YAR1_YEAST
ID YAR1_YEAST Reviewed; 200 AA.
AC P46683; D6W3D2;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Ankyrin repeat-containing protein YAR1;
GN Name=YAR1; OrderedLocusNames=YPL239W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96902 / S288c / TD28;
RX PubMed=8675027; DOI=10.1016/0378-1119(96)00023-6;
RA Lycan D.E., Stafford K.A., Bollinger W., Breeden L.L.;
RT "A new Saccharomyces cerevisiae ankyrin repeat-encoding gene required for a
RT normal rate of cell proliferation.";
RL Gene 171:33-40(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 84-200.
RX PubMed=3136928; DOI=10.1016/s0092-8674(88)80006-0;
RA Donahue T.F., Cigan A.M., Pabich E.K., Valavicius B.C.;
RT "Mutations at a Zn(II) finger motif in the yeast eIF-2 beta gene alter
RT ribosomal start-site selection during the scanning process.";
RL Cell 54:621-632(1988).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for normal rate of cell proliferation.
CC -!- INTERACTION:
CC P46683; P05750: RPS3; NbExp=2; IntAct=EBI-20829, EBI-16140;
CC -!- MISCELLANEOUS: Present with 13100 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
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DR EMBL; U34385; AAB60315.1; -; Genomic_DNA.
DR EMBL; Z67751; CAA91605.1; -; Genomic_DNA.
DR EMBL; Z73595; CAA97960.1; -; Genomic_DNA.
DR EMBL; AY558138; AAS56464.1; -; Genomic_DNA.
DR EMBL; M21813; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006949; DAA11198.1; -; Genomic_DNA.
DR PIR; S61025; S61025.
DR RefSeq; NP_015085.1; NM_001184053.1.
DR PDB; 4BSZ; X-ray; 2.84 A; B=1-200.
DR PDBsum; 4BSZ; -.
DR AlphaFoldDB; P46683; -.
DR SMR; P46683; -.
DR BioGRID; 35924; 252.
DR IntAct; P46683; 4.
DR MINT; P46683; -.
DR STRING; 4932.YPL239W; -.
DR iPTMnet; P46683; -.
DR MaxQB; P46683; -.
DR PaxDb; P46683; -.
DR PRIDE; P46683; -.
DR EnsemblFungi; YPL239W_mRNA; YPL239W; YPL239W.
DR GeneID; 855837; -.
DR KEGG; sce:YPL239W; -.
DR SGD; S000006160; YAR1.
DR VEuPathDB; FungiDB:YPL239W; -.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_000134_20_0_1; -.
DR InParanoid; P46683; -.
DR OMA; HMVCANG; -.
DR BioCyc; YEAST:G3O-34125-MON; -.
DR PRO; PR:P46683; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P46683; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0030907; C:MBF transcription complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0033309; C:SBF transcription complex; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; IDA:SGD.
DR GO; GO:0034599; P:cellular response to oxidative stress; IMP:SGD.
DR GO; GO:0071931; P:positive regulation of transcription involved in G1/S transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0032880; P:regulation of protein localization; IMP:SGD.
DR GO; GO:0006970; P:response to osmotic stress; IMP:SGD.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:SGD.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IMP:SGD.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 1.
DR SMART; SM00248; ANK; 2.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
PE 1: Evidence at protein level;
KW 3D-structure; ANK repeat; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..200
FT /note="Ankyrin repeat-containing protein YAR1"
FT /id="PRO_0000067079"
FT REPEAT 49..78
FT /note="ANK 1"
FT REPEAT 92..121
FT /note="ANK 2"
FT REGION 152..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 78
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CONFLICT 118
FT /note="A -> P (in Ref. 5; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT HELIX 10..22
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 25..34
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 38..43
FT /evidence="ECO:0007829|PDB:4BSZ"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 53..59
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 63..76
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 79..86
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 96..102
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 106..114
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 130..136
FT /evidence="ECO:0007829|PDB:4BSZ"
FT HELIX 140..149
FT /evidence="ECO:0007829|PDB:4BSZ"
SQ SEQUENCE 200 AA; 22356 MW; 8089CFC062E65395 CRC64;
MGLHSEPLDQ EDQDTIILDA RAGDLDSLKD IFTTLVSPEL LSTCKESESD STALHMAAAN
GHIETVRYIL ETVSRANSAE DLKAFVNEVN KTGNTALHWA SLNGKLDVVK LLCDEYEADP
FIRNKFGHDA IFEAENSGKE EVETYFLKKY DVEPEDDEED TQTEGKNSVQ ITKGTEIEQV
TKEATEALRE ETEKLNINKD
