YBIO_ECOLI
ID YBIO_ECOLI Reviewed; 741 AA.
AC P75783; Q9R7S0; Q9R7S2; Q9ZBC5;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Moderate conductance mechanosensitive channel YbiO;
DE Flags: Precursor;
GN Name=ybiO; OrderedLocusNames=b0808, JW5108;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=22874652; DOI=10.4161/chan.20998;
RA Edwards M.D., Black S., Rasmussen T., Rasmussen A., Stokes N.R.,
RA Stephen T.L., Miller S., Booth I.R.;
RT "Characterization of three novel mechanosensitive channel activities in
RT Escherichia coli.";
RL Channels 6:272-281(2012).
CC -!- FUNCTION: Mechanosensitive channel that protects cells against
CC hypoosmotic stress when highly overexpressed.
CC {ECO:0000269|PubMed:22874652}.
CC -!- SUBUNIT: Homoheptamer. {ECO:0000269|PubMed:22874652}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:22874652}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:22874652}.
CC -!- INDUCTION: Induced by NaCl and RpoS. {ECO:0000269|PubMed:22874652}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR EMBL; U00096; AAC73895.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35474.2; -; Genomic_DNA.
DR PIR; H64817; H64817.
DR RefSeq; NP_415329.4; NC_000913.3.
DR RefSeq; WP_001267253.1; NZ_STEB01000019.1.
DR PDB; 7A46; EM; 3.00 A; A/B/C/D/E/F/G=1-741.
DR PDBsum; 7A46; -.
DR AlphaFoldDB; P75783; -.
DR SMR; P75783; -.
DR BioGRID; 4260824; 272.
DR DIP; DIP-11435N; -.
DR STRING; 511145.b0808; -.
DR TCDB; 1.A.23.3.2; the small conductance mechanosensitive ion channel (mscs) family.
DR jPOST; P75783; -.
DR PaxDb; P75783; -.
DR PRIDE; P75783; -.
DR EnsemblBacteria; AAC73895; AAC73895; b0808.
DR EnsemblBacteria; BAA35474; BAA35474; BAA35474.
DR GeneID; 945935; -.
DR KEGG; ecj:JW5108; -.
DR KEGG; eco:b0808; -.
DR PATRIC; fig|1411691.4.peg.1470; -.
DR EchoBASE; EB3104; -.
DR eggNOG; COG0668; Bacteria.
DR HOGENOM; CLU_013626_2_0_6; -.
DR InParanoid; P75783; -.
DR OMA; WGVGRAY; -.
DR PhylomeDB; P75783; -.
DR BioCyc; EcoCyc:G6417-MON; -.
DR PRO; PR:P75783; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR GO; GO:0071470; P:cellular response to osmotic stress; IBA:GO_Central.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR011066; MscS_channel_C.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR InterPro; IPR023408; MscS_dom_sf.
DR InterPro; IPR045276; YbiO_bact.
DR PANTHER; PTHR30460; PTHR30460; 1.
DR Pfam; PF00924; MS_channel; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
DR SUPFAM; SSF82689; SSF82689; 1.
DR SUPFAM; SSF82861; SSF82861; 1.
DR PROSITE; PS01246; UPF0003; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Ion channel;
KW Ion transport; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..741
FT /note="Moderate conductance mechanosensitive channel YbiO"
FT /id="PRO_0000110241"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 509..529
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 22..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 518..532
FT /evidence="ECO:0007829|PDB:7A46"
FT HELIX 539..570
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 579..582
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 585..592
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 594..601
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 606..610
FT /evidence="ECO:0007829|PDB:7A46"
FT HELIX 611..613
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 615..620
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 625..634
FT /evidence="ECO:0007829|PDB:7A46"
FT HELIX 639..655
FT /evidence="ECO:0007829|PDB:7A46"
FT HELIX 657..660
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 668..675
FT /evidence="ECO:0007829|PDB:7A46"
FT STRAND 677..689
FT /evidence="ECO:0007829|PDB:7A46"
FT HELIX 693..710
FT /evidence="ECO:0007829|PDB:7A46"
SQ SEQUENCE 741 AA; 81904 MW; 69B0A27678976DB5 CRC64;
MRWILFILFC LLGAPAHAVS IPGVTTTTTT DSTTEPAPEP DIEQKKAAYG ALADVLDNDT
SRKELIDQLR TVAATPPAEP VPKIVPPTLV EEQTVLQKVT EVSRHYGEAL SARFGQLYRN
ITGSPHKPFN PQTFSNALTH FSMLAVLVFG FYWLIRLCAL PLYRKMGQWA RQKNRERSNW
LQLPAMIIGA FIIDLLLLAL TLFVGQVLSD NLNAGSRTIA FQQSLFLNAF ALIEFFKAVL
RLIFCPNVAE LRPFTIQDES ARYWSRRLSW LSSLIGYGLI VAVPIISNQV NVQIGALANV
IIMLCMTVWA LYLIFRNKKE ITQHLLNFAE HSLAFFSLFI RAFALVWHWL ASAYFIVLFF
FSLFDPGNSL KFMMGATVRS LAIIGIAAFV SGMFSRWLAK TITLSPHTQR NYPELQKRLN
GWLSAALKTA RILTVCVAVM LLLSAWGLFD FWNWLQNGAG QKTVDILIRI ALILFFSAVG
WTVLASLIEN RLASDIHGRP LPSARTRTLL TLFRNALAVI ISTITIMIVL SEIGVNIAPL
LAGAGALGLA ISFGSQTLVK DIITGVFIQF ENGMNTGDLV TIGPLTGTVE RMSIRSVGVR
QDTGAYHIIP WSSITTFANF VRGIGSVVAN YDVDRHEDAD KANQALKDAV AELMENEEIR
GLIIGEPNFA GIVGLSNTAF TLRVSFTTLP LKQWTVRFAL DSQVKKHFDL AGVRAPVQTY
QVLPAPGATP AEPLPPGEPT L
