CATIN_MOUSE
ID CATIN_MOUSE Reviewed; 772 AA.
AC Q9CS00;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Cactin;
GN Name=Cactin;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 362-772.
RC STRAIN=C57BL/6J; TISSUE=Liver;
RA Adachi J., Aizawa K., Akahira S., Akimura T., Arai A., Aono H., Arakawa T.,
RA Bono H., Carninci P., Fukuda S., Fukunishi Y., Furuno M., Hanagaki T.,
RA Hara A., Hayatsu N., Hiramoto K., Hiraoka T., Hori F., Imotani K.,
RA Ishii Y., Itoh M., Izawa M., Kasukawa T., Kato H., Kawai J., Kojima Y.,
RA Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T., Miyazaki A.,
RA Nishi K., Nomura K., Numazaki R., Ohno M., Okazaki Y., Okido T., Owa C.,
RA Saito H., Saito R., Sakai C., Sakai K., Sano H., Sasaki D., Shibata K.,
RA Shibata Y., Shinagawa A., Shiraki T., Sogabe Y., Suzuki H., Tagami M.,
RA Tagawa A., Takahashi F., Tanaka T., Tejima Y., Toya T., Yamamura T.,
RA Yasunishi A., Yoshida K., Yoshino M., Muramatsu M., Hayashizaki Y.;
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=20829348; DOI=10.1074/jbc.m110.139113;
RA Atzei P., Gargan S., Curran N., Moynagh P.N.;
RT "Cactin targets the MHC class III protein IkappaB-like (IkappaBL) and
RT inhibits NF-kappaB and interferon-regulatory factor signaling pathways.";
RL J. Biol. Chem. 285:36804-36817(2010).
CC -!- FUNCTION: Involved in the regulation of innate immune response (By
CC similarity). Acts as negative regulator of Toll-like receptor,
CC interferon-regulatory factor (IRF) and canonical NF-kappa-B signaling
CC pathways (By similarity). Contributes to the regulation of
CC transcriptional activation of NF-kappa-B target genes in response to
CC endogenous pro-inflammatory stimuli (By similarity).
CC {ECO:0000250|UniProtKB:Q8WUQ7}.
CC -!- SUBUNIT: Interacts (via N-terminal domain) with NFKBIL1; the
CC interaction occurs in a pro-inflammatory-independent manner (By
CC similarity). Does not interact with RELA NF-kappa-B subunit (By
CC similarity). Identified in the spliceosome C complex (By similarity).
CC {ECO:0000250|UniProtKB:Q8WUQ7}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8WUQ7}.
CC Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8WUQ7}. Note=Nuclear
CC localization with a speckled expression pattern in some cells.
CC Colocalizes with NFKBIL1 in the nucleus (By similarity).
CC {ECO:0000250|UniProtKB:Q8WUQ7}.
CC -!- TISSUE SPECIFICITY: Expressed in cortex, hippocampus, cerebellum,
CC heart, lung, kidney, liver, spleen and thymus.
CC {ECO:0000269|PubMed:20829348}.
CC -!- SIMILARITY: Belongs to the CACTIN family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC155937; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK010963; BAB27295.1; -; mRNA.
DR CCDS; CCDS35995.1; -.
DR RefSeq; NP_081657.1; NM_027381.2.
DR AlphaFoldDB; Q9CS00; -.
DR SMR; Q9CS00; -.
DR BioGRID; 213980; 28.
DR STRING; 10090.ENSMUSP00000059533; -.
DR iPTMnet; Q9CS00; -.
DR PhosphoSitePlus; Q9CS00; -.
DR EPD; Q9CS00; -.
DR jPOST; Q9CS00; -.
DR MaxQB; Q9CS00; -.
DR PaxDb; Q9CS00; -.
DR PRIDE; Q9CS00; -.
DR ProteomicsDB; 265542; -.
DR Antibodypedia; 52584; 89 antibodies from 19 providers.
DR Ensembl; ENSMUST00000050867; ENSMUSP00000059533; ENSMUSG00000034889.
DR GeneID; 70312; -.
DR KEGG; mmu:70312; -.
DR UCSC; uc007ghf.2; mouse.
DR CTD; 58509; -.
DR MGI; MGI:1917562; Cactin.
DR VEuPathDB; HostDB:ENSMUSG00000034889; -.
