YBCJ_ECOLI
ID YBCJ_ECOLI Reviewed; 70 AA.
AC P0AAS7; P45571; P77661; Q2MBQ1;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Uncharacterized protein YbcJ;
GN Name=ybcJ; OrderedLocusNames=b0528, JW5070;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Yonetani Y., Sanpei G., Mizobuchi K.;
RT "Cloning and nucleotide sequence analysis of a novel adenine-sensitive
RT mutation of Escherichia coli strain K-12 W3110.";
RL Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RX PubMed=1748668; DOI=10.1016/s0021-9258(18)54377-5;
RA D'Ari L., Rabinowitz J.C.;
RT "Purification, characterization, cloning, and amino acid sequence of the
RT bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-
RT methenyltetrahydrofolate cyclohydrolase from Escherichia coli.";
RL J. Biol. Chem. 266:23953-23958(1991).
RN [6]
RP IDENTIFICATION.
RX PubMed=7567469; DOI=10.1093/nar/23.17.3554;
RA Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.;
RT "Detection of new genes in a bacterial genome using Markov models for three
RT gene classes.";
RL Nucleic Acids Res. 23:3554-3562(1995).
RN [7]
RP STRUCTURE BY NMR, AND PROPOSED FUNCTION.
RC STRAIN=K12 / MC1061 / ATCC 53338 / DSM 7140;
RX PubMed=12837795; DOI=10.1128/jb.185.14.4204-4210.2003;
RA Volpon L., Lievre C., Osborne M.J., Gandhi S., Iannuzzi P., Larocque R.,
RA Cygler M., Gehring K., Ekiel I.;
RT "The solution structure of YbcJ from Escherichia coli reveals a recently
RT discovered alphaL motif involved in RNA binding.";
RL J. Bacteriol. 185:4204-4210(2003).
CC -!- FUNCTION: Its structure and the presence of conserved basic residues
CC indicates that it probably binds RNA.
CC -!- CAUTION: Although the solution structure of this protein has been
CC solved it has not been shown to be expressed in E.coli. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB40281.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=Ref.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D10588; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U82664; AAB40281.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73630.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76305.1; -; Genomic_DNA.
DR EMBL; M74789; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; G64784; G64784.
DR RefSeq; NP_415061.2; NC_000913.3.
DR RefSeq; WP_000190288.1; NZ_STEB01000007.1.
DR PDB; 1P9K; NMR; -; A=1-70.
DR PDBsum; 1P9K; -.
DR AlphaFoldDB; P0AAS7; -.
DR BMRB; P0AAS7; -.
DR SMR; P0AAS7; -.
DR BioGRID; 4262818; 30.
DR DIP; DIP-47878N; -.
DR IntAct; P0AAS7; 56.
DR STRING; 511145.b0528; -.
DR jPOST; P0AAS7; -.
DR PaxDb; P0AAS7; -.
DR PRIDE; P0AAS7; -.
DR EnsemblBacteria; AAC73630; AAC73630; b0528.
DR EnsemblBacteria; BAE76305; BAE76305; BAE76305.
DR GeneID; 66671177; -.
DR GeneID; 945158; -.
DR KEGG; ecj:JW5070; -.
DR KEGG; eco:b0528; -.
DR PATRIC; fig|1411691.4.peg.1750; -.
DR EchoBASE; EB2717; -.
DR eggNOG; COG2501; Bacteria.
DR HOGENOM; CLU_127162_1_2_6; -.
DR InParanoid; P0AAS7; -.
DR OMA; QGWSESG; -.
DR PhylomeDB; P0AAS7; -.
DR BioCyc; EcoCyc:EG12879-MON; -.
DR EvolutionaryTrace; P0AAS7; -.
DR PRO; PR:P0AAS7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0003723; F:RNA binding; ISS:EcoCyc.
DR GO; GO:0006412; P:translation; IMP:EcoCyc.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; RNA-binding.
FT CHAIN 1..70
FT /note="Uncharacterized protein YbcJ"
FT /id="PRO_0000168649"
FT DOMAIN 12..68
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:1P9K"
FT HELIX 14..21
FT /evidence="ECO:0007829|PDB:1P9K"
FT STRAND 25..28
FT /evidence="ECO:0007829|PDB:1P9K"
FT HELIX 32..39
FT /evidence="ECO:0007829|PDB:1P9K"
FT STRAND 55..61
FT /evidence="ECO:0007829|PDB:1P9K"
FT STRAND 64..69
FT /evidence="ECO:0007829|PDB:1P9K"
SQ SEQUENCE 70 AA; 7390 MW; 741FD495A73B08BA CRC64;
MATFSLGKHP HVELCDLLKL EGWSESGAQA KIAIAEGQVK VDGAVETRKR CKIVAGQTVS
FAGHSVQVVA
