YAFO_ECOLI
ID YAFO_ECOLI Reviewed; 132 AA.
AC Q47157;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=mRNA interferase toxin YafO;
DE EC=3.1.-.-;
DE AltName: Full=Endoribonuclease YafO;
DE AltName: Full=Toxin YafO;
GN Name=yafO; OrderedLocusNames=b0233, JW0223;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Ohmori H.;
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT - 6.0 min (189,987 - 281,416bp) region.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=12813093; DOI=10.1128/jb.185.13.3972-3977.2003;
RA McKenzie G.J., Magner D.B., Lee P.L., Rosenberg S.M.;
RT "The dinB operon and spontaneous mutation in Escherichia coli.";
RL J. Bacteriol. 185:3972-3977(2003).
RN [7]
RP FUNCTION AS AN MRNA INTERFERASE, AND LARGE RIBOSOMAL SUBUNIT-BINDING.
RC STRAIN=K12 / BW25113;
RX PubMed=19617347; DOI=10.1074/jbc.m109.036624;
RA Zhang Y., Yamaguchi Y., Inouye M.;
RT "Characterization of YafO, an Escherichia coli toxin.";
RL J. Biol. Chem. 284:25522-25531(2009).
RN [8]
RP FUNCTION AS AN MRNA INTERFERASE, INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=19943910; DOI=10.1111/j.1365-2958.2009.06969.x;
RA Christensen-Dalsgaard M., Jorgensen M.G., Gerdes K.;
RT "Three new RelE-homologous mRNA interferases of Escherichia coli
RT differentially induced by environmental stresses.";
RL Mol. Microbiol. 75:333-348(2010).
RN [9]
RP RETRACTED PAPER.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=21788497; DOI=10.1073/pnas.1100186108;
RA Maisonneuve E., Shakespeare L.J., Joergensen M.G., Gerdes K.;
RT "Bacterial persistence by RNA endonucleases.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:13206-13211(2011).
RN [10]
RP RETRACTION NOTICE OF PUBMED:21788497.
RX PubMed=29531044; DOI=10.1073/pnas.1803278115;
RA Maisonneuve E., Shakespeare L.J., Joergensen M.G., Gerdes K.;
RL Proc. Natl. Acad. Sci. U.S.A. 115:E2901-E2901(2018).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. A
CC translation-dependent mRNA interferase. Overexpression causes cessation
CC of cell growth and inhibits cell proliferation via inhibition of
CC translation; this blockage is overcome by subsequent expression of
CC antitoxin YafN. Overexpression causes cleavage of a number of mRNAs in
CC a ribosome-dependent fashion. YafO binding to the 50S ribosomal subunit
CC in the translation complex induces mRNA cleavage 3' to the region
CC protected by the ribosome; YafO alone is not able to digest mRNA.
CC {ECO:0000269|PubMed:19617347, ECO:0000269|PubMed:19943910}.
CC -!- SUBUNIT: Probably forms a complex with the antitoxin YafN which
CC inhibits the mRNA interferase activity. {ECO:0000305}.
CC -!- INTERACTION:
CC Q47157; Q47156: yafN; NbExp=2; IntAct=EBI-1119355, EBI-1114856;
CC -!- INDUCTION: Induced by amino acid starvation, glucose starvation, the
CC DNA cross-linker mitomycin C (SOS response) and when translation is
CC blocked. Induction is decreased in the absence of the Lon protease
CC suggesting, by homology to other toxin-antitoxin systems, that Lon may
CC degrade the YafN antitoxin. Transcription is negatively regulated by
CC the cognate locus, probably by YafN. A member of the dinB-yafNOP
CC operon; it has 2 promoters, 1 upstream of dinB and 1 specific for yafN-
CC yfaO. {ECO:0000269|PubMed:12813093, ECO:0000269|PubMed:19943910}.
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DR EMBL; D38582; BAA07595.1; -; Genomic_DNA.
DR EMBL; U70214; AAB08653.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73337.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA77902.1; -; Genomic_DNA.
DR PIR; B64748; B64748.
DR RefSeq; NP_414768.1; NC_000913.3.
DR RefSeq; WP_001263489.1; NZ_SSZK01000050.1.
DR AlphaFoldDB; Q47157; -.
DR BioGRID; 4259763; 133.
DR ComplexPortal; CPX-6026; YafNO toxin-antitoxin complex.
DR IntAct; Q47157; 7.
DR STRING; 511145.b0233; -.
DR PaxDb; Q47157; -.
DR PRIDE; Q47157; -.
DR DNASU; 944916; -.
DR EnsemblBacteria; AAC73337; AAC73337; b0233.
DR EnsemblBacteria; BAA77902; BAA77902; BAA77902.
DR GeneID; 944916; -.
DR KEGG; ecj:JW0223; -.
DR KEGG; eco:b0233; -.
DR PATRIC; fig|1411691.4.peg.2050; -.
DR EchoBASE; EB2946; -.
DR eggNOG; ENOG502ZEGB; Bacteria.
DR HOGENOM; CLU_148663_2_0_6; -.
DR OMA; IAIIKHW; -.
DR BioCyc; EcoCyc:G6117-MON; -.
DR BioCyc; MetaCyc:G6117-MON; -.
DR PRO; PR:Q47157; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0110001; C:toxin-antitoxin complex; IPI:ComplexPortal.
DR GO; GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
DR GO; GO:0043023; F:ribosomal large subunit binding; IDA:EcoCyc.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0017148; P:negative regulation of translation; IDA:EcoCyc.
DR GO; GO:0040008; P:regulation of growth; IC:ComplexPortal.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IC:ComplexPortal.
DR GO; GO:0009432; P:SOS response; IC:ComplexPortal.
DR InterPro; IPR020353; Toxin_YafO.
DR Pfam; PF13957; YafO_toxin; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome; RNA-binding; SOS response; Stress response;
KW Toxin-antitoxin system.
FT CHAIN 1..132
FT /note="mRNA interferase toxin YafO"
FT /id="PRO_0000168539"
SQ SEQUENCE 132 AA; 15488 MW; 6EAC0D9E38599CCF CRC64;
MRVFKTKLIR LQLTAEELDA LTADFISYKR DGVLPDIFGR DALYDDSFTW PLIKFERVAH
IHLANENNPF PPQLRQFSRT NDEAHLVYCQ GAFDEQAWLL IAILKPEPHK LARDNNQMHK
IGKMAEAFRM RF
