YAJ8_YEAST
ID YAJ8_YEAST Reviewed; 234 AA.
AC P39548; D6VPN0;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=DUP240 protein YAR028W;
GN OrderedLocusNames=YAR028W; ORFNames=FUN56;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RA Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B.,
RA Kaback D.B., Clark M.W.;
RT "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52
RT Kbp CDC15-FLO1-PHO11-YAR074 region.";
RL Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA Storms R.K.;
RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=12101299; DOI=10.1099/00221287-148-7-2111;
RA Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L.,
RA Jauniaux J.-C.;
RT "Functional analysis of the Saccharomyces cerevisiae DUP240 multigene
RT family reveals membrane-associated proteins that are not essential for cell
RT viability.";
RL Microbiology 148:2111-2123(2002).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-217, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=14557538; DOI=10.1073/pnas.2135500100;
RA Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.;
RT "A subset of membrane-associated proteins is ubiquitinated in response to
RT mutations in the endoplasmic reticulum degradation machinery.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [8]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-217, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Members of the DUP240 multigene family are
CC specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240
CC proteins show no obvious alterations in mating, sporulation and cell
CC growth. {ECO:0000269|PubMed:12101299}.
CC -!- MISCELLANEOUS: Present with 1835 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DUP/COS family. {ECO:0000305}.
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DR EMBL; L28920; AAC09490.1; -; Genomic_DNA.
DR EMBL; BK006935; DAA07000.1; -; Genomic_DNA.
DR PIR; S53480; S53480.
DR RefSeq; NP_009415.1; NM_001178222.1.
DR AlphaFoldDB; P39548; -.
DR BioGRID; 31807; 75.
DR DIP; DIP-7826N; -.
DR IntAct; P39548; 7.
DR MINT; P39548; -.
DR STRING; 4932.YAR028W; -.
DR iPTMnet; P39548; -.
DR MaxQB; P39548; -.
DR PaxDb; P39548; -.
DR PRIDE; P39548; -.
DR EnsemblFungi; YAR028W_mRNA; YAR028W; YAR028W.
DR GeneID; 851280; -.
DR KEGG; sce:YAR028W; -.
DR SGD; S000000076; YAR028W.
DR VEuPathDB; FungiDB:YAR028W; -.
DR eggNOG; ENOG502SSNW; Eukaryota.
DR GeneTree; ENSGT00940000176285; -.
DR HOGENOM; CLU_081384_0_1_1; -.
DR InParanoid; P39548; -.
DR OMA; CHEFFTT; -.
DR BioCyc; YEAST:G3O-28879-MON; -.
DR PRO; PR:P39548; -.
DR Proteomes; UP000002311; Chromosome I.
DR RNAct; P39548; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR001142; DUP/COS.
DR Pfam; PF00674; DUP; 1.
PE 1: Evidence at protein level;
KW Isopeptide bond; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Ubl conjugation.
FT CHAIN 1..234
FT /note="DUP240 protein YAR028W"
FT /id="PRO_0000207529"
FT TOPO_DOM 1..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..76
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..234
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 168..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 217
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 234 AA; 26458 MW; 30EE6C11593FA8F3 CRC64;
MQTPSENTDV KMDTLDEPSA HLIEENVALP EDTFSSHLSY VLYEIAHCKP IMFMIIIIVS
LISLIVLFHD NDGCTVILVM SLIVASMALM VVAAFTFGKA ITEQEFMIKL LVEVIARKPA
GKEWGTVAYN MNQYLFMKRL WYTPYYFYSG KKCHEFFTTL IKEVNSGSHS DSSSNSAEDT
QSPVSAGKTS NGLNNFYSIR SDPILMAYVL KATQIEKEAQ SEYWRKQYPD ADLP
