ID   YAFW_ECOLI              Reviewed;         105 AA.
AC   Q47684;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Antitoxin YafW {ECO:0000303|PubMed:14594833};
GN   Name=yafW; OrderedLocusNames=b0246, JW0235;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION AS AN ANTITOXIN.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=14594833; DOI=10.1128/jb.185.22.6600-6608.2003;
RA   Brown J.M., Shaw K.J.;
RT   "A novel family of Escherichia coli toxin-antitoxin gene pairs.";
RL   J. Bacteriol. 185:6600-6608(2003).
RN   [6]
RP   FUNCTION AS AN ANTITOXIN, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / BW25113;
RX   PubMed=28257056; DOI=10.3390/toxins9030077;
RA   Wen Z., Wang P., Sun C., Guo Y., Wang X.;
RT   "Interaction of type IV toxin/antitoxin systems in cryptic prophages of
RT   Escherichia coli K-12.";
RL   Toxins 9:0-0(2017).
CC   -!- FUNCTION: Antitoxin component of a type IV toxin-antitoxin (TA) system.
CC       Antitoxin that counteracts the effect of cognate toxin YkfI
CC       (PubMed:14594833, PubMed:28257056). It does not seem to bind to the
CC       cognate toxin but instead induces toxin loss by an unknown mechanism
CC       (PubMed:14594833). Co-overexpression of toxin YkfI and antitoxin YafW
CC       leads to formation of elongated cells (PubMed:28257056).
CC       {ECO:0000269|PubMed:14594833, ECO:0000269|PubMed:28257056}.
CC   -!- INDUCTION: Expressed in mid-log phase at considerably lower levels than
CC       antitoxin relB. {ECO:0000269|PubMed:28257056}.
CC   -!- DISRUPTION PHENOTYPE: Single deletion has no effect on expression of
CC       cognate toxin ykfI i.e. the probable operon is not autoregulatory
CC       (PubMed:28257056). Deletion of 3 type IV antitoxin genes (cbeA, yafW,
CC       yfjZ) has no effect on cell growth (PubMed:28257056).
CC       {ECO:0000269|PubMed:28257056}.
CC   -!- MISCELLANEOUS: Encoded in prophage CP4-6. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CbeA/YafW/YfjZ antitoxin family.
CC       {ECO:0000305}.
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DR   EMBL; U70214; AAB08665.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73349.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77914.1; -; Genomic_DNA.
DR   PIR; F64749; F64749.
DR   RefSeq; NP_414780.1; NC_000913.3.
DR   RefSeq; WP_000070395.1; NZ_LN832404.1.
DR   AlphaFoldDB; Q47684; -.
DR   SMR; Q47684; -.
DR   BioGRID; 4263051; 43.
DR   STRING; 511145.b0246; -.
DR   PaxDb; Q47684; -.
DR   PRIDE; Q47684; -.
DR   EnsemblBacteria; AAC73349; AAC73349; b0246.
DR   EnsemblBacteria; BAA77914; BAA77914; BAA77914.
DR   GeneID; 944929; -.
DR   KEGG; ecj:JW0235; -.
DR   KEGG; eco:b0246; -.
DR   PATRIC; fig|1411691.4.peg.2037; -.
DR   EchoBASE; EB3119; -.
DR   eggNOG; ENOG5032T1E; Bacteria.
DR   HOGENOM; CLU_144696_1_0_6; -.
DR   OMA; HAGFIYE; -.
DR   PhylomeDB; Q47684; -.
DR   BioCyc; EcoCyc:G6121-MON; -.
DR   PRO; PR:Q47684; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0051495; P:positive regulation of cytoskeleton organization; IEA:InterPro.
DR   InterPro; IPR009320; Antitoxin_CbeA.
DR   InterPro; IPR038025; CbeA_sf.
DR   Pfam; PF06154; CbeA_antitoxin; 1.
DR   SUPFAM; SSF143737; SSF143737; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..105
FT                   /note="Antitoxin YafW"
FT                   /id="PRO_0000168544"
SQ   SEQUENCE   105 AA;  11940 MW;  30DCF5DFDA2AA05E CRC64;
     MSNPTRGLQR EITLRLGARL VQEGNRLHYL ADRASITGKF SDIECRKLDE TFPHFILQME
     SMLTTGELSP HHAHCVTLYH NDLTCEADTL GSCGYVYIAI YPTQR