YBDG_ECOLI
ID YBDG_ECOLI Reviewed; 415 AA.
AC P0AAT4; P39455; P77602;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Miniconductance mechanosensitive channel YbdG;
GN Name=ybdG; Synonyms=mscM; OrderedLocusNames=b0577, JW0566;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
RC STRAIN=B;
RX PubMed=7813889; DOI=10.1111/j.1574-6968.1994.tb07284.x;
RA Michael N.P., Brehm J.K., Anlezark G.M., Minton N.P.;
RT "Physical characterisation of the Escherichia coli B gene encoding
RT nitroreductase and its over-expression in Escherichia coli K12.";
RL FEMS Microbiol. Lett. 124:195-202(1994).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
RC STRAIN=K12 / C600 / CR34 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222;
RA Zenno S., Koike H., Tanokura M., Saigo K.;
RT "Gene cloning, purification, and characterization of NfsB, a minor oxygen-
RT insensitive nitroreductase from Escherichia coli, similar in biochemical
RT properties to FRaseI, the major flavin reductase in Vibrio fischeri.";
RL Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, INDUCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=K12;
RX PubMed=20616037; DOI=10.1073/pnas.1001405107;
RA Schumann U., Edwards M.D., Rasmussen T., Bartlett W., van West P.,
RA Booth I.R.;
RT "YbdG in Escherichia coli is a threshold-setting mechanosensitive channel
RT with MscM activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:12664-12669(2010).
RN [8]
RP SHOWS THAT THIS IS NOT MSCM.
RX PubMed=22874652; DOI=10.4161/chan.20998;
RA Edwards M.D., Black S., Rasmussen T., Rasmussen A., Stokes N.R.,
RA Stephen T.L., Miller S., Booth I.R.;
RT "Characterization of three novel mechanosensitive channel activities in
RT Escherichia coli.";
RL Channels 6:272-281(2012).
CC -!- FUNCTION: Mechanosensitive channel of miniconductance that confers
CC protection against mild hypoosmotic shock. Overexpression confers
CC protection against severe shocks. {ECO:0000269|PubMed:20616037}.
CC -!- SUBUNIT: Homoheptamer. {ECO:0000305|PubMed:20616037}.
CC -!- INTERACTION:
CC P0AAT4; P0AAT4: ybdG; NbExp=2; IntAct=EBI-1118850, EBI-1118850;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:20616037}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:20616037}.
CC -!- INDUCTION: Induced by growth at high osmolarity.
CC {ECO:0000269|PubMed:20616037}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be the miniconductance
CC mechanosensitive channel MscM. {ECO:0000305|PubMed:20616037}.
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DR EMBL; U82598; AAB40775.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73678.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35217.1; -; Genomic_DNA.
DR EMBL; U07860; AAC43265.1; -; Genomic_DNA.
DR EMBL; D25414; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; G64790; G64790.
DR RefSeq; NP_415109.1; NC_000913.3.
DR RefSeq; WP_001153148.1; NZ_SSZK01000024.1.
DR AlphaFoldDB; P0AAT4; -.
DR BioGRID; 4262912; 25.
DR DIP; DIP-51225N; -.
DR IntAct; P0AAT4; 4.
DR STRING; 511145.b0577; -.
DR TCDB; 1.A.23.4.5; the small conductance mechanosensitive ion channel (mscs) family.
DR jPOST; P0AAT4; -.
DR PaxDb; P0AAT4; -.
DR PRIDE; P0AAT4; -.
DR EnsemblBacteria; AAC73678; AAC73678; b0577.
DR EnsemblBacteria; BAA35217; BAA35217; BAA35217.
DR GeneID; 58463690; -.
DR GeneID; 946243; -.
DR KEGG; ecj:JW0566; -.
DR KEGG; eco:b0577; -.
DR PATRIC; fig|1411691.4.peg.1697; -.
DR EchoBASE; EB2504; -.
DR eggNOG; COG0668; Bacteria.
DR HOGENOM; CLU_045354_1_0_6; -.
DR InParanoid; P0AAT4; -.
DR OMA; PTWRLMS; -.
DR PhylomeDB; P0AAT4; -.
DR BioCyc; EcoCyc:G6323-MON; -.
DR PRO; PR:P0AAT4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IMP:EcoCyc.
DR GO; GO:0071470; P:cellular response to osmotic stress; IEP:EcoCyc.
DR GO; GO:0006970; P:response to osmotic stress; IGI:EcoliWiki.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR011066; MscS_channel_C.
DR InterPro; IPR023408; MscS_dom_sf.
DR InterPro; IPR030192; YbdG.
DR PANTHER; PTHR30414; PTHR30414; 1.
DR Pfam; PF00924; MS_channel; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
DR SUPFAM; SSF82689; SSF82689; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Ion channel; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..415
FT /note="Miniconductance mechanosensitive channel YbdG"
FT /id="PRO_0000168667"
FT TOPO_DOM 1..24
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..104
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..173
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..415
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 415 AA; 46603 MW; C9A8A8A41CDC8F28 CRC64;
MQDLISQVED LAGIEIDHTT SMVMIFGIIF LTAVVVHIIL HWVVLRTFEK RAIASSRLWL
QIITQNKLFH RLAFTLQGII VNIQAVFWLQ KGTEAADILT TCAQLWIMMY ALLSVFSLLD
VILNLAQKFP AASQLPLKGI FQGIKLIGAI LVGILMISLL IGQSPAILIS GLGAMAAVLM
LVFKDPILGL VAGIQLSAND MLKLGDWLEM PKYGADGAVI DIGLTTVKVR NWDNTITTIP
TWSLVSDSFK NWSGMSASGG RRIKRSISID VTSIRFLDED EMQRLNKAHL LKPYLTSRHQ
EINEWNRQQG STESVLNLRR MTNIGTFRAY LNEYLRNHPR IRKDMTLMVR QLAPGDNGLP
LEIYAFTNTV VWLEYESIQA DIFDHIFAIV EEFGLRLHQS PTGNDIRSLA GAFKQ
