YBFK_BACSU
ID YBFK_BACSU Reviewed; 296 AA.
AC O31452; Q7DL48;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Carboxylesterase YbfK;
DE EC=3.1.1.1;
GN Name=ybfK; OrderedLocusNames=BSU02260;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Haga K., Liu H., Yasumoto K., Takahashi H., Yoshikawa H.;
RT "Sequence analysis of the 70kb region between 17 and 23 degree of the
RT Bacillus subtilis chromosome.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=11389736; DOI=10.1046/j.1432-1327.2001.02238.x;
RA Droege M.J., Bos R., Quax W.J.;
RT "Paralogous gene analysis reveals a highly enantioselective 1,2-O-
RT isopropylideneglycerol caprylate esterase of Bacillus subtilis.";
RL Eur. J. Biochem. 268:3332-3338(2001).
CC -!- FUNCTION: Shows carboxylesterase activity in vitro.
CC {ECO:0000269|PubMed:11389736}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC Evidence={ECO:0000269|PubMed:11389736};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR EMBL; AB006424; BAA33123.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12020.1; -; Genomic_DNA.
DR PIR; F69749; F69749.
DR RefSeq; NP_388108.1; NC_000964.3.
DR RefSeq; WP_003246261.1; NZ_JNCM01000030.1.
DR PDB; 4CCY; X-ray; 2.04 A; A/B=1-296.
DR PDBsum; 4CCY; -.
DR AlphaFoldDB; O31452; -.
DR SMR; O31452; -.
DR STRING; 224308.BSU02260; -.
DR ESTHER; bacsu-ybfk; 6_AlphaBeta_hydrolase.
DR PaxDb; O31452; -.
DR PRIDE; O31452; -.
DR EnsemblBacteria; CAB12020; CAB12020; BSU_02260.
DR GeneID; 938434; -.
DR KEGG; bsu:BSU02260; -.
DR PATRIC; fig|224308.179.peg.232; -.
DR eggNOG; COG0596; Bacteria.
DR InParanoid; O31452; -.
DR OMA; PTQCFAG; -.
DR PhylomeDB; O31452; -.
DR BioCyc; BSUB:BSU02260-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..296
FT /note="Carboxylesterase YbfK"
FT /id="PRO_0000360753"
FT ACT_SITE 129
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 244
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 273
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT HELIX 12..24
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 42..50
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 56..60
FT /evidence="ECO:0007829|PDB:4CCY"
FT TURN 63..65
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 67..70
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 71..80
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 81..86
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 104..118
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 130..141
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 146..153
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 164..170
FT /evidence="ECO:0007829|PDB:4CCY"
FT TURN 171..173
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 175..186
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 194..205
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 209..211
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 219..221
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 226..230
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 236..241
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 245..247
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 249..259
FT /evidence="ECO:0007829|PDB:4CCY"
FT STRAND 264..268
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 275..278
FT /evidence="ECO:0007829|PDB:4CCY"
FT HELIX 280..291
FT /evidence="ECO:0007829|PDB:4CCY"
SQ SEQUENCE 296 AA; 33071 MW; 7315DE166DDEF3BD CRC64;
MIQDSMQFAA VESGLRFYQA YDQSLSLWPI ESEAFYVSTR FGKTHIIASG PKDAPSLILL
HGGLFSSAMW YPNIAAWSSQ FRTYAVDIIG DKNKSIPSAA METRADFAEW MKDVFDSLGL
ETAHLAGLSL GGSHIVNFLL RAPERVERAV VISPAEAFIS FHPDVYKYAA ELTGARGAES
YIKWITGDSY DLHPLLQRQI VAGVEWQDEQ RSLKPTENGF PYVFTDQELK SIQVPVLLMF
GEHEAMYHQQ MAFERASVLV PGIQAEIVKN AGHLLSLEQP EYVNQRVLSF LCGGIK
