CATIN_ARATH
ID CATIN_ARATH Reviewed; 716 AA.
AC F4I2J8; Q9ZWB1;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Cactin;
GN Name=CTN; OrderedLocusNames=At1g03910; ORFNames=F21M11.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND INTERACTION WITH
RP AT5G63440.
RX PubMed=23454656; DOI=10.1016/j.febslet.2013.02.041;
RA Baldwin K.L., Dinh E.M., Hart B.M., Masson P.H.;
RT "CACTIN is an essential nuclear protein in Arabidopsis and may be
RT associated with the eukaryotic spliceosome.";
RL FEBS Lett. 587:873-879(2013).
CC -!- FUNCTION: Required for embryogenesis. May be involved with the
CC spliceosome. {ECO:0000269|PubMed:23454656}.
CC -!- SUBUNIT: Interacts with At5g63440. {ECO:0000269|PubMed:23454656}.
CC -!- INTERACTION:
CC F4I2J8; Q8GWQ6: At5g63440; NbExp=4; IntAct=EBI-8454629, EBI-8454619;
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:23454656}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4I2J8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4I2J8-2; Sequence=VSP_054997;
CC -!- DISRUPTION PHENOTYPE: Embryo lethality when homozygous.
CC {ECO:0000269|PubMed:23454656}.
CC -!- SIMILARITY: Belongs to the CACTIN family. {ECO:0000305}.
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DR EMBL; AC003027; AAD10685.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27632.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27633.1; -; Genomic_DNA.
DR PIR; H86169; H86169.
DR RefSeq; NP_001184904.1; NM_001197975.1. [F4I2J8-1]
DR RefSeq; NP_171887.1; NM_100272.4. [F4I2J8-2]
DR AlphaFoldDB; F4I2J8; -.
DR SMR; F4I2J8; -.
DR BioGRID; 24606; 2.
DR IntAct; F4I2J8; 4.
DR MINT; F4I2J8; -.
DR STRING; 3702.AT1G03910.2; -.
DR iPTMnet; F4I2J8; -.
DR PaxDb; F4I2J8; -.
DR PRIDE; F4I2J8; -.
DR ProteomicsDB; 239152; -. [F4I2J8-1]
DR EnsemblPlants; AT1G03910.1; AT1G03910.1; AT1G03910. [F4I2J8-2]
DR EnsemblPlants; AT1G03910.2; AT1G03910.2; AT1G03910. [F4I2J8-1]
DR GeneID; 839371; -.
DR Gramene; AT1G03910.1; AT1G03910.1; AT1G03910. [F4I2J8-2]
DR Gramene; AT1G03910.2; AT1G03910.2; AT1G03910. [F4I2J8-1]
DR KEGG; ath:AT1G03910; -.
DR Araport; AT1G03910; -.
DR TAIR; locus:2024182; AT1G03910.
DR eggNOG; KOG2370; Eukaryota.
DR HOGENOM; CLU_011759_1_0_1; -.
DR InParanoid; F4I2J8; -.
DR OMA; YFLEACH; -.
DR OrthoDB; 1252926at2759; -.
DR PRO; PR:F4I2J8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I2J8; baseline and differential.
DR Genevisible; F4I2J8; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IBA:GO_Central.
DR InterPro; IPR019134; Cactin_C.
DR InterPro; IPR018816; Cactin_central.
DR Pfam; PF10312; Cactin_mid; 2.
DR Pfam; PF09732; CactinC_cactus; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; mRNA processing; mRNA splicing; Nucleus;
KW Phosphoprotein; Reference proteome; Spliceosome.
FT CHAIN 1..716
FT /note="Cactin"
FT /id="PRO_0000429604"
FT REGION 1..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 153..201
FT /evidence="ECO:0000255"
FT COMPBIAS 1..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..99
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..500
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 510..525
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 450
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862,
FT ECO:0007744|PubMed:19376835"
FT VAR_SEQ 257..300
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054997"
SQ SEQUENCE 716 AA; 83434 MW; 22AB9CA80AB883A0 CRC64;
MGSHGKGKRD RSGRQKKRRD ESESGSESES YTSDSDGSDD LSPPRSSRRK KGSSSRRTRR
RSSSDDSSDS DGGRKSKKRS SSKDYSEEKV TEYMSKKAQK KALRAAKKLK TQSVSGYSND
SNPFGDSNLT ETFVWRKKIE KDVHRGVPLE EFSVKAEKRR HRERMTEVEK VKKRREERAV
EKARHEEEMA LLARERARAE FHDWEKKEEE FHFDQSKVRS EIRLREGRLK PIDVLCKHLD
GSDDLDIELS EPYMVFKKKK VRIGIWLNFQ LSITNVYVEA EYKNDSACLL LRSRVDILLN
KGLTVKDMEE LRDDIKMYLD LDRATPTRVQ YWEALIVVCD WELAEARKRD ALDRARVRGE
EPPAELLAQE RGLHAGVEAD VRKLLDGKTH AELVELQLDI ESQLRSGSAK VVEYWEAVLK
RLEIYKAKAC LKEIHAEMLR RHLHRLEQLS EGEDDVEVNP GLTRVVEENE EEINDTNLSD
AEEAFSPEPV AEEEEADEAA EAAGSFSPEL MHGDDREEAI DPEEDKKLLQ MKRMIVLEKQ
KKRLKEAMDS KPAPVEDNLE LKAMKAMGAM EEGDAIFGSN AEVNLDSEVY WWHDKYRPRK
PKYFNRVHTG YEWNKYNQTH YDHDNPPPKI VQGYKFNIFY PDLVDKIKAP IYTIEKDGTS
AETCMIRFHA GPPYEDIAFR IVNKEWEYSH KKGFKCTFER GILHLYFNFK RHRYRR
