YAFY_ECOLI
ID YAFY_ECOLI Reviewed; 147 AA.
AC P77365;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Lipoprotein YafY;
DE Flags: Precursor;
GN Name=yafY; OrderedLocusNames=b0251, JW0240;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT - 6.0 min (189,987 - 281,416bp) region.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION IN DEGP EXPRESSION, SUBCELLULAR LOCATION, PALMITOYLATION AT
RP CYS-21, AND MUTAGENESIS OF CYS-21; 22-ASP-ASP-23; ASP-60; TYR-61 AND
RP GLN-136.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=15252048; DOI=10.1074/jbc.m406390200;
RA Miyadai H., Tanaka-Masuda K., Matsuyama S., Tokuda H.;
RT "Effects of lipoprotein overproduction on the induction of DegP (HtrA)
RT involved in quality control in the Escherichia coli periplasm.";
RL J. Biol. Chem. 279:39807-39813(2004).
CC -!- FUNCTION: When overproduced strongly induces degP through the
CC activation of the two-component envelope stress response system
CC CpxA/CpxR (PubMed:15252048). {ECO:0000269|PubMed:15252048}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:15252048}; Lipid-anchor
CC {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000305|PubMed:15252048}.
CC -!- SIMILARITY: To E.coli YfjS. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB08670.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA77920.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U70214; AAB08670.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73354.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA77920.1; ALT_INIT; Genomic_DNA.
DR PIR; C64750; C64750.
DR RefSeq; NP_414785.4; NC_000913.3.
DR RefSeq; WP_000824223.1; NZ_LN832404.1.
DR AlphaFoldDB; P77365; -.
DR IntAct; P77365; 3.
DR STRING; 511145.b0251; -.
DR PaxDb; P77365; -.
DR PRIDE; P77365; -.
DR EnsemblBacteria; AAC73354; AAC73354; b0251.
DR EnsemblBacteria; BAA77920; BAA77920; BAA77920.
DR GeneID; 63141108; -.
DR GeneID; 944934; -.
DR KEGG; ecj:JW0240; -.
DR KEGG; eco:b0251; -.
DR PATRIC; fig|511145.12.peg.253; -.
DR EchoBASE; EB3121; -.
DR eggNOG; COG2378; Bacteria.
DR eggNOG; ENOG5032TZC; Bacteria.
DR HOGENOM; CLU_975685_0_0_6; -.
DR BioCyc; EcoCyc:G6126-MON; -.
DR PRO; PR:P77365; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 21..147
FT /note="Lipoprotein YafY"
FT /id="PRO_0000050270"
FT LIPID 21
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT ECO:0000305|PubMed:15252048"
FT LIPID 21
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT MUTAGEN 21
FT /note="C->A: Very poorly expressed."
FT /evidence="ECO:0000269|PubMed:15252048"
FT MUTAGEN 22..23
FT /note="DD->SS: Still induces degP when overexpressed,
FT remains in inner membrane."
FT /evidence="ECO:0000269|PubMed:15252048"
FT MUTAGEN 60
FT /note="D->G: 5-fold decrease in degP induction when
FT overexpressed."
FT /evidence="ECO:0000269|PubMed:15252048"
FT MUTAGEN 61
FT /note="Y->F: 3-fold decrease in degP induction when
FT overexpressed."
FT /evidence="ECO:0000269|PubMed:15252048"
FT MUTAGEN 136
FT /note="Q->R: 2-fold decrease in degP induction when
FT overexpressed."
FT /evidence="ECO:0000269|PubMed:15252048"
SQ SEQUENCE 147 AA; 16815 MW; DB68B4C16A7ECD94 CRC64;
MKRKTLPLLA LVATTLFLIA CDDRSDDLKA ISKFKDLTPP RFSDVVSHQD DVSEEWSQVD
YLSGPTLQVL RTRQSPDGCE DGSYYYLVDM QEKTVQPLMN ALCIADNIKL EYQEVTDPYT
KEKYFEYAHD GKLMGQLLIP SNPDNQE
