YAJC_ECOLI
ID YAJC_ECOLI Reviewed; 110 AA.
AC P0ADZ7; P19677; Q2MC19;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Sec translocon accessory complex subunit YajC;
GN Name=yajC; OrderedLocusNames=b0407, JW0397;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2170107; DOI=10.1002/j.1460-2075.1990.tb07519.x;
RA Gardel C., Johnson K., Jacq A., Beckwith J.;
RT "The secD locus of E.coli codes for two membrane proteins required for
RT protein export.";
RL EMBO J. 9:3209-3216(1990).
RN [2]
RP ERRATUM OF PUBMED:2170107.
RX PubMed=2249673; DOI=10.1002/j.1460-2075.1990.tb07645.x;
RA Gardel C., Johnson K., Jacq A., Beckwith J.;
RL EMBO J. 9:4205-4206(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1706703; DOI=10.1128/jb.173.7.2256-2264.1991;
RA Reuter K., Slany R., Ullrich F., Kersten H.;
RT "Structure and organization of Escherichia coli genes involved in
RT biosynthesis of the deazaguanine derivative queuine, a nutrient factor for
RT eukaryotes.";
RL J. Bacteriol. 173:2256-2264(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7507921; DOI=10.1128/jb.176.3.804-814.1994;
RA Pogliano K.J., Beckwith J.;
RT "Genetic and molecular characterization of the Escherichia coli secD operon
RT and its products.";
RL J. Bacteriol. 176:804-814(1994).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [8]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=BL21-DE3;
RX PubMed=16079137; DOI=10.1074/jbc.m506479200;
RA Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L.,
RA von Heijne G., Daley D.O.;
RT "Protein complexes of the Escherichia coli cell envelope.";
RL J. Biol. Chem. 280:34409-34419(2005).
RN [9]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=BL21-DE3;
RX PubMed=27435098; DOI=10.1042/bcj20160545;
RA Komar J., Alvira S., Schulze R.J., Martin R., Lycklama a Nijeholt J.A.,
RA Lee S.C., Dafforn T.R., Deckers-Hebestreit G., Berger I., Schaffitzel C.,
RA Collinson I.;
RT "Membrane protein insertion and assembly by the bacterial holo-translocon
RT SecYEG-SecDF-YajC-YidC.";
RL Biochem. J. 473:3341-3354(2016).
CC -!- FUNCTION: The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein
CC secretase/insertase is a supercomplex required for protein secretion,
CC insertion of proteins into membranes, and assembly of membrane protein
CC complexes (PubMed:27435098). The SecYEG complex is essential for
CC assembly of a number of proteins and complexes, assembly is facilitated
CC in the presence of the SecDF-YajC-YidC subcomplex (PubMed:27435098).
CC {ECO:0000269|PubMed:27435098}.
CC -!- SUBUNIT: Part of the SecDF-YidC-YajC translocase complex; YajC is also
CC present in a homooligomeric complex in excess of the other components
CC (PubMed:16079137). The SecDF-YidC-YajC translocase forms a supercomplex
CC with SecYEG, called the holo-translocon (HTL) (PubMed:27435098). The
CC stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC
CC is in the reconstituted complex (with SecDF) but as no antibody is
CC available it could not be quantified (PubMed:27435098).
CC {ECO:0000269|PubMed:16079137, ECO:0000269|PubMed:27435098}.
CC -!- INTERACTION:
CC P0ADZ7; P31224: acrB; NbExp=2; IntAct=EBI-1130723, EBI-551006;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:16079137,
CC ECO:0000269|PubMed:27435098}; Single-pass membrane protein
CC {ECO:0000269|PubMed:16079137}.
CC -!- SIMILARITY: Belongs to the YajC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X56175; CAA39633.1; -; Genomic_DNA.
DR EMBL; M63939; AAA24668.1; -; Genomic_DNA.
DR EMBL; S68715; AAC60468.1; -; Genomic_DNA.
DR EMBL; U82664; AAB40163.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73510.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76187.1; -; Genomic_DNA.
DR PIR; D38530; D38530.
DR RefSeq; NP_414941.1; NC_000913.3.
DR RefSeq; WP_000007629.1; NZ_STEB01000007.1.
DR PDB; 2RDD; X-ray; 3.50 A; B=19-55.
DR PDBsum; 2RDD; -.
DR AlphaFoldDB; P0ADZ7; -.
DR SMR; P0ADZ7; -.
DR BioGRID; 4263532; 415.
DR ComplexPortal; CPX-1095; Holo-translocon SecYEG-SecDF-YajC-YidC complex.
DR DIP; DIP-29531N; -.
DR IntAct; P0ADZ7; 2.
DR STRING; 511145.b0407; -.
DR TCDB; 3.A.5.1.1; the general secretory pathway (sec) family.
DR jPOST; P0ADZ7; -.
DR PaxDb; P0ADZ7; -.
DR PRIDE; P0ADZ7; -.
DR EnsemblBacteria; AAC73510; AAC73510; b0407.
DR EnsemblBacteria; BAE76187; BAE76187; BAE76187.
DR GeneID; 67416520; -.
DR GeneID; 945374; -.
DR KEGG; ecj:JW0397; -.
DR KEGG; eco:b0407; -.
DR PATRIC; fig|1411691.4.peg.1870; -.
DR EchoBASE; EB1088; -.
DR eggNOG; COG1862; Bacteria.
DR HOGENOM; CLU_116157_2_1_6; -.
DR InParanoid; P0ADZ7; -.
DR OMA; GMEMIIM; -.
DR PhylomeDB; P0ADZ7; -.
DR BioCyc; EcoCyc:EG11096-MON; -.
DR BioCyc; MetaCyc:EG11096-MON; -.
DR EvolutionaryTrace; P0ADZ7; -.
DR PRO; PR:P0ADZ7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0031522; C:cell envelope Sec protein transport complex; IPI:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0032978; P:protein insertion into membrane from inner side; IDA:ComplexPortal.
DR GO; GO:0043952; P:protein transport by the Sec complex; IDA:ComplexPortal.
DR InterPro; IPR003849; Preprotein_translocase_YajC.
DR PANTHER; PTHR33909; PTHR33909; 1.
DR Pfam; PF02699; YajC; 1.
DR PRINTS; PR01853; YAJCTRNLCASE.
DR SMART; SM01323; YajC; 1.
DR TIGRFAMs; TIGR00739; yajC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..110
FT /note="Sec translocon accessory complex subunit YajC"
FT /id="PRO_0000097013"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 82
FT /note="A -> R (in Ref. 1; CAA39633)"
FT /evidence="ECO:0000305"
FT HELIX 22..37
FT /evidence="ECO:0007829|PDB:2RDD"
FT HELIX 39..49
FT /evidence="ECO:0007829|PDB:2RDD"
FT HELIX 52..54
FT /evidence="ECO:0007829|PDB:2RDD"
SQ SEQUENCE 110 AA; 11887 MW; 8E90AF51F0205306 CRC64;
MSFFISDAVA ATGAPAQGSP MSLILMLVVF GLIFYFMILR PQQKRTKEHK KLMDSIAKGD
EVLTNGGLVG RVTKVAENGY IAIALNDTTE VVIKRDFVAA VLPKGTMKAL
