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YANC_ASPNA
ID   YANC_ASPNA              Reviewed;         537 AA.
AC   G3Y416;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Cytochrome P450 monooxygenase yanC {ECO:0000303|PubMed:24684908};
DE            EC=1.-.-.- {ECO:0000305|PubMed:24684908};
DE   AltName: Full=Yanuthone D biosynthesis cluster protein C {ECO:0000303|PubMed:24684908};
DE   Flags: Precursor;
GN   Name=yanC {ECO:0000303|PubMed:24684908}; ORFNames=ASPNIDRAFT_54844;
OS   Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 /
OS   NCTC 3858a / NRRL 328 / USDA 3528.7).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=380704;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL
RC   328 / USDA 3528.7;
RX   PubMed=21543515; DOI=10.1101/gr.112169.110;
RA   Andersen M.R., Salazar M.P., Schaap P.J., van de Vondervoort P.J.I.,
RA   Culley D., Thykaer J., Frisvad J.C., Nielsen K.F., Albang R., Albermann K.,
RA   Berka R.M., Braus G.H., Braus-Stromeyer S.A., Corrochano L.M., Dai Z.,
RA   van Dijck P.W.M., Hofmann G., Lasure L.L., Magnuson J.K., Menke H.,
RA   Meijer M., Meijer S.L., Nielsen J.B., Nielsen M.L., van Ooyen A.J.J.,
RA   Pel H.J., Poulsen L., Samson R.A., Stam H., Tsang A., van den Brink J.M.,
RA   Atkins A., Aerts A., Shapiro H., Pangilinan J., Salamov A., Lou Y.,
RA   Lindquist E., Lucas S., Grimwood J., Grigoriev I.V., Kubicek C.P.,
RA   Martinez D., van Peij N.N.M.E., Roubos J.A., Nielsen J., Baker S.E.;
RT   "Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015
RT   versus enzyme-producing CBS 513.88.";
RL   Genome Res. 21:885-897(2011).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=11031048; DOI=10.1021/jo0006831;
RA   Bugni T.S., Abbanat D., Bernan V.S., Maiese W.M., Greenstein M.,
RA   Van Wagoner R.M., Ireland C.M.;
RT   "Yanuthones: novel metabolites from a marine isolate of Aspergillus
RT   niger.";
RL   J. Org. Chem. 65:7195-7200(2000).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24684908; DOI=10.1016/j.chembiol.2014.01.013;
RA   Holm D.K., Petersen L.M., Klitgaard A., Knudsen P.B., Jarczynska Z.D.,
RA   Nielsen K.F., Gotfredsen C.H., Larsen T.O., Mortensen U.H.;
RT   "Molecular and chemical characterization of the biosynthesis of the 6-MSA-
RT   derived meroterpenoid yanuthone D in Aspergillus niger.";
RL   Chem. Biol. 21:519-529(2014).
CC   -!- FUNCTION: Cytochrome P450 monooxygenase; part of the gene cluster that
CC       mediates the biosynthesis of yanuthone D, a fungal isoprenoid
CC       epoxycyclohexenone that acts as an antibiotic against fungi and
CC       bacteria (PubMed:24684908). The first step of the pathway is the
CC       synthesis of 6-methylsalicylic acid (6-MSA) by the polyketide synthase
CC       yanA (PubMed:24684908). 6-MSA is then converted to m-cresol by the
CC       decarboxylase yanB (PubMed:24684908). The cytochrome P450 monooxygenase
CC       yanC then catalyzes the oxidation of m-cresol to toluquinol
CC       (PubMed:24684908). Epoxidation of toluquinol is then performed by the
CC       short chain dehydrogenase yanD, with the help of yanE, and a further
CC       prenylation by yanG leads to 7-deacetoxyyanuthone A (PubMed:24684908).
CC       The next step is the hydroxylation of C-22 of 7-deacetoxyyanuthone A by
CC       the cytochrome P450 monooxygenase yanH to yield 22-deacetylyanuthone A
CC       (PubMed:24684908). O-Mevalon transferase yanI then attaches mevalon to
CC       the hydroxyl group of 22-deacetylyanuthone A to produce yanuthone E
CC       (PubMed:24684908). Finally, the FAD-dependent monooxygenase yanF
CC       oxidizes the hydroxyl group at C15 of yanuthone E to form yanuthone D
CC       (PubMed:24684908). Furthermore, several branching points in the pathway
CC       lead to the production of yanuthones F and G from 7-deacetoxyyanuthone
CC       A; yanuthones H and I from 22-deacetylyanuthone A; and yanuthone J from
CC       yanuthone E (PubMed:24684908). YanC is also involved in the synthesis
CC       of yanuthone X1 which does not have 6-methylsalicylic acid (6-MSA) as
CC       precursor (PubMed:24684908). {ECO:0000269|PubMed:24684908}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P04798};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:24684908}.
CC   -!- DISRUPTION PHENOTYPE: Loses the ability to produce yanuthone D
CC       (PubMed:24684908). Leads also to the loss of production of yanuthone X1
CC       which does not have 6-methylsalicylic acid (6-MSA) as a precursor
CC       (PubMed:24684908). {ECO:0000269|PubMed:24684908}.
CC   -!- BIOTECHNOLOGY: Yanuthone D is an antibiotic against C.albicans,
CC       methicillin-resistant S.aureus (MRSA), and vancomycin-resistant
CC       Enterococcus (PubMed:11031048). {ECO:0000269|PubMed:11031048}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; ACJE01000012; EHA22193.1; -; Genomic_DNA.
DR   AlphaFoldDB; G3Y416; -.
DR   SMR; G3Y416; -.
DR   STRING; 380704.G3Y416; -.
DR   EnsemblFungi; EHA22193; EHA22193; ASPNIDRAFT_54844.
DR   VEuPathDB; FungiDB:ASPNIDRAFT2_1107512; -.
DR   HOGENOM; CLU_001570_2_1_1; -.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000009038; Unassembled WGS sequence.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   1: Evidence at protein level;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..537
FT                   /note="Cytochrome P450 monooxygenase yanC"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000436762"
FT   BINDING         449
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
SQ   SEQUENCE   537 AA;  61653 MW;  72A28EFC65C67EC2 CRC64;
     MALVHLTALA ACGLLLVILR AAFNSWRLQR KLPPGPPGAP LIGNILQLPK VRAHQKFTEW
     ARTYGGLYSF RIGPATAAVV TDRALVKELF DKRSALYSSR PTSYVGQNII TRGDHLLVMD
     YSDNWRLFRK AINQHFMASM CEKTHVRLLE AEHTQMMRDF LLHPEKHMLH TKRTTNSIIM
     SLLFGIRTPS WDTPHMQELY EIMEIWSQIM ETGATPPVDI FPWLHWVPQQ WLGHWVDRSQ
     TVARGMKRLY SSFHRRAIEA RRKAESTSQS RARTFLDDVL DLQEKLGLTD NQVDFLGGVM
     MEGGSDTGST MLLVMIQALA LHPEIQQRAR AELDAVCGEH RSPTWEDFPR LPYINMIVKE
     TMRWRPVTPL AFPHALNKDD WVNGYFLPKG TTVFLNVWGL HHDENIFPNP DQFDPSRFEG
     RHKLAFDYAA SPDYMQRDHY IYGAGRRLCP GIHLSERSMF LGAAKLLWAF NFEPARDEDG
     NPIRIDTDPV TGYTEGFLVC PRPYQCNVTP RSPAHAETIL REFSRAESEV LSQYAMP
 
 
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