YAJI_ECOLI
ID YAJI_ECOLI Reviewed; 179 AA.
AC P46122; Q2MC14;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 3.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Uncharacterized lipoprotein YajI;
DE Flags: Precursor;
GN Name=yajI; OrderedLocusNames=b0412, JW5056;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Lin D., Allen E., Araujo R., Aparicio A.M., Botstein D., Cherry M.,
RA Chung E., Dietrich F., Duncan M., Federspiel N., Kalman S., Kim K.,
RA Komp C., Lashkari D., Lew H., Namath A., Oefner P., Schroff N., Winant A.,
RA Davis R.;
RL Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
RC STRAIN=K12;
RX PubMed=1406588; DOI=10.1007/bf00538702;
RA Taura T., Ueguchi C., Shiba K., Ito K.;
RT "Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive
RT growth of Escherichia coli and suppression of the secY24 mutation.";
RL Mol. Gen. Genet. 234:429-432(1992).
RN [6]
RP IDENTIFICATION.
RX PubMed=7567469; DOI=10.1093/nar/23.17.3554;
RA Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.;
RT "Detection of new genes in a bacterial genome using Markov models for three
RT gene classes.";
RL Nucleic Acids Res. 23:3554-3562(1995).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA74983.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAB40168.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U31810; AAA74983.1; ALT_INIT; Genomic_DNA.
DR EMBL; U82664; AAB40168.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73515.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76192.1; -; Genomic_DNA.
DR EMBL; X64395; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; D64770; D64770.
DR RefSeq; NP_414946.4; NC_000913.3.
DR RefSeq; WP_001326929.1; NZ_SSZK01000009.1.
DR PDB; 2JWY; NMR; -; A=21-179.
DR PDBsum; 2JWY; -.
DR AlphaFoldDB; P46122; -.
DR BMRB; P46122; -.
DR SMR; P46122; -.
DR BioGRID; 4261730; 286.
DR STRING; 511145.b0412; -.
DR jPOST; P46122; -.
DR PaxDb; P46122; -.
DR PRIDE; P46122; -.
DR EnsemblBacteria; AAC73515; AAC73515; b0412.
DR EnsemblBacteria; BAE76192; BAE76192; BAE76192.
DR GeneID; 947233; -.
DR KEGG; ecj:JW5056; -.
DR KEGG; eco:b0412; -.
DR PATRIC; fig|1411691.4.peg.1865; -.
DR EchoBASE; EB2712; -.
DR eggNOG; COG4238; Bacteria.
DR HOGENOM; CLU_100508_0_0_6; -.
DR OMA; GTVEWGQ; -.
DR PhylomeDB; P46122; -.
DR BioCyc; EcoCyc:EG12874-MON; -.
DR EvolutionaryTrace; P46122; -.
DR PRO; PR:P46122; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.1620; -; 1.
DR InterPro; IPR021658; DUF3251.
DR InterPro; IPR037125; YajI-like_sf.
DR Pfam; PF11622; DUF3251; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..179
FT /note="Uncharacterized lipoprotein YajI"
FT /id="PRO_0000018044"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT HELIX 45..59
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 61..66
FT /evidence="ECO:0007829|PDB:2JWY"
FT TURN 68..70
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 82..92
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 94..109
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 114..123
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 132..142
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 147..149
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 151..158
FT /evidence="ECO:0007829|PDB:2JWY"
FT HELIX 163..165
FT /evidence="ECO:0007829|PDB:2JWY"
FT STRAND 168..176
FT /evidence="ECO:0007829|PDB:2JWY"
SQ SEQUENCE 179 AA; 19560 MW; FF52BE4B657818CC CRC64;
MNTNVFRLLL LGSLFSLSAC VQQSEVRQMK HSVSTLNQEM TQLNQETVKI TQQNRLNAKS
SSGVYLLPGA KTPARLESQI GTLRMSLVNI TPDADGTTLT LRIQGESNDP LPAFSGTVEY
GQIQGTIDNF QEINVQNQLI NAPASVLAPS DVDIPLQLKG ISVDQLGFVR IHDIQPVMQ
