ID   YBEY_AQUAE              Reviewed;         150 AA.
AC   O67367;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Endoribonuclease YbeY {ECO:0000255|HAMAP-Rule:MF_00009};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00009};
GN   Name=ybeY {ECO:0000255|HAMAP-Rule:MF_00009}; OrderedLocusNames=aq_1354;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS).
RX   PubMed=12832766; DOI=10.1107/s0907444903011028;
RA   Oganesyan V., Busso D., Brandsen J., Chen S., Jancarik J., Kim R.,
RA   Kim S.-H.;
RT   "Structure of the hypothetical protein AQ_1354 from Aquifex aeolicus.";
RL   Acta Crystallogr. D 59:1219-1223(2003).
CC   -!- FUNCTION: Single strand-specific metallo-endoribonuclease involved in
CC       late-stage 70S ribosome quality control and in maturation of the 3'
CC       terminus of the 16S rRNA. {ECO:0000255|HAMAP-Rule:MF_00009}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00009};
CC       Note=Binds 1 zinc ion. {ECO:0000255|HAMAP-Rule:MF_00009};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00009}.
CC   -!- SIMILARITY: Belongs to the endoribonuclease YbeY family.
CC       {ECO:0000255|HAMAP-Rule:MF_00009}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000657; AAC07338.1; -; Genomic_DNA.
DR   PIR; D70417; D70417.
DR   RefSeq; NP_213931.1; NC_000918.1.
DR   RefSeq; WP_010880869.1; NC_000918.1.
DR   PDB; 1OZ9; X-ray; 1.89 A; A=1-150.
DR   PDBsum; 1OZ9; -.
DR   AlphaFoldDB; O67367; -.
DR   SMR; O67367; -.
DR   STRING; 224324.aq_1354; -.
DR   EnsemblBacteria; AAC07338; AAC07338; aq_1354.
DR   KEGG; aae:aq_1354; -.
DR   PATRIC; fig|224324.8.peg.1058; -.
DR   eggNOG; COG0319; Bacteria.
DR   HOGENOM; CLU_106710_3_3_0; -.
DR   InParanoid; O67367; -.
DR   OMA; RMRIHPL; -.
DR   OrthoDB; 1830156at2; -.
DR   EvolutionaryTrace; O67367; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.390.30; -; 1.
DR   HAMAP; MF_00009; Endoribonucl_YbeY; 1.
DR   InterPro; IPR023091; MetalPrtase_cat_dom_sf_prd.
DR   InterPro; IPR002036; YbeY.
DR   InterPro; IPR020549; YbeY_CS.
DR   PANTHER; PTHR46986; PTHR46986; 1.
DR   Pfam; PF02130; YbeY; 1.
DR   TIGRFAMs; TIGR00043; TIGR00043; 1.
DR   PROSITE; PS01306; UPF0054; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Endonuclease; Hydrolase; Metal-binding; Nuclease;
KW   Reference proteome; Ribosome biogenesis; rRNA processing; Zinc.
FT   CHAIN           1..150
FT                   /note="Endoribonuclease YbeY"
FT                   /id="PRO_0000102400"
FT   BINDING         115
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00009"
FT   BINDING         119
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00009"
FT   BINDING         125
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00009"
FT   STRAND          10..17
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   HELIX           22..35
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   STRAND          40..48
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   HELIX           50..61
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   STRAND          68..73
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   STRAND          81..90
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   HELIX           91..101
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   HELIX           105..120
FT                   /evidence="ECO:0007829|PDB:1OZ9"
FT   HELIX           129..147
FT                   /evidence="ECO:0007829|PDB:1OZ9"
SQ   SEQUENCE   150 AA;  17338 MW;  490C48DB6F952BCF CRC64;
     MSSTKRQKNR VLVKLKKRKV RKDKIEKWAE LALSALGLNN VELSVYITDD QEIRELNKTY
     RKKDKPTDVL SFPMGEEFGG YKILGDVVIS QDTAERQARE LGHSLEEEVK RLIVHGIVHL
     LGYDHEKGGE EEKKFRELEN YVLSKLSKAL