:JW0222; -.
DR KEGG; eco:b0232; -.
DR PATRIC; fig|1411691.4.peg.2051; -.
DR EchoBASE; EB2945; -.
DR eggNOG; COG2161; Bacteria.
DR HOGENOM; CLU_2391108_0_0_6; -.
DR InParanoid; Q47156; -.
DR OMA; NNKPAGY; -.
DR PhylomeDB; Q47156; -.
DR BioCyc; EcoCyc:G6116-MON; -.
DR PRO; PR:Q47156; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0044092; P:negative regulation of molecular function; IDA:EcoCyc.
DR GO; GO:0040008; P:regulation of growth; IC:ComplexPortal.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR GO; GO:0009432; P:SOS response; IC:ComplexPortal.
DR InterPro; IPR036165; YefM-like_sf.
DR SUPFAM; SSF143120; SSF143120; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; DNA-binding; Reference proteome; Repressor;
KW SOS response; Toxin-antitoxin system; Transcription;
KW Transcription regulation.
FT CHAIN 1..97
FT /note="Antitoxin YafN"
FT /id="PRO_0000168538"
SQ SEQUENCE 97 AA; 11234 MW; C49811AE1E90A659 CRC64;
MHRILAEKSV NITELRKNPA KYFIDQPVAV LSNNRPAGYL LSASAFEALM DMLAEQEEKK
PIKARFRPSA ARLEEITRRA EQYLNDMTDD DFNDFKE
