YB75_YEAST
ID YB75_YEAST Reviewed; 900 AA.
AC P38321; D6VQM1;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Uncharacterized protein YBR225W;
GN OrderedLocusNames=YBR225W; ORFNames=YBR1522;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- MISCELLANEOUS: Present with 623 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z36092; CAA85188.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07341.1; -; Genomic_DNA.
DR PIR; S46101; S46101.
DR RefSeq; NP_009784.1; NM_001178573.1.
DR AlphaFoldDB; P38321; -.
DR BioGRID; 32921; 95.
DR DIP; DIP-6382N; -.
DR IntAct; P38321; 15.
DR MINT; P38321; -.
DR STRING; 4932.YBR225W; -.
DR iPTMnet; P38321; -.
DR MaxQB; P38321; -.
DR PaxDb; P38321; -.
DR PRIDE; P38321; -.
DR EnsemblFungi; YBR225W_mRNA; YBR225W; YBR225W.
DR GeneID; 852526; -.
DR KEGG; sce:YBR225W; -.
DR SGD; S000000429; YBR225W.
DR VEuPathDB; FungiDB:YBR225W; -.
DR eggNOG; ENOG502QRP4; Eukaryota.
DR HOGENOM; CLU_011913_0_0_1; -.
DR InParanoid; P38321; -.
DR OMA; HIHPTKH; -.
DR BioCyc; YEAST:G3O-29159-MON; -.
DR PRO; PR:P38321; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38321; protein.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..900
FT /note="Uncharacterized protein YBR225W"
FT /id="PRO_0000202515"
FT REGION 1..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 103..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 512..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 568..613
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 648..676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..82
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 103..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..140
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..536
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 568..601
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 105
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 900 AA; 101236 MW; DDCD01F45CCA326A CRC64;
MGSNKEAKNI DSKNDRGLTS ITSNKISNLK AHDNHTSSMI TEHKNADKEK GKQEKESRNG
TTQSSSSVES HSPQVSHHSD KLSSFDSPLH LPNFRLADDL FSNSSRRSSD SAASSSVSKL
KSAQLSKIGL HHHHTSNNKH SHRSGTPTSE VKANYSPDPS APRFIVSNMV GNGRGGGGLH
GATSNVVKKL HSRKKWDWNT LPASDSSLLI KTVSGNHNLI NICIDGEFKQ IMYDPNHNEL
FNRMDLFLSF NMDSSPEDSL IFAKKRLRSY IDFLTKYLES RKYAFECYPF NIENIINIET
EVKCFPSFDP LKDYSEIESL IQLWLAQSQK FLLQSNSFFF SSEVVEELIK RKPTTRQHSN
PTISTTSNKI SDPTLYIQQL DIEANSPRPV ISDPLDEIDI LLIRPLHKTL GGWQLAYDEP
SLNIADFALD LSPWMIDSSD NDAQNKNASE IAPEYLTNLQ NYLPRKGSRA KIVSDEQEVI
ELNSSNASEY MYDCMNRKFF TDDAKERISR NNFNQGVEED PLNDQFASSR SLSLPSSGAD
AVKRKKSPTK ATKKSGFVNF FKRKHSQLAS TSHTTSPSVS PSISSSSSPK IQPQSHISSP
PRTEKAPHVK SANQAHQNEW LENFFCRTLN NYKEIDLPTQ FILPKEVKRS SNAQLQPEDE
PPLSSPISSN SDNSFPNEGL DRAKSAAIYG KEYLKLRLPF ASDTIPAVIC PWVWTSLSYY
KWKALLREIY RSIIPGGYAL AIVPDLRISN TYYTGILGNA DAEKANNSSE EFLTTKERDK
TFDAMAIDAI NKGLHIHPTK HLTRTFKDVG FTGIKSSVLS LKTGDFKTDM GFLNEFNSLD
MWDYMLRRQL PDSSCPPKDT DPTTLFKRYV EEHIGKIDDN AGCFRTLYVV AQKPKLPYTK
