ID   CATIN_TOXGV             Reviewed;         688 AA.
AC   B6KG46; V4Z8H8;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 2.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Cactin;
GN   Name=CACTIN; ORFNames=TGVEG_244380;
OS   Toxoplasma gondii (strain ATCC 50861 / VEG).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX   NCBI_TaxID=432359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 50861 / VEG;
RA   Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E.;
RT   "Annotation of Toxoplasma gondii VEG.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN CELL CYCLE REGULATION, SUBUNIT, AND MUTAGENESIS OF TYR-646.
RX   PubMed=22486860; DOI=10.1111/j.1365-2958.2012.08044.x;
RA   Szatanek T., Anderson-White B.R., Faugno-Fusci D.M., White M., Saeij J.P.,
RA   Gubbels M.J.;
RT   "Cactin is essential for G1 progression in Toxoplasma gondii.";
RL   Mol. Microbiol. 84:566-577(2012).
CC   -!- FUNCTION: Involved in the regulation of G1 progression and stage
CC       differentiation of tachyzoites. May play a role in gene expression.
CC       {ECO:0000269|PubMed:22486860}.
CC   -!- SUBUNIT: Homooligomer; the oligomerization requires the C-terminus.
CC       {ECO:0000269|PubMed:22486860}.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Note=Localizes in the nucleus throughout
CC       the entire tachyzoite cell cycle.
CC   -!- SIMILARITY: Belongs to the CACTIN family. {ECO:0000305}.
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DR   EMBL; AAYL02000165; ESS31603.1; -; Genomic_DNA.
DR   AlphaFoldDB; B6KG46; -.
DR   SMR; B6KG46; -.
DR   STRING; 5811.TGME49_044380; -.
DR   EnsemblProtists; ESS31603; ESS31603; TGVEG_244380.
DR   VEuPathDB; ToxoDB:TGVEG_244380; -.
DR   eggNOG; KOG2370; Eukaryota.
DR   HOGENOM; CLU_011759_0_0_1; -.
DR   OMA; HEPYMLL; -.
DR   Proteomes; UP000002226; Partially assembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0010971; P:positive regulation of G2/M transition of mitotic cell cycle; IMP:UniProtKB.
DR   GO; GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
DR   GO; GO:0045595; P:regulation of cell differentiation; IMP:UniProtKB.
DR   GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR   InterPro; IPR019134; Cactin_C.
DR   InterPro; IPR018816; Cactin_central.
DR   Pfam; PF10312; Cactin_mid; 1.
DR   Pfam; PF09732; CactinC_cactus; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Coiled coil; Differentiation; Nucleus; Reference proteome.
FT   CHAIN           1..688
FT                   /note="Cactin"
FT                   /id="PRO_0000419268"
FT   REGION          1..49
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          431..454
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          108..182
FT                   /evidence="ECO:0000255"
FT   COILED          248..275
FT                   /evidence="ECO:0000255"
FT   COILED          365..406
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        11..27
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         646
FT                   /note="Y->H: Does not affect nuclear localization. Reduces
FT                   self-oligomerization and inhibits G1 stage of the
FT                   tachyzoite cell cycle."
FT                   /evidence="ECO:0000269|PubMed:22486860"
SQ   SEQUENCE   688 AA;  79556 MW;  88049FE6E363BC56 CRC64;
     MGKKRRKHDG SGSSRSPSPD RIREARNPSQ AEVAGFRAPP SGKPAMTVKQ LREERQKLLH
     EHFGYSNTNN PFGDRLLAEP FVWKKKNQLL QAAGQKGKTT VAALIHSSKS KVQEIENVKR
     RREEREKEEE LMQQQREELQ REKEREHYEE YAQKEELFHR SNQLKKTEIR LEQNRAQLID
     LIVKGLRIIK GERFEKMDVL PGPPHEVLET YKNEPVTVAM LEDLIADVKL HIDVDTTVVD
     ADFQDFWKSL LVLAREALER AKKRKDVLDQ LARNSSPQMA AEAAAQLADT VCPQIRDADT
     GIVLSVAHEI EGLLGGKSAA ELDELEAQIR SKFEEEDIDV AYWEGILNKI PSFRARAVCL
     QTWHMMTQSA ADAEREMARE KALKEEEEAK AARAKGEELE GLLLEEADAR KLARAQQIKQ
     DAIKVARSVE KDVNPNADGS FSPELEPYDE GEVPPPVRGP YSPQLWPFDE FRHAEILAPD
     DDEDRRAILK KTLIQKERLK RAGLEEDPEP TPEAAENDAS LDVLDPAAKV TTYEEFVKKE
     KKNLEADEEV MASSSEHKLT MHYTWEEKYR PRKPRFFNRV KTGYSWNKYN TTHYDHDNPP
     PKVVQGYKFN IFYPDLIDKT KAPTWFLEPS DTPDTVIIRF HAGPPYEDIA FKILNQEWQL
     ERFRGFRNVF DRGIMQLYFS FKRYIYRR