ID   YBEM_ECOLI              Reviewed;         187 AA.
AC   P0DP64; P39874; P77104; P77192;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2017, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Putative protein YbeM;
GN   Name=ybeM; OrderedLocusNames=b4581, JW0621; ORFNames=b0626;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-4.
RC   STRAIN=K12 / EMG2;
RA   Robison K., O'Keeffe T., Church G.M.;
RL   Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Pseudogene resulting from a nucleotide deletion that
CC       introduces a premature stop codon at position 66. This is the C-
CC       terminal fragment. The intact protein (AC A0A140NCB4) hydrolyzes
CC       deaminated glutathione (dGSH, 2-oxoglutaramate) to alpha-ketoglutarate
CC       (alpha-KG) and cysteinylglycine, has less activity against alpha-
CC       ketoglutaramate (a-KGM) and no activity on glutathione or L-glutamine
CC       (By similarity). May function as a metabolite repair enzyme (By
CC       similarity). {ECO:0000250|UniProtKB:A0A140NCB4}.
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       NIT1/NIT2 family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
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DR   EMBL; U82598; AAB40826.1; -; Genomic_DNA.
DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAA35269.1; -; Genomic_DNA.
DR   EMBL; U38811; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; H64796; H64796.
DR   AlphaFoldDB; P0DP64; -.
DR   SMR; P0DP64; -.
DR   STRING; 316407.4062249; -.
DR   EnsemblBacteria; BAA35269; BAA35269; BAA35269.
DR   KEGG; ecj:JW0621; -.
DR   eggNOG; COG0388; Bacteria.
DR   HOGENOM; CLU_030130_1_2_6; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR001110; UPF0012_CS.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS01227; UPF0012; 1.
PE   5: Uncertain;
KW   Reference proteome.
FT   CHAIN           1..187
FT                   /note="Putative protein YbeM"
FT                   /id="PRO_0000213258"
FT   DOMAIN          1..163
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ   SEQUENCE   187 AA;  20482 MW;  18B5DD09806F9DC4 CRC64;
     MMTTILTIHV PSTPGRAWNM LVALQAGNIV ARYAKLHLYD AFAIQESRRV DAGNEIAPLL
     EVEGMKVGLM TCYDLRFPEL ALAQALQGAE ILVLPAAWVR GPLKEHHWST LLAARALDTT
     CYMVAAGECG NKNIGQSRII DPFGVTIAAA SEMPALIMAE VTPERVRQVR AQLPVLNNRR
     FAPPQLL