ID   YAFT_ECOLI              Reviewed;         261 AA.
AC   P77339;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Uncharacterized lipoprotein YafT;
DE   Flags: Precursor;
GN   Name=yafT; OrderedLocusNames=b0217, JW0206;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
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DR   EMBL; U70214; AAB08638.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73321.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77887.1; -; Genomic_DNA.
DR   PIR; B64746; B64746.
DR   RefSeq; NP_414752.1; NC_000913.3.
DR   RefSeq; WP_001086141.1; NZ_SSZK01000029.1.
DR   AlphaFoldDB; P77339; -.
DR   BioGRID; 4259758; 13.
DR   IntAct; P77339; 1.
DR   STRING; 511145.b0217; -.
DR   PaxDb; P77339; -.
DR   PRIDE; P77339; -.
DR   EnsemblBacteria; AAC73321; AAC73321; b0217.
DR   EnsemblBacteria; BAA77887; BAA77887; BAA77887.
DR   GeneID; 949009; -.
DR   KEGG; ecj:JW0206; -.
DR   KEGG; eco:b0217; -.
DR   PATRIC; fig|1411691.4.peg.2068; -.
DR   EchoBASE; EB3116; -.
DR   eggNOG; ENOG502ZBD8; Bacteria.
DR   HOGENOM; CLU_093098_0_0_6; -.
DR   OMA; MSKYYTV; -.
DR   BioCyc; EcoCyc:G6101-MON; -.
DR   PRO; PR:P77339; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           19..261
FT                   /note="Uncharacterized lipoprotein YafT"
FT                   /id="PRO_0000013785"
FT   LIPID           19
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           19
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   261 AA;  29606 MW;  3EA61CC9E636CF7F CRC64;
     MNSKKLCCIC VLFSLLAGCA SESSIDEKKK KAQVTQSNIN KNTPQQLTDK DLFGNETTLA
     VSEEDIQAAL DGDEFRVPLN SPVILVQSGN RAPETIMQEE MRKYYTVSTF SGIPDRQKPL
     TCNKNKDKNE NEDVASAENM NWMQALRFVA AKGHQKAIIV YQDMLQTGKY DSALKSTVWS
     DYKNDKLTDA ISLRYLVRFT LVDVATGEWA TWSPVNYEYK VLPPLPDKNE ASTTDMTEQQ
     IMQLKQKTYK AMVKDLVNRY Q