DR eggNOG; KOG2370; Eukaryota.
DR GeneTree; ENSGT00950000183102; -.
DR HOGENOM; CLU_011759_0_0_1; -.
DR InParanoid; Q9CS00; -.
DR OMA; HEPYMLL; -.
DR OrthoDB; 1252926at2759; -.
DR PhylomeDB; Q9CS00; -.
DR TreeFam; TF300906; -.
DR BioGRID-ORCS; 70312; 19 hits in 75 CRISPR screens.
DR ChiTaRS; Cactin; mouse.
DR PRO; PR:Q9CS00; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q9CS00; protein.
DR Bgee; ENSMUSG00000034889; Expressed in dorsal pancreas and 222 other tissues.
DR ExpressionAtlas; Q9CS00; baseline and differential.
DR Genevisible; Q9CS00; MM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; ISS:UniProtKB.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; ISS:UniProtKB.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB signaling; ISS:UniProtKB.
DR GO; GO:0045824; P:negative regulation of innate immune response; IBA:GO_Central.
DR GO; GO:0032688; P:negative regulation of interferon-beta production; ISS:UniProtKB.
DR GO; GO:0032717; P:negative regulation of interleukin-8 production; ISS:UniProtKB.
DR GO; GO:0031665; P:negative regulation of lipopolysaccharide-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
DR GO; GO:0001933; P:negative regulation of protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0034122; P:negative regulation of toll-like receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; ISS:UniProtKB.
DR GO; GO:0060339; P:negative regulation of type I interferon-mediated signaling pathway; ISS:UniProtKB.
DR InterPro; IPR019134; Cactin_C.
DR InterPro; IPR018816; Cactin_central.
DR Pfam; PF10312; Cactin_mid; 1.
DR Pfam; PF09732; CactinC_cactus; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Developmental protein; Immunity; Innate immunity;
KW Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Spliceosome; Ubl conjugation.
FT CHAIN 1..772
FT /note="Cactin"
FT /id="PRO_0000231618"
FT REGION 1..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 177..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 499..550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 157..207
FT /evidence="ECO:0000255"
FT COILED 247..299
FT /evidence="ECO:0000255"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..75
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..92
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 498
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WUQ7"
FT MOD_RES 573
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q8WUQ7"
FT CROSSLNK 491
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q8WUQ7"
FT CROSSLNK 506
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q8WUQ7"
SQ SEQUENCE 772 AA; 90661 MW; 4A4C6DF15D077D8B CRC64;
MRGAGRQMGR DSRSRSRSVG RRGRKQRSRS RGRSRSRSRS RSRSRSRSRS RSRSHGRSSR
RRREHERRRE RKRRSRGRRS DSEGEQRQKS RRRSQSLRPP RWHSQNQSSC SDSGEERAQG
SWARKGHRRS WSPGSSASSL DSPRRSRSPG TATLALSQQQ SLQERLRLRE ERKQQEELLK
AFETPEEKRA RRLAKKEAKE RKKREKMGWG EEYMGYTNTD NPFGDNNLLG TFIWNKALEK
KGISHLEEKE LKERNKRIQE DNRLELQKVK QLRLEREREK AMREQELELL QREKEAEHFK
TWEEQEDSFH LRQAKLRSKI RIRDGRAKPI DLLAKYISAE DDDLAVEMHE PYTFLNGLTV
ADMEDLLEDI QVYMELEQGK NVDFWRDMTT ITEDEIAKLR KLEASGKGPG ERREGVNASV
SSDVQSVFKG KTYNQLQVIF QGIEGKIRAG GPNLDMGYWE SLLQQLRAHM ARARLRERHQ
DVLRQKLFKL KQEQGVESEP LFPILKSEPS AAHSPEPEER PPSPGTSVDP VEPVEPEEAT
APGEAEGEAE GEAVLMEEDL IQQSLADYDA GRYSPRLLTA HELPLDAHVL EPHEDLQRLQ
LSRQQLQATG DASESAEDIF FRRAREGMGQ DEAQFSVEMP LGGRAYLWAD KYRPRKPRFF
NRVHTGFEWN KYNQTHYDFD NPPPKIVQGY KFNIFYPDLI RKRATPEYFL EACADNRDFA
ILRFHAGPPY EDIAFKIVSR EWEYSHRHGF RCQFANGIFQ LWFHFKRYRY RR
