ID   YAG5_STAAU              Reviewed;         261 AA.
AC   P55177;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Hydrolase in agr operon;
DE            EC=3.5.-.-;
DE   AltName: Full=ORF 5;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Isolate GAL;
RX   PubMed=7565609; DOI=10.1007/bf02191645;
RA   Novick R.P., Projan S.J., Kornblum J., Ross H.F., Ji G., Kreiswirth B.,
RA   Vandenesch F., Moghazeh S.;
RT   "The agr P2 operon: an autocatalytic sensory transduction system in
RT   Staphylococcus aureus.";
RL   Mol. Gen. Genet. 248:446-458(1995).
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       NIT1/NIT2 family. {ECO:0000305}.
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DR   EMBL; X52543; CAA36779.1; -; Genomic_DNA.
DR   PIR; S20793; S20793.
DR   RefSeq; WP_000867960.1; NZ_WOFG01000001.1.
DR   AlphaFoldDB; P55177; -.
DR   SMR; P55177; -.
DR   OMA; KIHRFGF; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR001110; UPF0012_CS.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS01227; UPF0012; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..261
FT                   /note="Hydrolase in agr operon"
FT                   /id="PRO_0000213264"
FT   DOMAIN          1..239
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        41
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        110
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        146
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ   SEQUENCE   261 AA;  29826 MW;  2303D95A8C18F838 CRC64;
     MKVQIYQLPI VFGDSSKNET QITQWFEKNM NAEVDVVVLP EMWNNGYDLE HLNEKADNNL
     GQSFSFIKHL AEKYKVDIVA GSVSNIRNNQ IFNTAFSVNK SGQLINEYDK VHLVPMLREH
     EFLTAGEYVA EPFQLSDGTY VTQLICYDLR FPELLRYPAR SGAKIAFYVA QWPMSRLQHW
     HSLLKARAIE NNMFVIGTNS TGFDGNTEYA GHSIVINPNG DLVGELNESA DILTVDLNLN
     EVEQQRENIP VFKSIKLDLY K